2024/06/28 更新

サトウ ユウスケ
佐藤 佑介
SATO Yusuke
Scopus 論文情報  
総論文数: 0  総Citation: 0  h-index: 10

Citation Countは当該年に発表した論文の被引用数

外部リンク

研究キーワード

  • 分子ロボティクス,DNAナノテクノロジー ,生物物理,人工細胞

研究分野

  • ナノテク・材料 / ナノマイクロシステム

  • 情報通信 / 生命、健康、医療情報学

出身学校

  • 2013年03月   東北大学   工学部   機械知能航空工学科   卒業   日本国

出身大学院

  • 2018年03月   東北大学   工学研究科   バイオロボティクス専攻   博士課程・博士後期課程   修了   日本国

取得学位

  • 東北大学  -  博士(工学)   2018年03月

  • 東北大学  -  修士(工学)   2015年03月

学外略歴

  • 2021年07月 - 2022年03月   東北大学   プロミネントリサーチフェロー

  • 2020年03月 - 2022年03月   東北大学   学際科学フロンティア研究所新領域創成研究部   助教

  • 2019年08月 - 2019年12月   Kent State University   Department of Physics   研究員   アメリカ合衆国

  • 2018年04月 - 2020年02月   東京工業大学   情報理工学院   日本学術振興会特別研究員

所属学会・委員会

  • 2020年10月 - 現在   情報計算化学生物学会   日本国

  • 2020年03月 - 現在   計測自動制御学会   日本国

  • 2018年09月 - 現在   化学とマイクロ・ナノシステム学会   日本国

  • 2015年09月 - 現在   細胞を創る研究会   日本国

  • 2012年05月 - 現在   生物物理学会   日本国

論文

  • Autonomous assembly and disassembly of gliding molecular robots regulated by a DNA-based molecular controller 査読有り

    Ibuki Kawamata, Kohei Nishiyama, Daiki Matsumoto, Shosei Ichiseki, Jakia J. Keya Kohei Okuyama, Masatoshi Ichikawa, Arif Md. Rashedul Kabir, Yusuke Sato, Daisuke Inoue, Satoshi Murata, Kazuki Sada, Akira Kakugo, Shin-ichiro M. Nomura

    Science Advances   2024年05月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1126/sciadv.adn4490

    Kyutacar

  • Artificial Molecular Systems for Complex Functions Based on DNA Nanotechnology and Cell-Sized Lipid Vesicles 招待有り 査読有り 国際誌

    Yusuke Sato

    ChemSystemsChem   2024年05月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    DOI: 10.1002/syst.202400021

    Kyutacar

  • Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation 招待有り 査読有り

    Yusuke Sato, Masahiro Takinoue

    Biophysics and Physicobiology   2024年02月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    DOI: https://doi.org/10.2142/biophysico.bppb-v21.0010

  • Design of artificial molecular motor inheriting directionality and scalability 査読有り

    Ito K.I., Sato Y., Toyabe S.

    Biophysical Journal   2024年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    Realizing artificial molecular motors with autonomous functionality and high performance is a major challenge in biophysics. Such motors not only provide new perspectives in biotechnology but also offer a novel approach for the bottom-up elucidation of biological molecular motors. Directionality and scalability are critical factors for practical applications. However, the simultaneous realization of both remains challenging. In this study, we propose a novel design for a rotary motor that can be fabricated using a currently available technology, DNA origami, and validate its functionality through simulations with practical parameters. We demonstrate that the motor rotates unidirectionally and processively in the direction defined by structural asymmetry, which induces kinetic asymmetry in motor motion. The motor also exhibits scalability, such that increasing the number of connections between the motor and stator allows for a larger speed, run length, and stall force.

    DOI: 10.1016/j.bpj.2024.02.026

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85187566361&origin=inward

  • Pioneering artificial cell-like structures with DNA nanotechnology-based liquid-liquid phase separation 査読有り

    Sato Y., Takinoue M.

    Biophysics and physicobiology   21 ( 1 )   2024年01月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    Recent studies have revealed that liquid-liquid phase separation (LLPS) plays crucial roles in various cellular functions. Droplets formed via LLPS within cells, often referred to as membraneless organelles, serve to concentrate specific molecules, thus enhancing biochemical reactions. Artificial LLPS systems have been utilized to construct synthetic cell models, employing a range of synthetic molecules. LLPS systems based on DNA nanotechnology are particularly notable for their designable characteristics in droplet formation, dynamics, properties, and functionalities. This review surveys recent advancements in DNA-based LLPS systems, underscoring the programmability afforded by DNA’s base-pair specific interactions. We discuss the fundamentals of DNA droplet formation, including temperature-dependence and physical properties, along with the precise control achievable through sequence design. Attention is given to the phase separation of DNA nanostructures on two-dimensional closed interfaces, which results in spatial pattern formation at the interface. Furthermore, we spotlight the potential of DNA droplet computing for cancer diagnostics through specific microRNA pattern recognition. We envision that DNA-based LLPS presents a versatile platform for the exploration of cellular mimicry and opens innovative ways for the development of functional synthetic cells.

    DOI: 10.2142/biophysico.bppb-v21.0010

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85185697860&origin=inward

  • Sequence-dependent fusion dynamics and physical properties of DNA droplets 査読有り 国際誌

    Yusuke Sato, Masahiro Takinoue

    Nanoscale Advances   5   1919 - 1925   2023年02月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1039/D3NA00073G

  • Surfactant-Assisted Purification of Hydrophobic DNA Nanostructures 査読有り

    Shoji Iwabuchi, Shin-ichiro M Nomura, Yusuke Sato

    ChemBioChem   2022年12月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

  • DNA Droplets: Intelligent, Dynamic Fluid 招待有り 査読有り

    Hirotake Udono, Jing Gong, Yusuke Sato, Masahiro Takinoue

    Advanced Biology   2022年12月

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    記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • 相分離 DNA 液滴によるパターン形成と情報 処理 招待有り

    佐藤 佑介,瀧ノ上正浩

    生物物理   62 ( 6 )   345 - 347   2022年11月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    DOI: 10.2142/biophys.62.345

  • 自律型分子コンピューテ ィングの現状と未来 招待有り

    佐藤佑介, 公婧, 瀧ノ上正浩

    高分子   71   522 - 524   2022年10月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Water-in-water droplets selectively uptake self-assembled DNA nano/microstructures: a versatile method for purification in DNA nanotechnology 査読有り

    Marcos Masukawa,Yusuke Sato,Fujio Yu,Kanta Tsumoto,Kenichi Yoshikawa,Masahiro Takinoue

    ChemBioChem   23 ( 17 )   2022年06月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DNA is an excellent material for constructing self-assembled nano/microstructures. Owing to the widespread use of DNA as a building block in laboratories and industry, it is desirable to increase the efficiency of all steps involved in producing self-assembled DNA structures. One of the bottlenecks is the purification required to separate the excess components from the target structures. This paper describes a purification method based on the fractionation by water-in-water (W/W) droplets composed of phase-separated dextran-rich droplets in a polyethylene glycol (PEG)-rich continuous phase. The dextran-rich droplets facilitate the selective uptake of self-assembled DNA nano/microstructures and allow the separation of the target structure. This study investigates the ability to purify DNA origami, DNA hydrogels, and DNA microtubes. The W/W-droplet fractionation allows the purification of structures of a broad size spectrum without changes to the protocol. By quantifying the activity of deoxyribozyme-modified DNA origami after W/W-droplet purification, this study demonstrates that this method sufficiently preserves the accessibility to the surface of a functional DNA nanostructure. It is considered that the W/W-droplet fractionation could become one of the standard methods for the purification of self-assembled DNA nano/microstructures for biomedical and nanotechnology applications owing to its low cost and simplicity.

    DOI: 10.1002/cbic.202200240

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85133208810&origin=inward

  • 人工細胞型分子ロボットへの道

    岩渕祥璽, 深見実希, 佐藤佑介, 野村 M, 慎一郎

    生物物理   2022年06月

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    記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Computational DNA Droplets Recognizing miRNA Sequence Inputs Based on Liquid–Liquid Phase Separation 査読有り 国際誌

    Gong J., Tsumura N., Sato Y., Takinoue M.

    Advanced Functional Materials   32 ( 37 )   2022年05月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    Phase-separated biomolecular droplets are formed in cells to regulate various biological processes. This phenomenon can be applied to constructing self-assembled dynamic molecular systems such as artificial cells and molecular robots. Recently, programmable phase-separated droplets called DNA droplets have been reported as a possible method to construct such dynamic molecular systems. This study reports a computational DNA droplet that can recognize a specific combination of tumor biomarker microRNAs (miRNAs) as molecular inputs and output a DNA logic computing result by physical DNA droplet phase separation. A mixed DNA droplet consisting of three DNA nanostructures with orthogonal sticky-end sequences and two linker DNAs to cross-bridge the orthogonal DNA nanostructures is proposed. By the hybridization of miRNAs with the linkers, the cross-bridging ability is lost, causing the phase-separation of the mixed DNA droplet into three DNA droplets, resulting in executing a miRNA pattern recognition described by a logical expression ((miRNA-1 ∧ miRNA-2) ∧ (miRNA-3 ∧ ¬miRNA-4)). This experimentally demonstrates that the computational DNA droplets recognize the above specific pattern of chemically synthesized miRNA sequences as a model experiment. In the future, this method will provide potential applications such as diagnosis and therapy with integration to biomolecular robots and artificial cells.

    DOI: 10.1002/adfm.202202322

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85129844345&origin=inward

  • Therapeutic Applications of Programmable DNA Nanostructures 招待有り 査読有り

    Aye S.L., Sato Y.

    Micromachines   13 ( 2 )   2022年02月

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    担当区分:最終著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    Deoxyribonucleic acid (DNA) nanotechnology, a frontier in biomedical engineering, is an emerging field that has enabled the engineering of molecular-scale DNA materials with applications in biomedicine such as bioimaging, biodetection, and drug delivery over the past decades. The programmability of DNA nanostructures allows the precise engineering of DNA nanocarriers with controllable shapes, sizes, surface chemistries, and functions to deliver therapeutic and functional payloads to target cells with higher efficiency and enhanced specificity. Programmability and control over design also allow the creation of dynamic devices, such as DNA nanorobots, that can react to external stimuli and execute programmed tasks. This review focuses on the current findings and progress in the field, mainly on the employment of DNA nanostructures such as DNA origami nanorobots, DNA nanotubes, DNA tetrahedra, DNA boxes, and DNA nanoflowers in the biomedical field for therapeutic purposes. We will also discuss the fate of DNA nanostructures in living cells, the major obstacles to overcome, that is, the stability of DNA nanostructures in biomedical applications, and the opportunities for DNA nanostructure-based drug delivery in the future.

    DOI: 10.3390/mi13020315

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85125016181&origin=inward

  • Capsule-like DNA Hydrogels with Patterns Formed by Lateral Phase Separation of DNA Nanostructures 査読有り 国際誌

    Yusuke Sato, Masahiro Takinoue

    JACS Au ( American Chemical Society (ACS) )   2 ( 1 )   159 - 168   2021年11月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    Phase separation is a key phenomenon in artificial cell construction. Recent studies have shown that the liquid-liquid phase separation of designed-DNA nanostructures induces the formation of liquid-like condensates that eventually become hydrogels by lowering the solution temperature. As a compartmental capsule is an essential artificial cell structure, many studies have focused on the lateral phase separation of artificial lipid vesicles. However, controlling phase separation using a molecular design approach remains challenging. Here, we present the lateral liquid-liquid phase separation of DNA nanostructures that leads to the formation of phase-separated capsule-like hydrogels. We designed three types of DNA nanostructures (two orthogonal and a linker nanostructure) that were adsorbed onto an interface of water-in-oil (W/O) droplets via electrostatic interactions. The phase separation of DNA nanostructures led to the formation of hydrogels with bicontinuous, patch, and mix patterns, due to the immiscibility of liquid-like DNA during the self-assembly process. The frequency of appearance of these patterns was altered by designing DNA sequences and altering the mixing ratio of the nanostructures. We constructed a phase diagram for the capsule-like DNA hydrogels by investigating pattern formation under various conditions. The phase-separated DNA hydrogels did not only form on the W/O droplet interface but also on the inner leaflet of lipid vesicles. Notably, the capsule-like hydrogels were extracted into an aqueous solution, maintaining the patterns formed by the lateral phase separation. In addition, the extracted hydrogels were successfully combined with enzymatic reactions, which induced their degradation. Our results provide a method for the design and control of phase-separated hydrogel capsules using sequence-designed DNAs. We envision that by incorporating various DNA nanodevices into DNA hydrogel capsules, the capsules will gain molecular sensing, chemical-information processing, and mechanochemical actuating functions, allowing the construction of functional molecular systems.

    DOI: 10.1021/jacsau.1c00450

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85128299469&origin=inward

  • DNA computing droplet to detect miRNAs for cancer diagnosis 査読有り

    Jing Gong, Nozomi Tsumura, Yusuke Sato, Masahiro Takinoue

    MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences   735 - 736   2021年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

  • Assembly and purification of DNA structures in aqueous-aqueous two-phase emulsion 査読有り

    Marcos Masukawa, Yukiko Okuda, Fujio Yu, Yusuke Sato, Kanta Tsumoto, Kenichi Yoshikawa, Masahiro Takinoue

    MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences   995 - 996   2021年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

  • 大学の枠を越えたオンライン生体分子デザインコンペティションの取り組み 査読有り

    村田 智, 葛谷 明紀, 藤原 慶, 平 順一, 川又 生吹, 佐藤 佑介, 瀧ノ上 正浩, 野村M. 慎一郎, 角五 彰, 堀 豊, 安部 桂太

    工学教育 ( 日本工業教育協会 )   69 ( 4 )   31 - 39   2021年07月

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    記述言語:日本語   掲載種別:研究論文(学術雑誌)

    DOI: https://doi.org/10.4307/jsee.69.4_31

  • DNA nanotechnology provides an avenue for the construction of programmable dynamic molecular systems 招待有り 査読有り

    Yusuke Sato, Yuki Suzuki

    Biophysics and Physicobiology ( 一般社団法人 日本生物物理学会 )   18 ( 0 )   116 - 126   2021年05月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    <p>Self-assembled supramolecular structures in living cells and their dynamics underlie various cellular events, such as endocytosis, cell migration, intracellular transport, cell metabolism, and gene expression. Spatiotemporally regulated association/dissociation and generation/degradation of assembly components is one of the remarkable features of biological systems. The significant advancement in DNA nanotechnology over the last few decades has enabled the construction of various-shaped nanostructures via programmed self-assembly of sequence-designed oligonucleotides. These nanostructures can further be assembled into micrometer-sized structures, including ordered lattices, tubular structures, macromolecular droplets, and hydrogels. In addition to being a structural material, DNA is adopted to construct artificial molecular circuits capable of activating/inactivating or producing/decomposing target DNA molecules based on strand displacement or enzymatic reactions. In this review, we provide an overview of recent studies on artificially designed DNA-based self-assembled systems that exhibit dynamic features, such as association/dis­sociation of components, phase separation, stimulus responsivity, and DNA circuit-regulated structural formation. These biomacromolecule-based, bottom-up approaches for the construction of artificial molecular systems will not only throw light on bio-inspired nano/micro engineering, but also enable us to gain insights into how autonomy and adaptability of living systems can be realized.</p>

    DOI: 10.2142/biophysico.bppb-v18.013

    Scopus

    CiNii Article

    CiNii Research

    その他リンク: https://www.jstage.jst.go.jp/article/biophysico/18/0/18_bppb-v18.013/_pdf

  • DNAナノテクノロジーによる細胞型分子ロボットの構築 招待有り

    佐藤 佑介, 瀧ノ上 正浩

    電気学会誌 ( 一般社団法人 電気学会 )   140 ( 9 )   582 - 584   2020年09月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    <p>1.はじめに</p><p>細胞は,機能を持った分子や超分子で構成された天然の分子システムである。そのようなシステムを人工的に作るアプローチの一つとして,細胞型分子ロボット<sup>(1)</sup>の構築に注目が集まっている。細胞型分子ロボットは,機能性分子デバイスをシステムとして組み上げたものである。分子レ</p>

    DOI: 10.1541/ieejjournal.140.582

    CiNii Article

    CiNii Research

    その他リンク: https://www.jstage.jst.go.jp/article/ieejjournal/140/9/140_582/_pdf

  • Sequence-based engineering of dynamic functions of micrometer-sized DNA droplets 査読有り

    Sato Y., Sakamoto T., Takinoue M.

    Science Advances   6 ( 23 )   2020年06月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    DNA has the potential to achieve a controllable macromolecular structure, such as hydrogels or droplets formed through liquid-liquid phase separation (LLPS), as the design of its base sequence can result in programmable interactions. Here, we constructed "DNA droplets"via LLPS of sequence-designed DNA nanostructures and controlled their dynamic functions by designing their sequences. Specifically, we were able to adjust the temperature required for the formation of DNA droplets by designing the sequences. In addition, the fusion, fission, and formation of Janus-shaped droplets were controlled by sequence design and enzymatic reactions. Furthermore, modifications of proteins with sequence-designed DNAs allowed for their capture into specific droplets. Overall, our results provide a platform for designing and controlling macromolecular droplets via the information encoded in component molecules and pave the way for various applications of sequence-designed DNA such as cell mimics, synthetic membraneless organelles, and artificial molecular systems.

    DOI: 10.1126/sciadv.aba3471

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086625101&origin=inward

  • Session 1SCA—Utilizing soft compartments/interfaces for the creation of artificial biosystems 査読有り

    Sato Y., Morita M., Suzuki Y.

    Biophysical Reviews   12 ( 2 )   257 - 259   2020年04月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(その他)

    DOI: 10.1007/s12551-020-00647-y

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85079722188&origin=inward

  • Photolithographic shape control of DNA hydrogels by photo-activated self-assembly of DNA nanostructures 査読有り

    Kasahara Y., Sato Y., Masukawa M.K., Okuda Y., Takinoue M.

    APL Bioengineering   4 ( 1 )   2020年03月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    We report a photolithographic method for the shape control of DNA hydrogels based on photo-activated self-assembly of Y-shaped DNA nanostructures (Y-motifs). To date, various methods to control the shape of DNA hydrogels have been developed to enhance the functions of the DNA hydrogel system. However, photolithographic production of shape-controlled DNA hydrogels formed through the self-assembly of DNA nanostructures without the use of radical polymerizations has never been demonstrated, although such a method is expected to be applied for the shape-control of DNA hydrogels encapsulating sensitive biomolecules, such as proteins. In this study, we used a photo-activated linker to initiate the self-assembly of Y-motifs, where the cross-linker DNA was at first inactive but was activated after UV light irradiation, resulting in the formation of shape-controlled DNA hydrogels only at the UV-exposed area produced by photomasks. We believe that this method will be applied for the construction of biohybrid machines, such as molecular robots and artificial cells that contain intelligent biomolecular devices, such as molecular sensors and computers.

    DOI: 10.1063/1.5132929

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85089534591&origin=inward

  • DNA hydrogels as a scaffold for organs-on-chips: Study of buoyancy and sedimentation of microbeads in DNA gels 査読有り

    Belot E., Tauran Y., Sato Y., Takinoue M., Brioude A., Fujii T., Genot A.J.

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1057 - 1058   2020年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Hydrogels lie at the heart of the organ-on-chip approach. Their physicochemistry and rheology largely determine the outcome of tissue engineering. DNA hydrogels are emerging as a promising medium due to their biocompatibility and physicochemical programmability. However their use as a scaffold for tissue engineering has been little studied. Here we study the buoyancy and sedimentation of microbeads suspended in DNA gels and culture medium. We identify the parameters (binding strengths and concentration of DNA strands) that should enable stable suspension and culture of cells.

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85098239881&origin=inward

  • Mapping the phase diagram of DNA hydrogels with droplet microfluidics 査読有り

    Mariette G., Lobato-Dauzier N., Deteix R., Zhang M., Okumura S., Sato Y., Takinoue M., Fujii T., Genot A.J.

    MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences   266 - 267   2020年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    DNA hydrogels have emerged in the recent decade as a powerful substrate to program soft matter at the nanoscale. In particular, DNA hydrogels exhibit liquid/liquid phase separation in vitro, a phenomenon that is appearing to be ubiquitous in cells. Yet, a quantitative understanding of their physics is missing. Here we use a droplet microfluidics platform to prepare tens of thousands of droplets with DNA motifs inside.

    Scopus

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  • DNA origami "quick" refolding inside of a micron-sized compartment 査読有り

    Watanabe T., Sato Y., Otaka H., Kawamata I., Murata S., Nomura S.I.M.

    Molecules   25 ( 1 )   2020年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    Investigations into the refolding of DNA origami leads to the creation of reconstructable nanostructures and deepens our understanding of the sustainability of life. Here, we report the refolding of the DNA origami structure inside a micron-sized compartment. In our experiments, conventional DNA origami and truss-type DNA origami were annealed and purified to remove the excess staples in a test tube. The DNA origami was then encapsulated inside of a micron-sized compartment of water-in-oil droplets, composed of neutral surfactants. The re-annealing process was then performed to initiate refolding in the compartment. The resulting 100-nm-sized DNA nanostructures were observed using atomic force microscopy (AFM), and the qualities of their structures were evaluated based on their shape. We found that the refolding of the DNA origami structure was favored inside the droplets compared with refolding in bulk solution. The refolded structures were able to fold even under "quick" one-minute annealing conditions. In addition, the smaller droplets (average diameter: 1.2 μm) appeared to be more advantageous for the refolding of the origamis than larger droplets. These results are expected to contribute to understanding the principles of life phenomena based on multimolecular polymer self-assembly in a micron-sized compartment, and for the production and maintenance of artificially designed molecules.

    DOI: 10.3390/molecules25010008

    Scopus

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  • 配列設計と相転移に基づいたDNAマイクロ構造の構築と制御 招待有り

    佐藤佑介, 瀧ノ上正浩

    化学とマイクロ・ナノシステム   18 ( 1 )   24 - 27   2019年03月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • 相分離脂質膜上におけるDNAナノ構造の環境依存的自己集合 招待有り

    佐藤 佑介

    生物物理   59 ( 4 )   212 - 213   2019年02月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Creation of artificial cell-like structures promoted by microfluidics technologies 招待有り 査読有り

    Sato Y., Takinoue M.

    Micromachines   10 ( 4 )   2019年01月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    The creation of artificial cells is an immensely challenging task in science. Artificial cells contribute to revealing the mechanisms of biological systems and deepening our understanding of them. The progress of versatile biological research fields has clarified many biological phenomena, and various artificial cell models have been proposed in these fields. Microfluidics provides useful technologies for the study of artificial cells because it allows the fabrication of cell-like compartments, including water-in-oil emulsions and giant unilamellar vesicles. Furthermore, microfluidics also allows the mimicry of cellular functions with chip devices based on sophisticated chamber design. In this review, we describe contributions of microfluidics to the study of artificial cells. Although typical microfluidic methods are useful for the creation of artificial-cell compartments, recent methods provide further benefits, including low-cost fabrication and a reduction of the sample volume. Microfluidics also allows us to create multi-compartments, compartments with artificial organelles, and on-chip artificial cells. We discuss these topics and the future perspective of microfluidics for the study of artificial cells and molecular robotics.

    DOI: 10.3390/MI10040216

    Scopus

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  • Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles 査読有り

    Sato Y., Komiya K., Kawamata I., Murata S., Nomura S.I.M.

    Chemical Communications   55 ( 62 )   9084 - 9087   2019年01月

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    担当区分:筆頭著者, 責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

    DOI: 10.1039/c9cc03277k

    Scopus

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  • Creation of DNA microdroplets based on phase transition and sequence design 査読有り

    Sato Y., Sakamoto T., Takinoue M.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   16 - 17   2019年01月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    DNA is a promising materials to design, construct, and control micrometer-sized structures. A few studies have focused on the phase transition of DNA microstructures. However, there is less knowledge of the effects of the base sequence of DNA on the phase behavior. Here, we reports DNA microdroplets that is a novel and unique DNA microstructure. We designed sequences of DNAs and successfully visuzalized the formation of DNA microdroplets via phase transition. We believe that our results provide new aspects of DNA as a soft/fluid material, which would contribute to various fields such as bioengineering, microfluidics, and artificial cell engineering.

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094941645&origin=inward

  • 分子ロボティクス:アメーバ型分子ロボットの開発 招待有り

    佐藤 佑介,野村 M. 慎一郎

    生化学   90 ( 5 )   728 - 733   2018年10月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Environment-Dependent Self-Assembly of DNA Origami Lattices on Phase-Separated Lipid Membranes 査読有り

    Sato Y., Endo M., Morita M., Takinoue M., Sugiyama H., Murata S., Nomura S., Suzuki Y.

    Advanced Materials Interfaces   5 ( 14 )   2018年07月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    Self-assembly of designed nanostructures on a lipid membrane offers a powerful approach for enhancing the functionality of its surface. Here, the environment-dependent assembly of blunt-ended DNA origami structures on a phase-separated lipid-bilayer membrane consisting of liquid-disordered (Ld) and solid-ordered (So) phases is reported. Fluorescence microscopy and atomic force microscopy (AFM) imaging reveal that DNA origami structures preferentially bind to the So phase. At higher concentrations, blunt-ended origamis form 2D lattices on the Ld phase through surface-mediated self-assembly. In contrast, those adsorbed on the So phase are less mobile and pack into aggregates. However, this situation is changed by adding NaCl. High-speed AFM imaging reveals the mechanism underlying the NaCl-induced changes, wherein the lattices on the Ld phase desorb from the surface while the packed aggregates on the So phase reorganize into the lattices. These results indicate that the formation of 2D lattices depends on the phase of the lipids; however, it can be tuned by ionic conditions, enabling us to select the domain on which the lattice is formed. The present results may guide the creation of membrane-supported DNA nanostructures that self-assemble into functional states in a lipid phase or ion-responsive manner.

    DOI: 10.1002/admi.201800437

    Scopus

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  • 動け、アメーバ型分子ロボット 招待有り

    佐藤 佑介,野村 M. 慎一郎

    高分子   67 ( 6 )   332 - 333   2018年06月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Plasma generated in culture medium induces damages of HeLa cells due to flow phenomena 査読有り

    Sato Y., Sato T., Yoshino D.

    Journal of Physics D: Applied Physics   51 ( 12 )   2018年03月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    Plasma in a liquid has been anticipated as an effective tool for medical applications, however, few reports have described cellular responses to plasma generated in a liquid similar to biological fluids. Herein we report the effects of plasma generated in a culture medium on HeLa cells. The plasma in the culture medium produced not only heat, shock waves, and reactive chemical species but also a jet flow with sub millimeter-sized bubbles. Cells exposed to the plasma exhibited detachment, morphological changes, and changes in the actin cytoskeletal structure. The experimental results suggest that wall shear stress over 160 Pa was generated on the surface of the cells by the plasma. It is one of the main factors that cause those cellular responses. We believe that our findings would provide valuable insight into advancements in medical applications of plasma in a liquid.

    DOI: 10.1088/1361-6463/aab058

    Scopus

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  • Non natural tiny-wet machine: Molecular robot 査読有り

    Yusuke S., Yuichi H., Ibuki K., Satoshi M., Shin-Ichiro N.M.

    MHS 2017 - 28th 2017 International Symposium on Micro-NanoMechatronics and Human Science   2018-January   1 - 3   2018年02月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    The realization of a tiny-wet machine assembled from the molecular devices such as sensors, actuators, and even logic circuits is one of the major goals of the bioinspired micro-nano robotics. We constructed an ameba-like molecular robot capable of expressing continuous shape change in response to specific signal molecules [1]. The robot includes a main body, an actuator, and an actuator control device (molecular clutch). When the clutch was engaged, the robot showed continuous shape change. When the clutch was disconnected, the shape change behavior stopped. These results show that the components of the robot are consistently integrated into the functional system. We are expecting that this research can provide a platform for constructing increasingly complex and functional molecular systems with controllable motility.

    DOI: 10.1109/MHS.2017.8305293

    Scopus

    その他リンク: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050463607&origin=inward

  • アメーバ型分子ロボットの可能性 招待有り

    佐藤佑介, 川又生吹, 野村慎一郎

    医療と検査機器・試薬   40 ( 3 )   190 - 194   2017年06月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

  • Micrometer-sized molecular robot changes its shape in response to signal molecules 査読有り

    Sato Y., Hiratsuka Y., Kawamata I., Murata S., Nomura S.I.M.

    Science Robotics   2 ( 4 )   2017年03月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    Rapid progress in nanoscale bioengineering has allowed for the design of biomolecular devices that act as sensors, actuators, and even logic circuits. Realization of micrometer-sized robots assembled from these components is one of the ultimate goals of bioinspired robotics. We constructed an amoeba-like molecular robot that can express continuous shape change in response to specific signal molecules. The robot is composed of a body, an actuator, and an actuator-controlling device (clutch). The body is a vesicle made from a lipid bilayer, and the actuator consists of proteins, kinesin, and microtubules. We made the clutch using designed DNA molecules. It transmits the force generated by the motor to the membrane, in response to a signal molecule composed of another sequence-designed DNA with chemical modifications. When the clutch was engaged, the robot exhibited continuous shape change. After the robot was illuminated with light to trigger the release of the signal molecule, the clutch was disengaged, and consequently, the shape-changing behavior was successfully terminated. In addition, the reverse process-that is, initiation of shape change by input of a signal-was also demonstrated. These results show that the components of the robot were consistently integrated into a functional system. We expect that this study can provide a platform to build increasingly complex and functional molecular systems with controllable motility.

    DOI: 10.1126/scirobotics.aal3735

    Scopus

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  • Characteristics of plasma in culture medium generated by positive pulse voltage and effects of organic compounds on its characteristics 査読有り

    Sato Y., Sato T., Yoshino D.

    Plasma Sources Science and Technology   25 ( 6 )   2016年11月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    We describe a positive pulse voltage method for generating plasma in culture medium with a composition similar to biological fluids. We also describe the plasma's characteristics, liquid quality, and the effect of organic compounds in the culture medium on the plasma characteristics through comparisons to a solution containing inorganic salts at the same concentrations as in the culture medium. Light emission with Na and OH spectra was observed within a vapor bubble produced by Joule heating at the tip of the electrode. A downward thermal flow and shock wave were caused by the behavior of the vapor bubble. The culture medium pH gradually increased from 7.9 to 8.3 over the discharge time of 300 s. H2O2 was generated 1.1 mg l-1 in the culture medium after discharge for 300 s, and this value was 0.5 mg l-1 lower than the inorganic salts solution which does not contain organic compounds. This study provides important data that will help facilitate more widespread application of plasma medicine.

    DOI: 10.1088/0963-0252/25/6/065023

    Scopus

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  • BIOMOD:分子ロボティクスの次世代育成大会 招待有り

    佐藤 佑介, 瀧ノ上 正浩, 野村 M.慎一郎

    生物物理   2016年08月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(学術雑誌)

▼全件表示

著書

  • Molecular Robotics, An Introduction, Satoshi Murata, Ed.

    Yusuke Sato(分担執筆 ,  範囲: Chapter 3.2 Amoeba-Type Molecular Robot Prototype)

    Springer  2022年08月  ( ISBN:9789811939877

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    総ページ数:296   担当ページ:69-74   記述言語:英語

  • 相分離生物学の全貌(白木賢太郎 編)

    佐藤佑介, 瀧ノ上正浩 (担当:分担執筆, 範囲:49.DNAナノテクノロジーと相分離)(分担執筆)

    東京化学同人  2020年11月 

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    記述言語:日本語

  • 分子ロボティクス概論(村田智 編)

    佐藤 佑介 (担当:分担執筆, 範囲:第2章分子ロボットのシステム化技術 2.2 アメーバ型分子ロボットプロトタイプ)(分担執筆)

    CBI学会出版  2019年05月 

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    記述言語:日本語

口頭発表・ポスター発表等

  • Construction of bio-inspired artificial molecular systems and devices based on DNA nanotechnology 招待有り

    Yusuke Sato

    The 6th FRIS and TFC Collaboration Event “Workshop on Biosystems Design –From nanotechnology to microfluidics in biotechnology” 

     詳細を見る

    開催期間: 2022年12月08日   記述言語:英語  

  • 相分離DNAカプセルを用いた脂質膜の相分離制御に向けて

    柴田航希, 佐藤佑介

    第6回分子ロボティクス年次大会  

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    開催期間: 2022年11月12日 - 2022年11月13日   記述言語:日本語  

  • 脂質膜を貫通し自己集合したDNAによる人工ナノポア構築

    嶋田元徳, 佐藤佑介

    第6回分子ロボティクス年次大会  

     詳細を見る

    開催期間: 2022年11月12日 - 2022年11月13日   記述言語:日本語  

  • DNAナノテクノロジーを用いた疎水分子相互作用解析技術の開発

    楠壱晴, Seaim Lwin Aye, 佐藤佑介

    第6回分子ロボティクス年次大会  

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    開催期間: 2022年11月12日 - 2022年11月13日   記述言語:日本語  

  • 脂質膜を貫通したDNAトランスデューサの構築

    吉海智喜, 佐藤佑介

    第6回分子ロボティクス年次大会  

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    開催期間: 2022年11月12日 - 2022年11月13日   記述言語:日本語  

  • Molecular Dynamics Study of Ion Transport Properties in Artificial DNA Channels

    Jun Takahashi, Akihiro Nakamura, Ibuki Kawamata, Yusuke Sato, Takashi Tokumasu, Takuya Mabuchi

    CBI学会2022年大会 

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    開催期間: 2022年10月25日 - 2022年10月27日   記述言語:日本語  

  • DNAナノテクノロジーを基盤とした疎水分子相互作用研究技術の開発

    楠壱晴, Seaim Lwin Aye, 佐藤佑介

    「細胞を創る」研究会15.0  

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    開催期間: 2022年10月17日 - 2022年10月18日   記述言語:日本語  

  • 脂質膜を貫通したDNAの自己集合による人工ナノポア構築

    嶋田元徳, 佐藤佑介

    「細胞を創る」研究会15.0  

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    開催期間: 2022年10月17日 - 2022年10月18日   記述言語:日本語  

  • Toward the construction of cell-like molecular systems based on DNA nanotechnology ~Engineering by a bottom-up approach~  招待有り

    佐藤佑介

    第3回BVA合成生物学研究会 

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    開催期間: 2022年10月07日   記述言語:日本語  

  • Toward the construction of cell-like molecular systems based on DNA nanotechnology 招待有り

    Yusuke Sato

    第60回生物物理学会年会 

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    開催期間: 2022年09月28日 - 2022年09月30日   記述言語:英語  

  • 疎水分子の自在配置を可能にする新規技術基盤の創出

    佐藤佑介, 楠壱晴, Seaim Lwin Aye

    学術変革領域超越分子システム第2回領域会議 

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    開催期間: 2022年09月25日 - 2022年09月27日   記述言語:日本語  

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講演

  • Assembly and Applications of Liquid-like Condensates Formed from Sequence-Designed DNA Nanostructures

    The 29th International Conference on DNA Computing and Molecular Programming   2023年09月 

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    開催期間: 2023年09月11日 - 2023年09月15日   発表言語:英語   講演種別:基調講演  

  • DNAナノテクノロジーの現状や今後の展望について

    アステラス製薬株式会社 社内研修会  2023年03月 

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    発表言語:日本語   講演種別:招待講演  

学術関係受賞

  • 手島精一記念研究賞(研究論文賞)

    東京工業大学   2023年03月

    瀧ノ上正浩,佐藤佑介,阪本哲郎

     詳細を見る

    受賞国:日本国

  • Chemical and Biological Microsystems Society (CBMS) Researcher Grant

    The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2019)   2019年09月

    Yusuke Sato

     詳細を見る

    受賞国:スイス連邦

  • 優秀研究賞

    化学とマイクロ・ナノシステム学会第38回研究会   2018年11月

    佐藤佑介

     詳細を見る

    受賞国:日本国

  • 若手招待講演賞

    第56回生物物理学会年会   2018年09月

    佐藤佑介

     詳細を見る

    受賞国:日本国

  • 若手研究奨励賞

    分子ロボティクス年次大会   2018年03月

    佐藤佑介

     詳細を見る

    受賞国:日本国

  • 学生発表賞

    第54回生物物理学会年次大会   2016年11月

    佐藤佑介

     詳細を見る

    受賞国:日本国

  • Student Travel Award

    The 22nd International Conference on DNA computing and Molecular Programming   2016年09月

    Yusuke Sato

     詳細を見る

    受賞国:ドイツ連邦共和国

  • 優秀論文賞

    静電気学会   2014年09月

    佐藤佑介

     詳細を見る

    受賞国:日本国

  • エクセレントプレゼンテーションアワード

    静電気学会   2014年09月

    佐藤佑介

     詳細を見る

    受賞国:日本国

▼全件表示

科研費獲得実績

  • 外部刺激により形成・消失を制御可能な人工オルガネラの創成と分子ロボットへの実装

    研究課題番号:20K19918  2020年04月 - 2022年03月   若手研究

  • 分子濃度場を検知し移動する,走化性機能を実装した細胞型分子ロボットの創出

    研究課題番号:18J00720  2018年04月 - 2021年03月   特別研究員奨励費

  • DNA配列設計によりアメーバ運動を自律的に制御する細胞サイズ分子ロボット

    研究課題番号:16J02406  2016年04月 - 2018年03月   特別研究員奨励費

受託研究・共同研究実施実績

  • 自然科学研究機構 自然科学研究機構(NICA)フェロー制度

    2020年08月 - 2021年03月

     詳細を見る

    研究区分:受託研究

担当授業科目(学内)

  • 2023年度   システム回帰分析特論AM

  • 2023年度   システム回帰分析特論SC

  • 2023年度   システム回帰分析特論RO

  • 2023年度   システム制御演習

  • 2023年度   ダイナミクス

  • 2023年度   知的システム工学実験演習Ⅰ

▼全件表示

教育活動に関する受賞・指導学生の受賞など

  • Best Website Award: 2nd Place

    BIOMOD2023 committee    

    2023年11月23日

    YOKABIO

  • Project Award: Gold

    BIOMOD2023 committee    

    2023年11月23日

    YOKABIO

  • Molecular Robotics Award

    分子ロボティクス研究会    

    2023年11月23日

    YOKABIO

  • Chem-Bio Informatics Society学会2023年大会 Student Travel Award

    CBI学会  

    2023年10月23日

    甲斐省吾(指導学生)

  • 第6回分子ロボティクス年次大会 若手研究奨励賞

    計測自動制御学会知能分子ロボティクス研究会  

    2022年11月22日

    柴田航希

その他教育活動

  • 分子ロボティクス夏の学校 2023 講義3 分子システムデザインのためのソフトウェア

    2023年07月
  • 分子ロボティクス夏の学校 2022 講義3 分子システムデザインのためのソフトウェア群

    2022年07月

学会・委員会等活動

  • 生物物理学会   分野別専門委員  

    2024年01月 - 2024年12月

  • International Biomolecular Design Competition, Steering Committee   Committee member  

    2023年04月 - 現在

  • 生物物理学会サブグループ 人工細胞&分子ロボティクス   代表  

    2022年04月 - 2023年03月

  • BIOMOD JAPAN OPEN 2022   実行委員 副委員長  

    2022年04月 - 2022年09月

  • BIOMOD JAPAN OPEN 2021   実行委員 講義・講習担当  

    2021年04月 - 2021年09月

  • BIOMOD JAPAN OPEN 2020   実行委員 講義・講習担当  

    2020年04月 - 2020年09月

  • 生物物理学会   分野別専門委員  

    2020年01月 - 2021年12月

  • 計測自動制御学会 知能分子ロボティクス調査研究会   幹事  

    2019年04月 - 2021年03月

  • CBI学会年次大会   プログラム委員  

    2019年01月 - 現在

▼全件表示

社会貢献活動(講演会・出前講義等)

  • 第73回サイエンスカフェ

    役割:講師

    2024年02月10日

     詳細を見る

    種別:サイエンスカフェ

国際会議開催(学会主催除く)

  • BIOMOD 2023

    分子ロボティクス研究会,BIOMOD Foundation  2023年11月04日 - 2023年11月05日