記述言語：英語   掲載種別：研究論文（学術雑誌）

Anaerobic digestion (AD) of corn straw (CS) inoculated with waste sewage sludge (WSS) is a promising approach for energy utilization of both lignocellulosic wastes and WSS. However, few studies about the effect of antibiotics contained in WSS on AD of CS are reported, which hinders the efficient conversion from CS to methane (CH4). Antibiotics including tetracycline (TC) and azithromycin (AZM) were used to evaluate their effects on AD of CS inoculated with WSS in this study. As results, the reactor with AZM addition achieved 167.67 mL/g VS CH4 yield, which was increased 65.22% compared with CTRL (without any antibiotics addition, 101.48 mL/g VS CH4 yield) by playing roles at the hydrolysis, acidogenesis/acetogenesis and methanogenesis stages during 20 days of AD. Similarly, addition of TC performed 25.38% (127.27 mL/g VS) higher CH4 yield than CTRL mainly by promoting the acidogenesis/acetogenesis and methanogenesis stages. Specifically, phyla Bacteroidetes, Actinobacteria and genera Methanosarcina, Methanobrevibacter were considered as functional microorganisms for AD of CS with AZM addition. Results from this study provide a new insight for enhancing CH4 yield from CS.

DOI： 10.1016/j.bcab.2023.102732

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Meat products are commonly associated with foodborne diseases outbreak. Bacteria have been long used as a hygienic indicator in the food industry. Food-contacted surfaces could be the source of contamination causing foodborne illness, particularly when involving raw meats. Hence, improving knowledge about bacteria that remains on meat-contacted surfaces can be influential in determining appropriate guidelines for food safety. This study aimed firstly to determine bacterial community structure and diversity. Secondly, to determine taxa co-occurrence network and differentially abundant bacteria and finally, to predict the functional profiles of bacterial communities residing on the food-associated surfaces used in industrial food preparation areas, i.e., aluminum tray, following contact with beef, chicken, and pork using 16S rRNA amplicon metagenomics on the Illumina MiSeq system. The most abundant bacteria on meat-contacted surfaces were determined, and Acinetobacter and Pseudomonas were among the dominant bacteria in all meat types. Chicken-contacted trays showed the highest species richness, followed by pork and beef, with a significant difference. The chicken-contacted tray bacterial community was significantly different from other groups. The co-occurrence network was predicted, and hub nodes were determined. Low-abundance bacteria have been proven to have a significant function in networks. Some of the hub nodes were potential human pathogens or antibiotic-resistant. In addition, bacterial markers were determined; some were human pathogens, such as Shewanella in chicken, Staphylococcus in beef, and Klebsiella in pork. Although the predicted metagenomic functions were not significantly different, some were related to human diseases and resistance to antibiotics or disinfectants. In conclusion, bacterial pathogens were found on aluminum trays despite standard storage practices, demonstrating the need for proper hygiene instructions and regular cleaning of food preparation facilities and equipment to prevent cross-contamination. This study detailed bacterial taxa on meat-contacted trays, and the findings are critical to improving foodborne pathogen decontamination and developing safer food handling guidelines and standards. Radiation, heat, and chemical approaches are said to be influential in preventing contamination.

DOI： 10.1016/j.foodcont.2023.109698

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記述言語：英語   掲載種別：研究論文（学術雑誌）

A current Microbial Desalination Cell (MDC) system often uses chemicals or air cathodes that are toxic and impractical for scalable applications. This work presents the MDC performance that utilized microalgae species Chlamydomonas sp. (UKM6) and Scenedesmus sp. (UKM9). These species supported the role of the terminal electron acceptor in the cathode chamber of photosynthetic MDCs (PhMDC). The results showed that PhMDC-UKM9 and UKM6 produced 1942 mW/m3 and 1714 mW/m3 power densities with 44% and 32% desalination rates, respectively. The desalination of salt concentration (35 g/L) was approaching the practical application of seawater. Both UKM6 and UKM9 achieved chemical oxygen demand (COD) removal in the anode chamber by 49% and 53%, respectively. 16S microbial community analysis in anolyte revealed phylum Firmicutes as the dominion community. This study demonstrated that the local microalgae species integrate power production, wastewater treatment, and water purification through PhMDC operation, comparable with other studies using commercial microalgae.

DOI： 10.1016/j.ijhydene.2023.01.142

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Waterborne diseases due to pathogen contamination in water are a serious problem all over the world. Accurate and simultaneous detection of pathogens in water is important to protect public health. In this study, we developed a method to simultaneously detect various pathogenic Escherichia coli by sequencing the amplicons of multiplex PCR. Our newly designed multiplex PCR amplified five genes for pathogenic E. coli (uidA, stx1, stx2, STh gene, and LT gene). Additional two PCR assays (for aggR and eae) were also designed and included in the amplicon sequencing analysis. The same assays were also used for digital PCR (dPCR). Strong positive correlations were observed between the sequence read count and the dPCR results for most of the genes targeted, suggesting that our multiplex PCR-amplicon sequencing approach could provide quantitative information. The method was also successfully applied to monitor the level of pathogenic E. coli in river water and wastewater samples. The approach shown here could be expanded by targeting genes for other pathogens.

DOI： 10.1007/s10661-022-10863-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）

The incorporation of antimicrobial peptides (AMPs) in graphene-based materials is a growing strategy to overcome the shortcomings presented by conventional antimicrobial nanomaterials derived from metal/metal oxide. In this study, novel cyclic undecapeptides CH1 and CW2 (KRQRFYFRQRK and QNKRFYFRKNQ, respectively) were successfully synthesized. The cyclic peptides were then immobilized on the reduced graphene oxide (rGO) surface using a water-soluble carbodiimide (EDCI/HCl) linker. The structural, morphology, and chemical characterizations of the antimicrobial graphene material were carried out using SEM, Raman, FTIR, XRD, and XPS. From our result, the peptide/rGO composite showed an improved antibacterial effect towards gram-positive (Staphylococcus aureus) and gram-negative (Pseudomonas aeruginosa). The enhanced antibacterial property is attributed to the synergistic effect of the sharp edges of rGO and the amphipathic moieties of the cyclic peptides.

DOI： 10.1007/s13762-023-05035-z

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記述言語：英語   掲載種別：研究論文（学術雑誌）

We report the complete genome of Paenibacillus sp. strain VCA1, which was isolated from sediment from El Chichón Volcano. This genome consists of 6,690,819 bp and 6,312 coding sequences, with 51.8% G+C content. Whole-genome sequencing was performed to explore the strain's biocontrol and plant growth promotion properties.

DOI： 10.1128/MRA.00583-23

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Background: Bacteriophage therapy is becoming part of mainstream Western medicine since antibiotics of clinical use tend to fail. It involves applying lytic bacteriophages that self-replicate and induce cell lysis, thus killing their hosts. Nevertheless, bacterial killing promotes the selection of resistant clones which sometimes may exhibit a decrease in bacterial virulence or antibiotic resistance. Methods: In this work, we studied the Pseudomonas aeruginosa lytic phage φDCL-PA6 and its variant φDCL-PA6α. Additionally, we characterized and evaluated the production of virulence factors and the virulence in a Galleria mellonella model of resistant mutants against each phage for PA14 and two clinical strains. Results: Phage φDCL-PA6α differs from the original by only two amino acids: one in the baseplate wedge subunit and another in the tail fiber protein. According to genomic data and cross-resistance experiments, these changes may promote the change of the phage receptor from the O-antigen to the core lipopolysaccharide. Interestingly, the host range of the two phages differs as determined against the Pseudomonas aeruginosa reference strains PA14 and PAO1 and against nine multidrug-resistant isolates from ventilator associated pneumonia. Conclusions: We show as well that phage resistance impacts virulence factor production. Specifically, phage resistance led to decreased biofilm formation, swarming, and type III secretion; therefore, the virulence towards Galleria mellonella was dramatically attenuated. Furthermore, antibiotic resistance decreased for one clinical strain. Our study highlights important potential advantages of phage therapy’s evolutionary impact that may be exploited to generate robust therapy schemes.

DOI： 10.3389/fcimb.2023.1280265

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記述言語：英語   掲載種別：研究論文（学術雑誌）

The bacterial archetypal adaptive immune system, CRISPR-Cas, is thought to be repressed in the best-studied bacterium, Escherichia coli K-12. We show here that the E. coli CRISPR-Cas system is active and serves to inhibit its nine defective (i.e., cryptic) prophages. Specifically, compared to the wild-type strain, reducing the amounts of specific interfering RNAs (crRNA) decreases growth by 40%, increases cell death by 700%, and prevents persister cell resuscitation. Similar results were obtained by inactivating CRISPR-Cas by deleting the entire 13 spacer region (CRISPR array); hence, CRISPR-Cas serves to inhibit the remaining deleterious effects of these cryptic prophages, most likely through CRISPR array-derived crRNA binding to cryptic prophage mRNA rather than through cleavage of cryptic prophage DNA, i.e., self-targeting. Consistently, four of the 13 E. coli spacers contain complementary regions to the mRNA sequences of seven cryptic prophages, and inactivation of CRISPR-Cas increases the level of mRNA for lysis protein YdfD of cryptic prophage Qin and lysis protein RzoD of cryptic prophage DLP-12. In addition, lysis is clearly seen via transmission electron microscopy when the whole CRISPR-Cas array is deleted, and eliminating spacer #12, which encodes crRNA with complementary regions for DLP-12 (including rzoD), Rac, Qin (including ydfD), and CP4-57 cryptic prophages, also results in growth inhibition and cell lysis. Therefore, we report the novel results that (i) CRISPR-Cas is active in E. coli and (ii) CRISPR-Cas is used to tame cryptic prophages, likely through RNAi, i.e., unlike with active lysogens, active CRISPR-Cas and cryptic prophages may stably co-exist.

DOI： 10.3390/ijms232416195

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担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

Although Escherichia coli has four hydrogenases, their definite roles in fermentation are still not clear. In this study, all the operon deletion mutants of E.coli hydrogenases (∆hya, ∆hyb, ∆hyc, or ∆hyf) were constructed to evaluate the hydrogen metabolism in comparison to their respective single-gene deletion mutants of large subunits (∆hyaB, ∆hybC, ∆hycE, and ∆hyfG). Besides the hyc operon mutant that expectedly showed no hydrogen synthesis, the hyb operon mutant showed low hydrogen production and demonstrated significantly reduced growth under anaerobic conditions. The present work also provided first-hand data where deleterious effects of operon deletion were compared with single-gene deletion mutations and the results showed that the former type of deletion was found to cause more prominent phenotypic effects than the latter one. Interestingly, hyb operon mutant was remarkably distinct from other operon mutants, specifically in its inability to utilize glucose under both aerobic and anaerobic conditions. Further studies on this mutant revealed a significant reduction of the total intracellular ATP and NADH concentrations, which could explain its impaired glucose metabolism. In this way, Hyd-2 was verified as crucial not only in glucose metabolism but also in energy balance and redox homeostasis of the cells. Furthermore, a decreased expression of glucose metabolism-associated genes, particularly ppc and pykA, indicated their regulation by hyb operon, and thereby, glucose consumption. Moreover, the transcriptional changes in this mutant indicated the wide genomic connectivity of hyb operon to other metabolisms.

DOI： 10.1007/s00203-022-03245-6

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記述言語：英語   掲載種別：研究論文（学術雑誌）

A challenge in the study of gastrointestinal microbiota (GITm) is the validation of the genomic data with metabolic studies of the microbial communities to understand how the microbial networks work during health and sickness. To gain insights into the metabolism of the GITm, feces from healthy and sick rats with cancer were inoculated in a defined synthetic medium directed for anaerobic prokaryote growth (INC-07 medium). Significant differences between cultures of healthy and sick individuals were found: 1) the consumption of the carbon source and the enzyme activity involved in their catabolism (e.g., sucrase, lactase, lipases, aminotransferases, and dehydrogenases); 2) higher excretion of acetic, propionic, isobutyric, butyric, valeric, and isovaleric acids; 3) methane production; 4) ability to form biofilms; and 5) up to 500 amplicon sequencing variants (ASVs) identified showed different diversity and abundance. Moreover, the bowel inflammation induced by cancer triggered oxidative stress, which correlated with deficient antioxidant machinery (e.g., NADPH-producing enzymes) determined in the GITm cultures from sick individuals in comparison with those from control individuals. Altogether, the data suggested that to preserve the microbial network between bacteria and methanogenic archaea, a complete oxidation of the carbon source may be essential for healthy microbiota. The correlation of 16S rRNA gene metabarcoding between cultures and feces, as well as metabolomic data found in cultures, suggest that INC-07 medium may be a useful tool to understand the metabolism of microbiota under gut conditions.

DOI： 10.3389/fmicb.2022.949272

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担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1002/jobm.202100636

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

The effect of sodium selenite on anaerobic digestion of waste sewage sludge (WSS) was studied. Interestingly, methane production was enhanced at 2 mg/L and inhibited at 100 mg/L of sodium selenite during the anaerobic digestion of WSS. Hydrolytic activity increased in correlation with increased concentrations of sodium selenite (0 to 100 mg/L). At the acidogenesis stage, acetic acid accumulated at the concentrations more than 25 mg/L of sodium selenite whereas it was consumed for 10 days at the concentration of 2 mg/L of sodium selenite. Also, the concentration of propionic acid decreased and that of butyric acid increased at 100 mg/L of sodium selenite. Although methanogenic activities were totally inhibited at 100 mg/L of sodium selenite, a high hydrogenotrophic methanogenic activity was observed at 2 mg/L of sodium selenite. The abundance of hydrogenotrophic methanogens (Methanospirillum and Methanocorpusculum) increased in the WSS at 2 mg/L of sodium selenite. Moreover, the results of pure-culture experiments using Methanosarcina acetivorans C2A show that the growth promotion or inhibition was observed at 2 mg/L or at 100 mg/L of sodium selenite. In conclusion, sodium selenite has the double-sided effect during the anaerobic digestion of WSS as shown by the results that methane production was promoted at a low concentration and inhibited at a high concentration.

DOI： 10.1016/j.eti.2022.102403

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記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1264/jsme2.ME22004

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

The role of bacterial interaction is vital to control bacterial functions; however, it has not been fully understood in microbial consortia (including anaerobic digestion). In this study, fluorouracil (FU), which is an anticancer agent and a quorum sensing (QS) inhibitor to some of the Gram-negative bacteria was found to inhibit methane production from sewage sludge under anaerobic conditions, as shown in a result where methane production in the presence of FU was eight times lower than the control (sewage sludge without FU). Whereas FU did not influence the hydrolysis process, in the acidogenesis/acetogenesis process, butyrate, and acetate accumulated in samples with FU. Also, in the methanogenesis process, FU remarkably inhibited methane production by acetoclastic methanogens rather than by the hydrogenotrophic ones. This result agreed with the result that growth and methane production of Methanosarcina acetivorans C2A was inhibited in the presence of FU. However, the inhibitory effect of FU was high in the condition that both bacteria and archaea were active. It indicates that FU influences methanogens and bacteria in the process of methane fermentation. The analyses of microbial communities (bacteria and archaea) showed that the abundance ratio of the Firmicutes phyla is high, and hydrogenotrophic methanogens become dominant in the presence of FU. Conversely, the abundance of Spirochaetes significantly decreased under FU. The inhibition of methane production by FU was due to the growth inhibition of methanogenic archaea and the changes in the composition of the bacterial population.

DOI： 10.1016/j.chemosphere.2022.134253

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

In this study, the effect of sodium tungstate on anaerobic digestion of waste sewage sludge (WSS) was investigated. Methane production was enhanced by sodium tungstate in a dose-dependent manner. Sodium tungstate did not affect the hydrolysis stage as the same level of protease activity was detected in the WSS sample with or without sodium tungstate. At the acidogenesis stage, the concentration of acetate decreased during the anaerobic digestion of WSS supplemented with sodium tungstate whereas that of isobutyrate increased. The pH of WSS increased in the presence of sodium tungstate, correlating with the result of acetate consumption with time during the anaerobic digestion of WSS. The analyses of methanogenesis via acetoclastic and hydrogenotrophic methanogens indicate that methane production from acetate was high in the WSS with sodium tungstate. In addition, the addition of sodium tungstate to the initial fresh WSS did not enhance methane production from acetate; therefore, sodium tungstate may increase the number of acetoclastic methanogens rather than promote the catalytic reaction related to the methane production. The analyses of archaeal population supported that acetoclastic methanogens (mostly Methanosaeta) increased with time in the WSS with sodium tungstate. In detail, when compared to the control WSS, the percentage of Methanosaeta significantly increased from 3.02% to 31.20% in the presence of sodium tungstate. Furthermore, the addition of sodium tungstate enhanced the growth of Methanosarcina acetivorans C2A. Taken together, sodium tungstate increases methane production via the anaerobic digestion of WSS by increasing the activity of acetoclastic methanogenesis.

DOI： 10.1016/j.jece.2022.107524

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担当区分：筆頭著者,　最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）

DOI： 10.34565/seibutsukogaku.100.4_210

CiNii Research

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.3389/fbioe.2022.894553

担当区分：最終著者   記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）

While chemical fertilisers and pesticides indeed enhance agricultural productivity, their excessive usage has been detrimental to environmental health. In addressing this matter, the use of environmental microbiomes has been greatly favoured as a ‘greener’ alternative to these inorganic chemicals’ application. Challenged by a significant proportion of unidentified microbiomes with unknown ecological functions, advanced high throughput metatranscriptomics is prudent to over-come the technological limitations in unfolding the previously undiscovered functional profiles of the beneficial microbiomes. Under this context, this review begins by summarising (1) the evolution of next-generation sequencing and metatranscriptomics in leveraging the microbiome transcriptome profiles through whole gene expression profiling. Next, the current environmental metatranscrip-tomics studies are reviewed, with the discussion centred on (2) the emerging application of the beneficial microbiomes in developing fertile soils and (3) the development of disease-suppressive soils as greener alternatives against biotic stress. As sustainable agriculture focuses not only on crop productivity but also long-term environmental sustainability, the second half of the review highlights the metatranscriptomics’ contribution in (4) revolutionising the pollution monitoring systems via specific bioindicators. Overall, growing knowledge on the complex microbiome functional profiles is imperative to unlock the unlimited potential of agricultural microbiome-based practices, which we believe hold the key to productive agriculture and sustainable environment.

DOI： 10.3390/ijms23073737

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担当区分：最終著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）

<p>Nucleic acid extracted from environmental samples is an important analyte in instrumental analysis. With respect to the phenomenon in which sodium tungstate promotes the methane production in anaerobic digestion, the analyses of microbial community using RNA were conducted; however, we noticed that only RNA concentration measured by absorbance measurement (NanoDrop) was remarkably high. Therefore, in this study, DNA and RNA were extracted from the anaerobic digestion sludge samples with sodium tungstate, sodium selenite, or sodium molybdate, and these nucleic acids were quantified and compared by absorbance measurement, fluorescence measurement (Qubit), and gel electrophoresis. Interestingly, it was found that only the RNA concentration of the sample containing sodium tungstate measured by NanoDrop was 3 times higher than that by Qubit analysis. In addition, there was no difference between the RNA concentration measured by Qubit and gel electrophoresis. Regarding DNA concentration and the other compounds, there were no differences. Hence, these results indicate that the Qubit system is useful for the quantification of the RNA concentration in the environmental samples.</p>

DOI： 10.2116/bunsekikagaku.71.201

CiNii Research

記述言語：日本語   掲載種別：記事・総説・解説・論説等（学術雑誌）

<p>With the recent increase in demand for biodegradable plastics triggered by the microplastics problem, the reuse of waste is also attracting attention. For example, VTT Technical Research Centre of Finland holds a patent to produce high-purity oligo(lactic acid) (OLA) from polylactic acid waste. If a new useful strain capable of assimilating the OLA can be discovered, it will be possible to produce useful substances from polylactic acid-based waste. For that purpose, it is necessary to clarify the relationship between bacteria and the OLA. As part of this, we tried to analyze the hydrolysis behavior of oligo(lactic acid) using reverse-phase HPLC. From the results, it was shown that the addition of trifluoroacetic acid (TFA) to the HPLC eluent significantly suppressed the broadening of the peaks derived from the OLA, enabling the quantification of OLA. The analytical method will be useful for investigating the effects of useful bacteria on the OLA.</p>

DOI： 10.2116/bunsekikagaku.71.197

CiNii Research

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.chemosphere.2021.131731

記述言語：英語   掲載種別：研究論文（学術雑誌）

Herein, we prepared and characterized two antimicrobial cyclic peptides immobilized on reduced graphene oxide (rGO) and accessed their anti-bacterial towards Gram-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus) bacterias. The covalent interaction between the tyrosine and histidine based cyclic peptides and the rGO occurred via the carboxylic groups of the reduced graphene and the amino side chain groups of the cyclic peptides. A selective antibacterial effect is observed for the cyclic peptides functionalized rGO. The inhibition zone test showed that both the tyrosine and histidine cyclic based peptide immobilized on rGO exhibited higher anti-bacterial potency towards the bacteria in comparison to bare rGO which can be attributed to the synergistic effect of the antimicrobial peptide and rGO.

DOI： 10.1016/j.matlet.2021.130621

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Zero-emission technology for palm oil mill effluent (POME) has led to a breakthrough in the palm oil industry in relation to the goal of sustainable development. However, there are limited resources on how this technology has affected the bacterial community in the receiving river that has previously been polluted with POME final discharge. Thus, the current study assessed the recoverability of the unexplored bacterial community in the receiving water of a constructed river water system post-zero emission of POME final discharge. An artificial river water system was constructed in this study, where the viability status and the composition of the bacterial community were assessed for 15 days using a flow cytometry-based assay and high-throughput sequencing by Illumina MiSeq, respectively. The zero-emission of POME final discharge reduced not only the physicochemical properties and nutrient contents of the receiving water, but also the bacterial cells’ viability from 40.3% to 24.5% and shifted the high nucleic acid (HNA) to low nucleic acid (LNA) content (38.7% to 34.5%). The proposed POME bacterial indicators, Alcaligenaceae and Chromatiaceae were not detectable in the rainwater (control) but were detected in the artificial river water system after the introduction of POME final discharge at the compositions of 1.0–1.3% and 2.2–5.1%, respectively. The implementation of a zero-emission system decreased the composition of Chromatiaceae from 2.2% on day 8 until it was undetectable on day 15, while Alcaligenaceae was continuously reduced from 1.2% to 0.9% within that similar time frame. As indicated by principal coordinate (PCO) analysis, the reductions in biological oxygen demand (BOD5 ) would further diminish the compositions of these bioindicators. The zero-emission of POME final discharge has demonstrated its efficacy, not only in reducing the polluting properties, but also in the bacterial biodiversity rebound in the affected water system.

DOI： 10.3390/app112210814

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記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）

Dairy manure (DM) is a kind of cheap cellulosic biomass resource which includes lignocellulose and mineral nutrients. Random stacks not only leads damage to the environment, but also results in waste of natural resources. The traditional ways to use DM include returning it to the soil or acting as a fertilizer, which could reduce environmental pollution to some extent. However, the resource utilization rate is not high and socio-economic performance is not utilized. To expand the application of DM, more and more attention has been paid to explore its potential as bioenergy or bio-chemicals production. This article presented a comprehensive review of different types of bioenergy production from DM and provided a general overview for bioenergy production. Importantly, this paper discussed potentials of DM as candidate feedstocks not only for biogas, bioethanol, biohydrogen, microbial fuel cell, lactic acid, and fumaric acid production by microbial technology, but also for bio-oil and biochar production through apyrolysis process. Additionally, the use of manure for replacing freshwater or nutrients for algae cultivation and cellulase production were also discussed. Overall, DM could be a novel suitable material for future biorefinery. Importantly, considerable efforts and further extensive research on overcoming technical bottlenecks like pretreatment, the effective release of fermentable sugars, the absence of robust organisms for fermentation, energy balance, and life cycle assessment should be needed to develop a comprehensive biorefinery model.

DOI： 10.1016/j.scitotenv.2021.148199

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

Bdellovibrio bacteriovorus is one of the predatory bacteria; therefore, it can act as a novel “living antibiotic,” unlike the current antibiotics. Here the predation of Escherichia coli by B. bacteriovorus was inhibited in the presence of Pseudomonas aeruginosa. This study investigated whether P. aeruginosa-induced predation inhibition is associated with bacterial quorum sensing (QS). Each las, rhl, or pqs QS mutant in P. aeruginosa was used to check the predatory activity of E. coli cells using B. bacteriovorus. As a result, the predatory activity of B. bacteriovorus increased in a mutant pqs QS system, whereas wild-type PA14 inhibited the predatory activity. Moreover, the addition of 4-hydroxy-2-heptylquinoline (HHQ) or the analog triggered the low predatory activity of B. bacteriovorus and killed B. bacteriovorus cells. Therefore, a defensive action of P. aeruginosa against B. bacteriovorus is activated by the pqs QS system, which produces some quinolone compounds such as HHQ.

DOI： 10.3389/fmicb.2021.722579

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Pseudomonas aeruginosa RW9 is a promising candidate for the bioremediation of chromium hexavalent (Cr(VI)) pollution, as it resists a high concentration of up to 60 mg/L of Cr(VI). Leaving cells exposed to Cr(VI) has large bioreduction potential, implying its capacity to extract the ions from the contaminated medium. In this study, the tolerance for and distribution of Cr(VI) were investigated to identify the cells’ adaptation and removal strategies. Micro-characterization analysis was conducted to assess the effect of Cr(VI) on the cells. The cells’ elongation was observed at higher Cr(VI) concentrations, signifying their adaptation to DNA damage caused by Cr(VI) toxicity. Cr(VI) distribution analysis showed that the strain developed a complex mechanism to adapt to Cr(VI), based on surface-bound (0.46 mg/L), intracellularly accumulated (1.24 mg/L) and extracellular sequestration (6.74 mg/L), which accounted for 85% of the removal efficiency. The extracellular sequestration might be attributable to the production of metabolites, in accordance with the fourier-transform infrared spectroscopy (FTIR) spectra and orcinol analysis that confirmed the presence of a glycolipid biosurfactant, a rhamnolipid. Remarkably, the rhamnolipid was slightly induced in the presence of Cr(VI). From the data obtained, it was confirmed that this local strain is well equipped to survive high doses of Cr(VI) and has great potential for application in Cr(VI) bioremediation.

DOI： 10.3390/su13179797

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Most palm oil mills adopted conventional ponding system, including anaerobic, aerobic, facultative and algae ponds, for the treatment of palm oil mill effluent (POME). Only a few mills installed a bio-polishing plant to treat POME further before its final discharge. The present study aims to determine the quality and toxicity levels of POME final discharge from three different mills by using conventional chemical analyses and fish (Danio rerio) embryo toxicity (FET) test. The effluent derived from mill A which installed with a bio-polishing plant had lower values of BOD, COD and TSS at 45 mg/L, 104 mg/L, and 27 mg/L, respectively. Only mill A nearly met the industrial effluent discharge standard for BOD. In FET test, effluent from mill A recorded low lethality and most of the embryos were malformed after hatching (half-maximal effective concentration (EC50) = 20%). The highest toxicity was observed from the effluent of mill B and all embryos were coagulated after 24 h in samples greater than 75% of effluent (38% of half-maximal lethal concentration (LC50) at 96 h). The embryos in the effluent from mill C recorded high mortality after hatching, and the survivors were malformed after 96 h exposure (LC50 = 26%). Elemental analysis of POME final discharge samples showed Cu, Zn, and Fe concentrations were in the range of 0.10–0.32 mg/L, 0.01–0.99 mg/L, and 0.94–4.54 mg/L, respectively and all values were below the effluent permissible discharge limits. However, the present study found these metals inhibited D. rerio embryonic development at 0.12 mg/L of Cu, and 4.9 mg/L of Fe for 96 h-EC50. The present study found that bio-polishing plant installed in mill A effectively removing pollutants especially BOD and the FET test was a useful method to monitor quality and toxicity of the POME final discharge samples.

DOI： 10.1016/j.envpol.2021.116780

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Biodiesel side stream waste glycerol was identified as a cheap carbon source for rhamnolipids (RLs) production which at the same time could improve the management of waste. The present study aimed to produce RLs by using Pseudomonas aeruginosa RS6 utilizing waste glycerol as a substrate and to evaluate their physico-chemicals properties. Fermentation conditions such as temperature, initial medium pH, waste glycerol concentration, nitrogen sources and concentrations resulted in different compositions of the mono- and di-RLs produced. The maximum RLs production of 2.73 g/L was obtained when P. aeruginosa RS6 was grown in a basal salt medium supplemented with 1% waste glycerol and 0.2 M sodium nitrate at 35 °C and pH 6.5. At optimal fermentation conditions, the emulsification index (E24) values of cooking oil, diesel oil, benzene, olive oil, petroleum, and kerosene were all above E24=50%. The surface tension reduction obtained from 72.13 mN/m to 29.4–30.4 mN/m was better than the surface activity of some chemical-based surfactants. The RLs produced possessed antimicrobial activities against Gram-negative and Gram-positive bacteria with values ranging from 37% to 77% of growth inhibition when 1 mg/mL of RLs was used. Concentrations of RLs below 1500 μg/mL did not induce phytotoxicity effects on the tested seeds (Vigna radiata) compared to the chemical-based- surfactant, SDS. Furthermore, RLs tested on zebrafish (Danio rerio) embryos only exhibited low acute toxicity with an LC50 value of 72.97 μg/mL at 48 h of exposure suggesting a green and eco-biochemical worthy of future applications to replace chemical-based surfactants. Pseudomonas aeruginosa RS6 shows the ability to consume biodiesel side stream waste glycerol for the production of green biochemicals and less-toxic rhamnolipids.

DOI： 10.1016/j.envpol.2021.116742

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Chitosan nanofiber has a highly uniform structure of 20–50 nm in diameter and shows high dispersibility in water due to its submicron size and high surface-to-volume ratio. The stacked nanofibers film is useful for breathability because it has a gap with a size of several tens of nm or more. However, the chemical bonds between the nanofibers cannot be broken during use. In this study, the thin films were obtained by filtration of chitosan nanofibers and 3-glycidoxypropyltri-methoxysilane (GPTMS) mixture. The addition of GPTMS changed the wettability, mechanical property and stability in water of the thin films. Bacitracin zinc salt (BZ) has been used for the localized dermatological medicines and loaded in the films. BZ interacted electrostatically with the thin films matrix and the release of BZ was controlled by the amount of GPTMS. A higher released amount of BZ showed higher antibacterial effects toward S. aureus. The film was also tested their toxicity by L929 fibroblasts. The release of less than 11.9 μg of BZ showed antibacterial effects, but were not toxic for fibroblast cells.

DOI： 10.3390/polym13071104

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Bacteria have acquired resistance against almost all antibiotics because of the misuse of antibacterial agents and long periods of treatment. Antimicrobial peptides (AMPs) are one of the most encouraging candidates to solve this problem, as they possess high prokaryotic selectivity, and affect the bacteria by a unique mode of action. Novel cyclic undecapeptides (QNRNFYFNRNQ and QNRNFHFNRNQ) and their linear counterparts were investigated for their antibacterial activity against virulent strains. The minimal inhibitory concentration (MIC) values showed that tyrosine and histidine AMPs have promising antibacterial activity against virulent bacteria. The MIC values against the P. aeruginosa PA14, E. coli O157:H7 CR3, S. aureus 209P, and B. subtilis ATCC 6633 bacterial strains were evaluated for the cyclic peptide containing tyrosine, and their values were 6.25, 12.5, 12.5, and 12.5 µM, respectively. Meanwhile, for the linear form, they were 9.3, 12.5, 12.5, and 12.5 µM, respectively. The cyclic-peptide–containing histidines’ MIC values were 6.25, 3.1, 6.25, and 3.1 µM, respectively. Meanwhile, for the linear form, they were 3.1, 3.1, 3.1, and 6.25 µM, respectively. The antibacterial activities of the new AMPs were compared with that of gentamicin sulfate, and showed relatively higher potencies. Time-inhibition studies demonstrated the rapid antibacterial effects of the novel AMPs, which were more likely to be concentration-dependent, rather than time-dependent. At double the MIC concentration, all of the tested peptides exhibited relatively stable antibacterial effects up to 24 h, especially the peptides containing tyrosine, which showed an improved antibacterial effect.

DOI： 10.3390/scipharm89010010

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

Low ability of waste sewage sludge to degrade cellulose is observed due to its less cellulolytic bacteria content. The enrichment of sewage sludge in the absence or presence of carboxymethylcellulose (CMC) was conducted to improve anaerobic digestion (AD) of cellulose in this study. Compared to initial sewage sludge (IS), enriched sludge without CMC addition (ES) displayed 69.81% higher CH yield and about 1.7-fold greater anaerobic biodegradation of cellulose. In particular, bacterial and archaeal diversities in samples inoculated with ES were significantly altered, with Ruminiclostridium and Methanobacterium as the predominant genera. Enriched sludge with CMC addition (ESC) displayed enhanced methane production at initial cellulose fermentation but showed no distinct difference compared with the control after incubation 24 days. These findings suggest that enrichment of waste sewage sludge without CMC addition is more beneficial for promoting AD of cellulose, providing a novel insight for efficient energy utilization of lignocellulosic wastes. 4

DOI： 10.1016/j.biortech.2020.124497

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Aim: As options to treat recalcitrant bacterial infections which are increasingly limited due to multidrug-resistant strains, searching for new, effective antibacterial compounds is necessary. One strategy is to generate treatment alternatives by drug repurposing. Methods and Results: In this work, phenotypic microarrays were used for the screening of miscellaneous compounds against the growth and biofilm formation of Acinetobacter baumannii, an important emergent multidrug-resistant opportunistic pathogen. The results showed that the phenothiazine derivatives, such as promethazine, trifluoperazine, thioridazine, and chlorpromazine, inhibited the growth of antibiotic-sensitive and multidrug-resistant strains (showing minimal inhibitory concentrations ranging from 0·05 to 0·6 g l−1 and minimal bactericidal concentrations ranging from 0·1 to 2·5 g l−1). All phenothiazine derivatives were active against biofilm cells (with minimal biofilm eradication concentrations ranging from 0·5 to >3 g l−1). Chlorpromazine promoted reactive oxigen species (ROS) production, and cell membrane and DNA damage. Chlorpromazine showed synergy with antibiotics such as ceftazidime, meropenem, and colistin and was an effective treatment for experimentally infected Galleria mellonella when combined with ceftazidime. Conclusions: It was demonstrated that phenothiazine derivatives, especially chlorpromazine, are drugs with attractive antibacterial properties against nosocomial MDR strains of A. baumannii, by generating ROS and cell membrane and DNA damage. Significance and Impact of the Study: The present study indicates that repurposing phenothiazine derivatives for treating recalcitrant infections by A. baumannii could be promising.

DOI： 10.1111/jam.15109

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Palm Oil Mill Effluent (POME) requires treatment before disposal due to its high organic matter content. In this study, the electrical performance and wastewater treatment efficiency were evaluated for Microbial Fuel Cells (MFC) treating unsterile POME with chemical oxygen demand (COD) from 200 to 10 000 mg/L. Since the inoculum type is a key factor in MFC performance, three types of sludge (methanogenic sludge (MS), facultative sludge (FS), and dry sludge (DS), obtained from the current POME treatment ponds were evaluated as inoculum. Dry sludge (DS) developed a maximum power output of 3.30 W/m3 by oxidizing 71% out of the COD provided by POME (1000 mg/L). Also, raw POME microbiota contributed to an enrichment of the community in DS inoculum along with the operation, in which Geobacter was the predominant genus reaching a current generation of 247 mA/m2 and a power density of 2.36 W/m3. Conversely, pure electrogenic (Shewanella sp.) inoculation led to a diversification process, resulting in a lower current generation of 52 mA/m2 and a power density of 0.10 W/m3. Consequently, microbial community dynamics revealed that MFC inoculation tends to a microbial equilibrium wherein generation of high current density was achieved by gradual microbial enrichment rather than external electrogenic invasion.

DOI： 10.1016/j.ijhydene.2021.08.218

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担当区分：筆頭著者   記述言語：英語   掲載種別：記事・総説・解説・論説等（学術雑誌）

Abstract: Anaerobic digestion of sewage sludge (SS) is one of the effective ways to reduce the waste generated from human life activities. To date, there are many reports to improve or repress methane production during the anaerobic digestion of SS. In the anaerobic digestion process, many microorganisms work positively or negatively, and as a result of their microbe-to-microbe interaction and regulation, methane production increases or decreases. In other words, understanding the complex control mechanism among the microorganisms and identifying the strains that are key to increase or decrease methane production are important for promoting the advanced production of bioenergy and beneficial compounds. In this mini-review, the literature on methane production in anaerobic digestion has been summarized based on the results of antibiotic addition, quorum sensing control, and inorganic substance addition. By optimizing the activity of microbial groups in SS, methane or acetate can be highly produced. Key points: • Bactericidal agents such as an antibiotic alter microbial community for enhanced CH4production. • Bacterial interaction via quorum sensing is one of the key points for biofilm and methane production. • Anaerobic digestion can be altered in the presence of several inorganic materials.

DOI： 10.1007/s00253-021-11536-z

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記述言語：英語   掲載種別：研究論文（学術雑誌）

<p>A novel deep-branching archaeal lineage was discovered at high-temperature hot springs around Oyasukyo Gorge in Akita Prefecture, Japan. Actively boiling hot spring water contained >1×10⁴ microbes mL^–1. The microbial community composition assessed by analyzing 16S rRNA gene amplicons revealed that the dominant bacterial phyla were <i>Proteobacteria</i> and <i>Aquificae</i> (>50% of the microbial composition) in samples collected in 2016 and 2019, respectively. Approximately 10% of the reads obtained in both years were not assigned to any taxonomy. The more detailed phylogenetic positions of the unassigned sequences identified using a clone library and phylogenetic tree showed that they formed a clade that was independent, distantly related to known phyla, and had low similarity (<82%) to all other sequences in available databases. The present results suggest that this novel archaeal phylum-level lineage thrives in boiling hot springs in Japan.</p>

DOI： 10.1264/jsme2.ME21048

CiNii Article

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担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（学術雑誌）

DOI： 10.34565/seibutsukogaku.99.12_621

CiNii Article

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Pseudomonas aeruginosa is a primary bacterial model to study cooperative behaviors because it yields exoproducts such as siderophores and exoproteases that act as public goods and can be exploited by selfish nonproducers behaving as social cheaters. Iron-limited growth medium, mainly casamino acids medium supplemented with transferrin, is typically used to isolate and study nonproducer mutants of the siderophore pyoverdine. However, using a protein as the iron chelator could inadvertently select mutants unable to produce exoproteases, since these enzymes can degrade the transferrin to facilitate iron release. Here we investigated the evolutionary dynamics of pyoverdine and exoprotease production in media in which iron was limited by using either transferrin or a cation chelating resin. We show that concomitant loss of pyoverdine and exoprotease production readily develops in media containing transferrin, whereas only pyoverdine loss emerges in medium treated with the resin. Characterization of exoprotease- and pyoverdine-less mutants revealed loss in motility, different mutations, and large genome deletions (13–33 kb) including Quorum Sensing (lasR, rsal, and lasI) and flagellar genes. Our work shows that using transferrin as an iron chelator imposes simultaneous selective pressure for the loss of pyoverdine and exoprotease production. The unintended effect of transferrin uncovered by our experiments can help to inform the design of similar studies.

DOI： 10.1038/s41396-021-00938-6

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

Because colony formation is essential to seek bacterial functions by the direct observation of phenotype, the diversification of colony formation for culturable bacteria is a big challenge in the research field of Environmental Biotechnology. In this study, the biodiversity of cultivable bacteria (colony or liquid culture) was compared by using Luria-Bertani (LB) medium and waste sewage sludge (WSS) under different dilutions and temperatures. When WSS was used as a bacterial source, whereas the highest number of colonies was found at the concentration of WSS (5%), a particular concentration of LB (10%) or WSS (1%) as a growth medium showed the best number of the operational taxonomic units (OTUs) of colonies. The results of bacterial community structure indicated that there are 1, 8, and 12 bacterial genera found uniquely in the agar plates of LB, 10% LB, and 5% WSS. By contrast, when palm oil mill effluent sludge was used as a bacterial source, the effect of dilution was different with WSS. When comparing the biodiversity between colonies and liquid culture, a high OTU value was observed in the colonies on the plate. In addition, 30°C showed the highest number of colonies in LB, 10% LB, and 5% WSS whereas the best OTUs were observed at 37°C for LB and 10% LB, and at 25°C for 5% WSS. This study demonstrates the diversification of cultivable bacteria through the number of OTUs in diluted LB medium and WSS, which is beneficial to isolate a unique bacterial strain. Key points • Impacts of diluted LB medium and WSS for colony formation were determined. • Difference of concentration of LB and WSS made different effects on colony formation. • Temperature change affected on diluted LB and WSS as media.

DOI： 10.1007/s00253-021-11248-4

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Bone cement based on poly(methyl methacrylate) (PMMA) powder and methyl methacrylate (MMA) liquid is a very popular biomaterial used for the fixation of artificial joints. However, there is a risk of this cement loosening from bone because of a lack of bone-bonding bioactivity. Apatite formation in the body environment is a prerequisite for cement bioactivity. Additionally, suppression of infection during implantation is required for bone cements to be successfully introduced into the human body. In this study, we modified PMMA cement with γ-methacryloxypropyltrimetoxysilane and calcium acetate to introduce bioactive properties and 2-(tert-butylamino)ethyl methacrylate (TBAEMA) to provide antibacterial properties. The long-term antibacterial activity is attributed to the copolymerization of TBAEMA and MMA. As the TBAEMA content increased, the setting time increased and the compressive strength decreased. After soaking in simulated body fluid, an apatite layer was detected within 7 days, irrespective of the TBAEMA content. The cement showed better antibacterial activity against Gram-negative E. Coli than Gram-positive bacteria; however, of the Gram-positive bacteria investigated, B. subtilis was more susceptible than S. aureus.

DOI： 10.1177/08853282211004413

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Alcaligenaceae and Chromatiaceae were previously reported as the specific pollution bioindicators in the receiving river water contaminated by palm oil mill effluent (POME) final discharge. Considering the inevitable sensitivity of bacteria under environmental stresses, it is crucial to assess the survivability of both bacteria in the fluctuated environmental factors, proving their credibility as POME pollution bioindicators in the environment. In this study, the survivability of Alcaligenaceae and Chromatiaceae from facultative pond, algae (aerobic) pond and final discharge were evaluated under varying sets of temperature (25–40°C), pH (pH 7–9) and low/high total suspended solid (TSS) contents of POME collected during low/high crop seasons of oil palm, respectively. Following treatment, the viability status and compositions of the bacterial community were assessed using flow cytometry-based assay and high-throughput Illumina MiSeq, respectively, in correlation with the changes of physicochemical properties. The changes in temperature, pH and TSS indeed changed the physicochemical properties of POME. The functionality of bacterial cells was also shifted where the viable cells and high nucleic acid contents reduced at elevated levels of temperature and pH but increased at high TSS content. Interestingly, the Alcaligenaceae and Chromatiaceae continuously detected in the samples which accounted for more than 0.5% of relative abundance, with a positive correlation with biological oxygen demand (BOD5) concentration. Therefore, either Alcaligenaceae or Chromatiaceae or both could be regarded as the reliable and specific bacterial indicators to indicate the pollution in river water due to POME final discharge despite the fluctuations in temperature, pH and TSS.

DOI： 10.1016/j.jbiosc.2021.04.014

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020, The Author(s). The effect of Aso volcanic limonite was explored in anaerobic digestion using waste sewage sludge (WSS). In this study, methane and hydrogen sulfide were remarkably inhibited when Aso limonite was mixed with WSS as well as a significant reduction of ammonia. Although pH was lowered after adding Aso limonite, methane was still inhibited in neutralized pH condition at 7.0. Hydrolysis stage was not influenced by Aso limonite as supported by the result that a high protease activity was still detected in the presence of the material. However, acidogenesis stage was affected by Aso limonite as indicated by the different productions of organic acids. Acetic acid, was accumulated in the presence of Aso limonite due to the inhibition of methane production, except in the highest concentration of Aso limonite which the production of acetate may be inhibited. Besides, the production of propionate and butyrate reduced in accordance to the increased concentration of Aso limonite. In addition, Archaeal activity (methanogens) in WSS with Aso limonite was low in agreement with the low methane production. Thus, these results indicate that Aso limonite influences the acidogenesis and methanogenesis processes, by which the productions of methane and ammonia were inhibited. On the other hand, in the contactless of Aso limonite during the anaerobic digestion of WSS (Aso limonite was placed in the area of headspace in the vial), Aso limonite had the adsorptive ability for hydrogen sulfide from WSS, but not for methane. This contactless system of Aso limonite may be a practical means to remove hydrogen sulfide without inhibiting methane production as an important bioenergy source.

DOI： 10.1186/s13568-020-01010-w

Kyutacar

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The aquatic ecosystem is continuously threatened by the infiltration and discharge of anthropogenic wastewaters. This issue requires the unending improvement of monitoring systems to become more comprehensive and specific to targeted pollutants. This review intended to elucidate the overall aspects explored by researchers in developing better water pollution monitoring tools in recent years. The discussion is encircled around three main elements that have been extensively used as the basis for the development of monitoring methods, namely the dissolved compounds, bacterial indicator, and nucleic acids. The latest technologies applied in wastewater and surface water mapped from these key players were reviewed and categorized into physicochemical and compound characterizations, biomonitoring, and molecular approaches in taxonomical and functional analyses. Overall, researchers are continuously rallying to enhance the detection of causal source for water pollution through either conventional or mostly advanced approaches focusing on spectrometry, high-throughput sequencing, and flow cytometry technology among others. From this review’s perspective, each pollution evaluation technology has its own advantages and it would be beneficial for several aspects of pollutants assessments to be combined and established as a complementary package for better aquatic environmental management in the long run.

DOI： 10.3390/w12123417

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© MDPI AG. All rights reserved. Polymethyl methacrylate (PMMA)-based bone cement is a popular biomaterial used for fixation of artificial joints. A next-generation bone cement having bone-bonding ability, i.e., bioactivity and antibacterial property is desired. We previously revealed that PMMA cement added with 2-(tert-butylamino)ethyl methacrylate, γ-methacryloxypropyltrimethoxysilane and calcium acetate showed in vitro bioactivity and antibacterial activity. This cement contains calcium acetate at 20% of the powder component. Lower content of the calcium acetate is preferable, because the release of a lot of calcium salt may degrade mechanical properties in the body environment. In the present study, we investigate the effects of calcium acetate content on the setting property and mechanical strength of the cement and apatite formation in simulated body fluid (SBF). The setting time increased and the compressive strength decreased with an increase in calcium acetate content. Although the compressive strength decreased after immersion in SBF for 7 d, all the cements still satisfied the requirements of ISO5833. Apatite was formed in SBF within 7 d on the samples where the calcium acetate content was 5% or more. Therefore, it was found that PMMA cement having antibacterial properties and bioactivity can be obtained even if the amount of the calcium acetate is reduced to 5%.

DOI： 10.3390/ma13214998

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020 Elsevier Ltd This study aims to elucidate the mechanism of carbon partitioning towards starch and triacylglycerol (TAG) under nitrogen stress. Whole-transcriptome analysis was performed using RNA sequencing before being validated with qRT-PCR. The strain, Chlorella vulgaris UPSI-JRM01, exhibited a two-stage response to nitrogen stress: i) carbohydrate accumulation, plastid protein degradation and amino acid biosynthesis; and ii) lipid accumulation, carbohydrate degradation and DNA damage. In the first stage, the genes responsible for plastid protein degradation (CD4A), amino acid biosynthesis (arg, GLSN, AHCY, win1), carbon fixation (RuBisCO, GapC, fba, TK) and starch synthesis (SS, SBE2) were upregulated. In the second stage, the upregulation of genes responsible for starch degradation (SP), glycolysis (PFO, PK, ACS), fatty acid biosynthesis (FabH, accD) and TAG accumulation (DGAT2) were observed. By contrast, the components of photosystem I (PsbD, PsbC, PsbB), photosystem II (PsaA), cytochrome b6/f (PetA, PetG) and F-type ATPase (beta, alpha) were downregulated. The results suggested that nitrogen stress triggered high starch accumulation before the carbon was partitioned towards triacylglycerol (TAG) for long-term energy storage by two different pathways: chloroplastic TAG synthesis and glycerolipid metabolism. The starch build-up functions served as a rapid response to nitrogen stress, whereas subsequent lipid accumulation benefited the long-term storage of energy. This research provides in-depth understanding of the metabolic changes triggered by nitrogen stress.

DOI： 10.1016/j.biombioe.2020.105600

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020 Elsevier Ltd Palm oil mill effluent (POME) contains a substantial amount of nutrients and a significant amount of indigenous microorganisms that consume the nutrients for their growth. In this study, we demonstrated that cultivation of microalgae in POME was able to reduce its carbon and nutrient contents. Three native microalgae isolates, namely, Coelastrella sp. UKM4, Chlamydomonas sp. UKM6 and Scenedesmus sp. UKM9, were cultured in sterilized and unsterilized anaerobic POME to evaluate the microalgae-bacteria interactions for phycoremediation of POME. The results showed higher COD removal in unsterilized POME than in sterilized POME due to microalgae-bacteria consortia interaction. Of the three microalgae isolates, the interaction of Scenedesmus sp. UKM9 with bacteria showed the highest COD and PO43− removal percentages. For NH4+ removal, all three isolates showed removal of more than 80 % in both sterilized and unsterilized POME. Quantitative real-time polymerase chain reaction analysis showed that the quantities of bacteria were constant after the treatment, while the quantities of microalgae changed. Metagenomics analysis showed that the community of bacteria shifted after microalgae cultivation in POME, with Actinobacteria, Bacteroidetes, Planctomycetes and Proteobacteria being the major phyla.

DOI： 10.1016/j.jwpe.2020.101203

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019, Springer-Verlag GmbH Germany, part of Springer Nature. The repurposing of gallium nitrate as an antibacterial, a drug used previously for the treatment of hypercalcemia, is a plausible alternative to combat infections by Pseudomonas aeruginosa, since it has antipseudomonal properties in vitro and in vivo in animal models and in human lung infections. Furthermore, gallium nitrate tolerance in clinical isolates is very rare. Nevertheless, studies on the reference strains PA14 and PAO1 show that resistance against gallium nitrate is achieved by decreasing gallium intracellular levels by increasing the production of pyocyanin. In this work, we induced resistance in a cystic fibrosis P. aeruginosa isolate and explored its resistance mechanisms. This isolated strain, INP-58M, was not a pyocyanin producer, and its pyoverdine levels remained unchanged upon gallium addition. However, it showed higher activities of NADPH-producing enzymes and the antioxidant enzyme SOD when gallium was added, which suggests a better antioxidant response. Remarkably, gallium intracellular levels in the resistant isolate were higher than those of the parental strain at 20 h but lower after 24 h of culture, suggesting that this strain is capable of gallium efflux.

DOI： 10.1007/s00203-019-01777-y

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020 Elsevier Ltd Biochar has proven to be a feasible additive for mitigating nitrogen loss during the composting process. This study aims to evaluate the influence of biochar addition on bacterial community and physicochemical properties changes, including ammonium (NH4+), nitrite (NO2−) and nitrate (NO3−) contents during the composting of poultry manure. The composting was carried out by adding 20% (w/w) of biochar into the mixture of poultry manure and rice straw with a ratio of 2:1, and the same treatment without biochar was prepared as a control. The finished product of control compost recorded the high contents of NO2− and NO3− (366 mg/kg and 600 mg/kg) with reduced the total NH4+ content to 10 mg/kg. Meanwhile, biochar compost recorded a higher amount of total NH4+ content (110 mg/kg) with low NO2− and NO3− (161 mg/kg and 137 mg/kg) content in the final composting material. The principal component analysis showed that the dynamics of dominant genera related to Halomonas, Pusillimonas, and Pseudofulvimonas, all of which were known as nitrifying and denitrifying bacteria, was significantly correlated with the dynamic of NO2− and NO3− content throughout the composting process. The genera related to Pusillimonas, and Pseudofulvimonas appeared as the dominant communities as the NO2− and NO3− increased. In contrast, as the NO2− and NO3− concentration decreased, the Halomonas genus were notably enriched in biochar compost. This study revealed the bacterial community shifts corresponded with the change of physicochemical properties, which provides essential information for a better understanding of monitoring and improving the composting process.

DOI： 10.1016/j.wasman.2020.03.029

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019 Elsevier Ltd Microorganisms are progressively adopted as reliable and specific bioindicators of pollution, with Alcaligenaceae and Chromatiaceae being recently proposed to indicate contamination in river water due to palm oil mill effluent (POME) final discharge. This study was conducted to evaluate the reliability of these bacteria to act as specific bioindicators by assessing their detections in correlation with environmental factors in rivers polluted by POME final discharge when compared to by other pollution sources. By using the Illumina MiSeq high throughput sequencing platform, bacterial diversities and compositions were compared among unpolluted (upstream) river, polluted rivers due to POME final discharge and other rivers polluted by mining, chemical and automotive industries. To correlate between bacterial compositions and metabolisms in rivers subjected to different pollutants sources, the viability and the ratio of the high and low nucleic acids (HNA/LNA) bacterial cells were also compared by using a double staining assay based on flow cytometry. Interestingly, Alcaligenaceae and Chromatiaceae were found only in POME final discharge polluted rivers (>0.15%) but were not detected in rivers polluted by other pollutants. Higher bacterial cell viability was detected in rivers polluted by POME final discharge (86–91%) as compared to the other polluted rivers (15–80%). From the viable fractions of the bacterial cells, higher HNA cells (91–93%) were quantified in the POME final discharge receiving rivers but with only 5–18% in the other rivers. These trends indicated that the nutrients carried by the POME final discharge enhanced the growth of bacteria in the receiving river water that might include Alcaligenaceae and Chromatiaceae. These results suggested that either Alcaligenaceae or Chromatiaceae or both were unique in the POME final discharge polluted rivers but not in the others. They could function as specific and reliable bacterial bioindicators relevant for the establishment of a complementary monitoring system for POME pollutant discharge.

DOI： 10.1016/j.ecolind.2019.106048

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019 Elsevier B.V. Palm oil mill effluent (POME) contains complex and highly biodegradable organic matters so discharging it without appropriate treatment may lead to environmental problems. POME final discharge quality is normally determined based on conventional chemical detection such as by biological oxygen demand (BOD) and chemical oxygen demand (COD). The novelty of the present study is that the toxicity effects of the POME final discharge samples were evaluated based on whole effluent toxicity (WET) and toxicity identification evaluation (TIE) tests using Daphnia magna. The toxicity unit (TU) values were recorded to be in the range from TU = 1.1–11 obtained from WET, and the TIE manipulation tests suggested that a substantial amount of toxic compounds was contained in the POME final discharge. Phenol, 2,6-bis (1,1-dimethylethyl)- and heavy metals such as Cu and Zn were detected in all the effluents and were recognized as being the main toxicants in the POME final discharge. GC/MS analyses also successfully identified cyclic volatile methyl siloxanes; cyclotetrasiloxane, octamethyl- (D4), cyclopentasiloxane, decamethyl- (D5), cyclohexasiloxane, dodecamethyl- (D6). D4 was detected at 0.0148–0.0357 mg/L, which could be potentially toxic. The palm oil industry used only water in the form of steam to process the fruits, and the presence of these compounds might be derived from the detergents and grease used in palm oil mill cleaning and maintenance operations. An appropriate treatment process is thus required to eliminate these toxicants from the POME final discharge. It is recommended that two approaches, chemical-based monitoring as well as biological toxicity-based monitoring, should be utilized for achieving an acceptable quality of POME final discharge in the future.

DOI： 10.1016/j.scitotenv.2019.136277

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記述言語：日本語   掲載種別：研究論文（学術雑誌）

Copyright© 2020, Japanese Society for Food Science and Technology This study investigated the fate of Escherichia coli (E. coli) O157 spiked into 24 commercially available fermented soybean paste (miso) samples. Viable cell numbers of the inoculated E. coli O157 at storage conditions of 5, 10, 20, or 30 ℃ were measured by the agar plate method or most probable number method. Cell numbers of E. coli O157 decreased gradually in all tested samples. The decreases in E. coli O157 numbers depended mainly on the water activity of samples. Decreases in the contamination level of E. coli O157 did not depend on the ingredients of miso samples (malted rice, wheat, and soybean). The obtained results suggest that the commercial risk of accidental E. coli O157 contamination is practically negligible because of high cell mortality during transportation and storage processes at room temperature.

DOI： 10.3136/NSKKK.67.376

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記述言語：英語   掲載種別：研究論文（学術雑誌）

Malaysia is one of the largest producers and exporters of palm oil, thus, a large amount of palm oil mill effluent (POME) is generated through this process. POME contributes to environmental pollution if it is not properly treated. This complex effluent consists of colloidal matters and mainly organic components with more than 90% water. Thus, it is useful to be used as a substrate for fermentative processes, including biohydrogen production. Biohydrogen from POME is a renewable source that can potentially serve as an alternative to substitute fossil fuels. The abundance of POME and the rising price of fossil fuels in the global market create a demand for this source of energy. However, the complexity of the substituents in POME makes the optimisation of this effluent as a substrate in dark fermentation a challenge. This review article explores the important parameters that need to be considered for optimal biohydrogen production, such as the bioreactor operational parameters and the microbial consortium. Besides, the potential of metabolic engineering as a tool to overcome the limitations of the microbial strains to metabolise POME for increased biohydrogen production was also reviewed. However, further research and development are needed to increase the biohydrogen yield on par with commercial demand.

DOI： 10.47836/pjst.28.S2.19

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2020 Hydrogen Energy Publications LLC The four hydrogenase isozymes; hydrogenase 1 (Hyd-1), hydrogenase 2 (Hyd-2), hydrogenase 3 (Hyd-3) and hydrogenase 4 (Hyd-4) of Escherichia coli have been reported for their crucial functions in the hydrogen metabolism; however, their distinctive roles could not be completely understood. In this study, four ideal hydrogenase operon mutants, Δhyb hyc hyf, Δhya hyc hyf, Δhya hyb hyf, and Δhya hyb hyc, in which only a single hydrogenase is intact in the genome, were constructed as well as one quadruple mutant (Δhya hyb hyc hyf) that all four hydrogenase operons were deleted. First, single operon mutants and single-gene mutants for each hydrogenase showed different hydrogen productivity and growth in the anaerobic fermentation, indicating that bacterial phenotype regarding the hydrogen metabolism via the deletion of each operon is different with that of each single gene. Then, 4 triple hydrogenase operon mutants and one quadruple mutant were investigated to evaluate the hydrogen metabolism (hydrogen production and uptake) using glucose or glycerol as a substrate of hydrogen fermentation. With both the carbon sources, only Hyd-2 and Hyd-3 were able to produce hydrogen. Furthermore, all the hydrogenases showed hydrogen uptake activity. In addition, no hydrogen production and hydrogen uptake were detected in the quadruple mutant which does not have all 4 hydrogenases. Hydrogen production from Hyd-2 and Hyd-3 was further confirmed by complementing their operons in the cloning vector pBR322.

DOI： 10.1016/j.ijhydene.2020.10.070

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記述言語：日本語   掲載種別：研究論文（学術雑誌）

<p>味噌に混入させた大腸菌O157の消長を明らかにするために，市販味噌24検体を対象に複数の保存試験区（5，10，20，30 °C）における大腸菌O157生菌数の変動を寒天平板法およびMPN法により観察した．全保存試験区において，味噌混入下の大腸菌O157は増殖することなく段階的に死滅し，その死滅速度と味噌原料（米，麦，大豆）に関連は見られなかった．一方，味噌検体の水分活性値が大腸菌O157の死滅速度に影響を与える可能性を示した．本結果により，大腸菌O157が意図せず味噌に混入した場合であっても，常温での流通，保存の期間に死滅することから，そのリスクは実質的に無視しうるものであると推察された．</p>

DOI： 10.3136/nskkk.67.376

CiNii Article

その他リンク： https://ci.nii.ac.jp/naid/130007934533

記述言語：英語   掲載種別：研究論文（学術雑誌）

© Copyright © 2019 López-Jácome, Garza-Ramos, Hernández-Durán, Franco-Cendejas, Loarca, Romero-Martínez, Nguyen, Maeda, González-Pedrajo, Díaz-Guerrero, Sánchez-Reyes, Díaz-Ramírez and García-Contreras. Pseudomonas aeruginosa is an opportunistic bacterium associated with healthcare infections in intensive care units (ICUs), ventilator-associated pneumonia (VAP), surgical site infections, and burns. This bacterium causes 75% of death in burned patients, since it can develop a persistent biofilm associated with infections, express several virulence factors, and antibiotic-resistance mechanisms. Some of these virulence factors are proteases such as elastase and alkaline protease, or toxic metabolites such as pyocyanin and is one of the few microorganisms able to produce cyanide, which inhibits the cytochrome oxidase of host cells. These virulence factors are controlled by quorum sensing (QS). In this work, 30 P. aeruginosa clinical strains isolated from burned patients from a tertiary hospital in Mexico City were studied. Antibiotic susceptibility tests were done, and virulence factors (elastase, alkaline protease, HCN, and pyocyanin) were determined in presence of an N-acylhomoserine lactonase, AiiM able to hydrolyze a wide range of acyl homoserine lactones. The treatment reduced significantly the activities of elastase and alkaline protease, and the production of pyocyanin and HCN in all producer strains but not the secretion of toxins through the type III secretion system. Our work suggests that AiiM treatment may be an effective therapy to combat P. aeruginosa infection in burn patients.

DOI： 10.3389/fmicb.2019.02657

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Concretionary carbonates in deep-sea methane seep fields are formed as a result of microbial methane degradation, called anaerobic oxidation of methane (AOM). Recently, active microorganisms, including anaerobic methanotrophic archaea, were discovered from methane seep-associated carbonate outcroppings on the seafloor. However sedimentary buried carbonate nodules are a hitherto unknown microbial habitat. In this study, we investigated the microbial community structures in two carbonate nodules collected from a high methane flux site in a gas hydrate field off the Oki islands in the Sea of Japan. The nodules were formed around sulfate-methane interfaces (SMI) corresponding to 0.7 and 2.2 m below the seafloor. Based on a geochemical analysis, light carbon isotopic values ranging from -54.91% to -37.32% were found from the nodules collected at the shallow SMI depth, which were attributed to the high contributions of AOM-induced carbonate precipitation. Signatures of methanotrophic archaeal populations within the sedimentary buried nodule were detected based on microbial community composition analyses and quantitative real-time PCR targeted 16S rRNA, and functional genes for AOM. These results suggest that the buried carbonate nodule currently develops AOM-related microbial communities, and grows depending on the continued AOM under high methane flux conditions.

DOI： 10.3390/geosciences9110463

Kyutacar

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© Copyright © 2019 Loarca, Díaz, Quezada, Guzmán-Ortiz, Rebollar-Ruiz, Presas, Ramírez-Peris, Franco-Cendejas, Maeda, Wood and García-Contreras. Quorum sensing in Pseudomonas aeruginosa controls the production of costly public goods such as exoproteases. This cooperative behavior is susceptible to social cheating by mutants that do not invest in the exoprotease production but assimilate the amino acids and peptides derived by the hydrolysis of proteins in the extracellular media. In sequential cultures with protein as the sole carbon source, these social cheaters are readily selected and often reach equilibrium with the exoprotease producers. Nevertheless, an excess of cheaters causes the collapse of population growth. In this work, using the reference strain PA14 and a clinical isolate from a burn patient, we demonstrate that the initial amount of public goods (exoprotease) that comes with the inoculum in each sequential culture is essential for maintaining population growth and that eliminating the exoprotease in the inoculum leads to rapid population collapse. Therefore, our results suggest that sequential washes should be combined with public good inhibitors to more effectively combat P. aeruginosa infections.

DOI： 10.3389/fmicb.2019.02322

Kyutacar

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019 by the authors. Licensee MDPI, Basel, Switzerland. Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols,with orwithout a ceriumchloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereasMG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.

DOI： 10.3390/polym11101676

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019 Hydrogen Energy Publications LLC Escherichia coli has approximately 4300 open reading frames and about 178 of them are annotated as pseudogenes. The existence of the pseudogenes in E. coli had raised the question of whether they contribute to any cell function. Recently, several pseudogenes have been studied; however, these genes are still considered rare and tend to be ignored as so-called junk. Our recent study has shown several pseudogenes in E. coli are essential in hydrogen metabolism. ydfW is one of the pseudogenes associated with hydrogen metabolism. Here, we found carbon dioxide and nitrate in the fermentation medium of mutant ydfW suggesting that this strain may induce nitrate pathways. The Fdh-N complex is comprised of FdnG, FdnH, and FdnI subunits which correspond to the nitrate-nitrite pathway. This finding is supported by transcription analysis that shows formate dehydrogenase (fdnG) and nitrate reductase (narG) are significantly induced (25- and 18-fold, respectively). FdnG and NarG play a significant role in the reduction of nitrate into nitrite; hence, their genes are induced by the build-up of nitrate. Based on in silico docking analysis, we predict that YdfW is covalently bound to the Fdh-N complex in the cytoplasm. Hence, deletion of ydfW is expected has influenced the nitrate-nitrite pathway. Subsequently, the situation will affect the hydrogen production in E. coli.

DOI： 10.1016/j.ijhydene.2019.04.228

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Quorum sensing (QS) plays a key role in activating bacterial functions through small molecules called autoinducers. In this study, the QS of Gram-negative bacteria in waste sewage sludge (WSS) was downregulated by adding the quorum quenching enzyme, AiiM lactonase, which cleaved the acyl-homoserine lactone (AHL) autoinducer signals from Gram-negative bacteria, and subsequently methane production was inhibited by over 400%. The pH was lowered after 2 days in the anaerobic fermentation whereas protease activity at the hydrolysis step was almost the same with or without AiiM. The production of acetic acid significantly increased during the fermentation in the presence of AiiM. The bacterial community at day 2 indicated that the population of Gram-positive bacteria increased in the presence of AiiM, and the percentage of Gram-negative bacteria decreased in the WSS containing AiiM. The change in the bacterial community in the presence of AiiM may be due to the different antimicrobial agents produced in the WSS because some of the Gram-positive bacteria were killed by adding the solid-phase extraction (SPE) fraction from the WSS without AiiM. In contrast, the SPE fraction with AiiM had reduced bactericidal activity against Gram-negative bacteria. Thus, bacterial signaling between Gram-negative bacteria is critical for methane production by the microbial consortia.

DOI： 10.1007/s00253-018-9553-9

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2019, Japanese Society of Microbial Ecology. All rights reserved. Despite efforts to address the composition of the microbial community during the anaerobic treatment of palm oil mill effluent (POME), its composition in relation to biodegradation in the full-scale treatment system has not yet been extensively examined. Therefore, a thorough analysis of bacterial and archaeal communities was performed in the present study using MiSeq sequencing at the different stages of the POME treatment, which comprised anaerobic as well as facultative anaerobic and aerobic processes, including the mixed raw effluent (MRE), mixing pond, holding tank, and final discharge phases. Based on the results obtained, the following biodegradation processes were suggested to occur at the different treatment stages: (1) Lactobacillaceae (35.9%) dominated the first stage, which contributed to high lactic acid production; (2) the higher population of Clostridiaceae in the mixing pond (47.7%) and Prevotellaceae in the holding tank (49.7%) promoted acetic acid production; (3) the aceticlastic methanogen Methanosaetaceae (0.6–0.8%) played a role in acetic acid degradation in the open digester and closed reactor for methane generation; (4) Syntrophomonas (21.5–29.2%) appeared to be involved in the degradation of fatty acids and acetic acid by syntrophic cooperation with the hydrogenotrophic methanogen, Methanobacteriaceae (0.6–1.3%); and (5) the phenols and alcohols detected in the early phases, but not in the final discharge phase, indicated the successful degradation of lignocellulosic materials. The present results contribute to a better understanding of the biodegradation mechanisms involved in the different stages of the full-scale treatment of POME.

DOI： 10.1264/jsme2.ME18104

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CiNii Article

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018 Elsevier Ltd This study was undertaken to confirm the reliability of the proposed potential bioindicators, Alcaligenaceae and Chromatiaceae to specifically indicate contamination in river water by palm oil mill effluent (POME) final discharge. The use of bioindicators could assist in determining the specific cause of contamination in water bodies. Alcaligenaceae and Chromatiaceae were shown by a previous study to be present in river water contaminated with the final discharge from the treatment of POME. In the present study, the compositions of the bacterial communities in the POME final discharge obtained from four different palm oil mills which used different biotreatments of POME were elucidated using high-throughput MiSeq. The four POME final discharges studied showed different species richness and evenness among them. However, the bacterial community compositions in the different final discharges exhibited almost similar patterns in that the phylum Proteobacteria was dominant in all the samples. The proposed bioindicators, the Alcaligenaceae and Chromatiaceae families, were found to be present in all the four final discharges despite the different characteristics of the mills and the different biotreatment processes used by them. These bioindicators were also strongly and positively correlated with the biochemical oxygen demand (BOD5) concentration. This makes them reliable bacterial indicators to detect the presence of POME final discharge in river water.

DOI： 10.1016/j.ecolind.2018.08.007

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg−1protein to 6 µmol mg−1protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.

DOI： 10.1007/s13205-018-1461-2

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018 The Author(s). Background: We previously reversed methanogenesis in microbial fuel cells (MFCs) to produce electricity for the first time from methane by combining an engineered archaeal strain that produces methyl-coenzyme M reductase from unculturable anaerobic methanotrophs (to capture methane and secrete acetate) with Geobacter sulfurreducens (to produce electrons from the generated acetate) and methane-acclimated sludge (to provide electron shuttles). Results: Here, the power density in MFCs was increased 77-fold to 5216 mW/m2and the current density in MFCs was increased 73-fold to 7.3 A/m2by reducing the surface area of the cathode (to make reasonable comparisons to other MFCs), by changing the order the strains of the consortium were added to the anode compartment, and by adding additional electron carriers (e.g., humic acids and cytochrome C). Conclusions: This power density and current density are comparable to the best for any MFC, including those with Shewanella and Geobacter spp. that utilize non-gaseous substrates. In addition, we demonstrate the methane MFC may be used to power a fan by storing the energy in a capacitor. Hence, MFCs that convert methane to electricity are limited by electron carriers.

DOI： 10.1186/s13068-018-1208-7

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018 American Dairy Science Association The activation of phagocytosis is one important approach to clearing pathogenic cells in a host. This study evaluated the ability of probiotic lactobacilli to induce phagocytic activity as well as the clearance of a periodontal pathogen, Aggregatibacter actinomycetemcomitans. First, the activation of phagocytosis was found by using lyophilized dead cells. Probiotic Lactobacillus strains significantly enhanced the phagocytic activity of macrophage cells, indicating that the probiotic lactobacilli have a remarkable ability to stimulate the macrophages. Essentially, 3 Lactobacillus strains tested did not have any critical toxic effect on the murine macrophage, and Lactobacillus johnsonii NBRC 13952 showed the least cytotoxic effect on the RAW264.7 macrophages. The expression of classically activated macrophage markers, IL-1β, and cluster of differentiation 80 increased by L. johnsonii NBRC 13952; however, there was no significant difference for IL-18. The highest phagocytic activity by macrophages was found in a condition in which the macrophage activated by L. johnsonii NBRC 13952 functions to kill the cells of A. actinomycetemcomitans. Correlating with the result, a high amount of hypodiploid DNA (SubG1) was detected from the macrophage cells stimulated by L. johnsonii NBRC 13952. Taken together, the results suggest that macrophages activated by the Lactobacillus strain can facilitate the phagocytosis of A. actinomycetemcomitans cells by linking with enhanced apoptotic activities. In conclusion, L. johnsonii NBRC 13952 has a certain role in activating the RAW264.7 macrophages, thereby counteracting the infection of A. actinomycetemcomitans.

DOI： 10.3168/jds.2017-14355

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018 Castañeda-Tamez, Ramírez-Peris, Pérez-Velázquez, Kuttler, Jalalimanesh, Saucedo-Mora, Jiménez-Cortés, Maeda, González, Tomás, Wood and García-Contreras. Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.

DOI： 10.3389/fmicb.2018.01348

Scopus

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Efficient approaches for the utilization of waste sewage sludge have been widely studied. One of them is to use it for the bioenergy production, specifically methane gas which is well-known to be driven by complex bacterial interactions during the anaerobic digestion process. Therefore, it is important to understand not only microorganisms for producing methane but also those for controlling or regulating the process. In this study, azithromycin analogs belonging to macrolide, ketolide, and lincosamide groups were applied to investigate the mechanisms and dynamics of bacterial community in waste sewage sludge for methane production. The stages of anaerobic digestion process were evaluated by measuring the production of intermediate substrates, such as protease activity, organic acids, the quantification of bacteria and archaea, and its community dynamics. All azithromycin analogs used in this study achieved a high methane production compared to the control sample without any antibiotic due to the efficient hydrolysis process and the presence of important fermentative bacteria and archaea responsible in the methanogenesis stage. The key microorganisms contributing to the methane production may be Clostridia, Cladilinea, Planctomycetes, and Alphaproteobacteria as an accelerator whereas Nitrosomonadaceae and Nitrospiraceae may be suppressors for methane production. In conclusion, the utilization of antibiotic analogs of macrolide, ketolide, and lincosamide groups has a promising ability in finding the essential microorganisms and improving the methane production using waste sewage sludge.

DOI： 10.1007/s00253-018-9003-8

Scopus

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担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018, Springer-Verlag GmbH Germany, part of Springer Nature. Escherichia coli has been a robust host strain for much biological research, in particular, research in metabolic engineering, protein engineering, and heterologous gene expression. In this mini review, to understand bacterial hydrogen production by E. coli, the effect of glucose and glycerol metabolism on hydrogen production is compared, and the current approaches to enhance hydrogen production from glycerol as a substrate are reviewed. In addition, the argument from past to present on the functions of E. coli hydrogenases, hydrogenase 1, hydrogenase 2, hydrogenase 3, and hydrogenase 4 is summarized. Furthermore, based on the literature that the E. coli formate-hydrogen lyase is essential for bacterial hydrogen production via recombinant hydrogenases, research achievements from the past regarding heterologous production of hydrogenase are rethought.

DOI： 10.1007/s00253-018-8752-8

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2017 Elsevier Ltd The microbiological effects of palm oil mill effluent (POME) final discharge upon a receiving river were assessed in this study by using the nucleic acid double staining assay based on flow cytometry. The functional status of the bacterial community at the single-cell level was determined with regards to their abundance, viability and nucleic acid content to monitor the effects of POME final discharge on the affected river. The effluent resulted in the increment of the total cell concentration (TCC) and viable cells which were correlated with the increment of biological oxygen demand (BOD5) and total organic carbon (TOC) concentrations in the receiving river. The shift of low nucleic acid (LNA) to high nucleic acid (HNA) bacterial cells in the affected river suggested the transformation of dormant to active cells due to the POME final discharge. This is the first study to report on the shift of LNA/HNA ratios which may serves as a potential bioindicator in the screening of the anthropogenic effects due to POME final discharge in river water with originally high LNA proportions. Monitoring the effluent discharge at low trophic level using flow cytometry is a rapid and sensitive approach when compared to the current physicochemical assessment method. This approach allows for the screening of river water contamination caused by POME final discharge prior to a full assessment using the recently proposed specific bacterial indicators.

DOI： 10.1016/j.ecolind.2017.10.020

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2017 Elsevier B.V. To achieve industrial (R,R)-2,3-butanediol ((R,R)-2,3-BDL) production from various lignocellulosic biomass, it is essential to develop an efficient fermentation process to overcome the challenge of carbon catabolite repression (CCR). The present study comprehensively investigated the effects of different glucose/xylose mixtures and fermentation strategies on (R,R)-2,3-BDL production using a nonpathogenic strain of Paenibacillus polymyxa. In both batch and fed-batch mode, we observed slow sugar utilization and a lower productivity. By employing cell-recycling continuous fermentation system for (R,R)-2,3-BDL production from mixed sugar, the volumetric productivity was significantly increased, by an average of 2-fold higher compared to alternative fermentation modes. Moreover, the (R,R)-2,3-BDL titer, volumetric productivity and yield achieved 18.80 g/l, 1.13 g/l/h and 0.313 g/g when corn stover hydrolysates (CSH)was used as substrate. The results suggested that the reported fermentation system could efficiently eliminate CCR to improve (R,R)-2,3-BDL productivity. Thus, it had great potential for the industrial-scale (R,R)-2,3-BDL production from lignocellulosic hydrolysates.

DOI： 10.1016/j.cej.2017.09.097

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記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2018, University of Malaya. All rights reserved. Polyhydroxyalkanoate (PHA) is a linear polyester produced through the fermentation of sugar or lipid. Biosynthesis of PHA comprises three enzymes known as acetyl-CoA acetyltransferase (phaA), acetoacetyl-CoA reductase (phaB) and PHA synthase (phaC). Comamonas sp. is one of the strains commonly used for PHA production. In order to develop higher PHA production from bacterial respond strategy, PHA biosynthesis operon of Comamonas sp. EB172 was introduced into Escherichia coli BW25113 through a pGEM-T vector. E. coli was chosen due to the complete genome information available and the absence of depolymerisation gene, phaZ. In this study, the deletion of several single genes, which are frdC, gltA, and pta, was found to be associated with PHA metabolism activity in E. coli BW25113. P1 transduction was performed to construct multiple genes knockout. The engineered strain, E. coli BW25113 frdCgltApta::kan/pGEM’-phaCAB Co , yielded the highest PHA production at 64 wt.% with 1.4 fold higher than that of control strain of E. coli BW25113/pGEM’-phaCAB Co . This strain is potential for industrial application for higher PHA production from E. coli.

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.3389/fmicb.2017.01669

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.jclepro.2017.07.129

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ecolind.2017.07.038

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1039/c7ra04049k

Kyutacar

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.apenergy.2017.05.171

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijhydene.2016.11.188

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.2174/1568026617666170105144104

担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1186/s41021-016-0047-7

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s00253-016-7767-2.

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1002/jbm.a.35827.

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1371/journal.pone.0159466.

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.3934/microbiol.2016.2.138.

記述言語：英語   掲載種別：研究論文（学術雑誌）

© 2016, Penerbit Universiti Kebangsaan Malaysia. All rights reserved.Large numbers of glycerol-consuming bacteria are present in nature; hence bioconversion of glycerol into biofuel which is bioethanol is one of the interests. The effective screening procedure is needed to screen and isolate broad ranges of bacteria from environment. The screening method was modified based on enzymatic oxidation of ethanol, which is correlated to reduction of 2,6-dichlorophenol-indophenol dye that resulted in the formation of yellow zone. Approximately 300 colonies were able to grow on minimal media using glycerol as sole carbon. Only about 70 isolates showed positive result when using the modified ethanol production assay after pre-screening stage. The formation of decolourized zone was apparent using modified assay containing 5 mL/L of 0.05M 2,6-dichlorophenol-indophenol, 10 mL of reaction mixture and 500 μl/L of enzyme, respectively. The ethanol production capability of the isolates was further proven by anaerobic fermentation as a quantitative method. This modified method is applicable in screening for ethanol producer from glycerol as carbon source allows rapid and more bacteria can be screened.

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

This study implemented the pyrosequencing technique and real-time quantitative PCR to determine the prevalence of foodborne pathogenic bacteria (FPB) and as well as the ecological correlations of background biota and FPB present on restaurant cutting boards (CBs) collected in Seri Kembangan, Malaysia. The prevalence of FPB in high background biota (HBB) was lower (0.24%) compared to that of low background biota (LBB) (0.54%). In addition, a multiple linear regression analysis indicated that only HBB had a significant ecological correlation with FPB. Furthermore, statistical analysis revealed that the combinations of Clostridiales, Flavobacteriales, and Lactobacillales orders in HBB had significant negative associations with FPB, suggesting that these bacteria may interact to ensure survivability and impair the growth of pathogenic bacteria.

DOI： 10.3934/MICROBIOL.2016.2.138

Scopus

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記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1038/ismej.2015.84

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1093/femspd/ftv040

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.12998/wjcc.v3.i7.575.

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijfoodmicro.2015.01.022

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.apenergy.2014.12.069

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s00253-014-6338-7

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1038/ismej.2014.98

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.chemosphere.2014.08.055

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.biortech.2014.10.005

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s10295-014-1446-z

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s00253-014-5600-3

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1111/jam.12410

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Malaysia   Kuala Lumpur   2014年02月09日  -  2014年02月11日

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1111/2049-632X.12105

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.biortech.2013.10.022

Scopus

担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijmm.2013.07.009

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Jeju   2013年11月12日  -  2013年11月14日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Jeju   2013年11月12日  -  2013年11月14日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Jeju   2013年11月12日  -  2013年11月14日

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1128/AEM.02378-13

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.bbrc.2013.09.016

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.biortech.2013.03.003

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijhydene.2013.06.065

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Japan   Narita   2013年07月05日  -  2013年07月08日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Japan   Narita   2013年07月05日  -  2013年07月08日

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1007/s12033-013-9647-7

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記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.chemosphere.2013.01.114

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijhydene.2013.01.031

Scopus

担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

日本   沖縄   2012年12月14日  -  2012年12月16日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijhydene.2012.08.115

Scopus

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

日本   沖縄   2012年12月14日  -  2012年12月16日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Deagu   2012年09月16日  -  2012年09月21日

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1590/S1517-83822012000200011

Scopus

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1038/ismej.2011.122

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1111/j.1751-7915.2011.00282.x

Scopus

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Gyung-ju   2011年12月02日  -  2011年12月04日

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Gyung-ju   2011年12月02日  -  2011年12月04日

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Korea   Gyung-ju   2011年12月02日  -  2011年12月04日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.biortech.2011.08.098

Scopus

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Spain   Malaga   2011年09月14日  -  2011年09月16日

担当区分：責任著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Spain   Malaga   2011年09月14日  -  2011年09月16日

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Japan   Sapporo   2011年09月09日  -  2011年09月10日

担当区分：責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Kitakyushu   2010年11月20日  -  2010年11月21日

記述言語：英語   掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.bbrc.2010.09.026

Scopus

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Italy   Rimini   2010年09月14日  -  2010年09月18日

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Italy   Rimini   2010年09月14日  -  2010年09月18日

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

France   Paris   2010年03月22日  -  2010年03月24日

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

France   Paris   2010年03月22日  -  2010年03月24日

担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：英語   掲載種別：研究論文（学術雑誌）

Kobe   2009年11月24日  -  2009年11月28日

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（学術雑誌）

主要雑誌

記述言語：英語   掲載種別：研究論文（学術雑誌）

主要雑誌

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）

記述言語：日本語   掲載種別：研究論文（大学，研究機関等紀要）

記述言語：英語   掲載種別：研究論文（学術雑誌）

主要雑誌

担当区分：責任著者   記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：日本語   掲載種別：記事・総説・解説・論説等（その他）

記述言語：英語   掲載種別：研究論文（学術雑誌）

主要雑誌

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

China   Dalian   2008年10月12日  -  2008年10月17日

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（学術雑誌）

主要雑誌

記述言語：日本語   掲載種別：研究論文（学術雑誌）

主要雑誌

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

America   Boston   2008年06月  -  2008年06月

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

America   Boston   2008年06月  -  2008年06月

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

代表的研究業績

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

代表的研究業績

記述言語：英語   掲載種別：研究論文（学術雑誌）

代表的研究業績

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

America   Austin   2007年10月  -  2007年10月

主要論文集（会議）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

代表的研究業績

記述言語：英語   掲載種別：研究論文（学術雑誌）

代表的研究業績

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）

Germany   Leipzig   2006年07月09日  -  2006年07月14日

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：英語   掲載種別：研究論文（学術雑誌）

記述言語：日本語   掲載種別：研究論文（学術雑誌）

記述言語：日本語   掲載種別：研究論文（学術雑誌）

開催期間： 2019年11月12日 - 2019年11月14日   記述言語：英語  

開催期間： 2019年11月12日 - 2019年11月14日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月11日 - 2019年11月12日   記述言語：英語  

開催期間： 2019年11月08日 - 2019年11月09日   記述言語：日本語  

開催期間： 2019年11月08日 - 2019年11月09日   記述言語：日本語  

開催期間： 2019年11月08日 - 2019年11月09日   記述言語：日本語  

開催期間： 2019年09月16日 - 2019年09月18日   記述言語：日本語  

開催期間： 2019年07月13日   記述言語：日本語  

開催期間： 2019年07月13日   記述言語：日本語  

開催期間： 2019年07月13日   記述言語：日本語  

開催期間： 2019年06月15日 - 2019年06月16日   記述言語：日本語  

開催期間： 2019年06月15日 - 2019年06月16日   記述言語：日本語  

開催期間： 2019年05月11日 - 2019年05月13日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年12月15日 - 2018年12月16日   記述言語：英語  

開催期間： 2018年11月12日   記述言語：英語  

開催期間： 2018年11月12日   記述言語：英語  

開催期間： 2018年11月12日   記述言語：英語  

開催期間： 2018年11月12日   記述言語：英語  

開催期間： 2018年11月12日   記述言語：英語  

開催期間： 2018年09月05日 - 2018年09月07日   記述言語：日本語   開催地：関西大学  

開催期間： 2018年06月30日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2018年06月30日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2018年06月30日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2018年06月25日 - 2018年06月26日   記述言語：日本語   開催地：筑波大学  

開催期間： 2018年06月25日 - 2018年06月26日   記述言語：日本語   開催地：筑波大学  

開催期間： 2018年06月25日 - 2018年06月26日   記述言語：日本語   開催地：筑波大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：英語   開催地：北海道大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：日本語   開催地：北海道大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：日本語   開催地：北海道大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：日本語   開催地：北海道大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：英語   開催地：北海道大学  

開催期間： 2018年03月14日 - 2018年03月16日   記述言語：英語   開催地：北海道大学  

開催期間： 2017年10月12日   記述言語：日本語   開催地：AIM3階　314・315会議室  

開催期間： 2017年09月11日 - 2017年09月14日   記述言語：日本語   開催地：早稲田大学西早稲田キャンパス  

開催期間： 2017年08月29日 - 2017年08月31日   記述言語：日本語   開催地：東北大学川内北キャンパス  

開催期間： 2017年08月29日 - 2017年08月31日   記述言語：日本語   開催地：東北大学川内北キャンパス  

開催期間： 2017年03月15日 - 2017年03月17日   記述言語：日本語   開催地：熊本大学黒髪キャンパス  

開催期間： 2017年03月15日 - 2017年03月17日   記述言語：日本語   開催地：熊本大学黒髪キャンパス  

開催期間： 2016年09月28日 - 2016年09月30日   記述言語：日本語  

開催期間： 2016年06月13日 - 2016年06月14日   記述言語：日本語  

開催期間： 2016年06月13日 - 2016年06月14日   記述言語：日本語  

開催期間： 2016年03月27日 - 2016年03月29日   記述言語：日本語  

開催期間： 2016年03月27日 - 2016年03月29日   記述言語：日本語  

開催期間： 2016年03月16日 - 2016年03月18日   記述言語：英語   開催地：アスティとくしま  

開催期間： 2016年03月16日 - 2016年03月18日   記述言語：日本語   開催地：アスティとくしま  

開催期間： 2016年03月16日 - 2016年03月18日   記述言語：日本語   開催地：アスティとくしま  

開催期間： 2016年03月16日 - 2016年03月18日   記述言語：日本語   開催地：アスティとくしま  

開催期間： 2016年03月16日 - 2016年03月18日   記述言語：日本語   開催地：アスティとくしま  

開催期間： 2016年02月27日   記述言語：日本語   開催地：佐賀大学本庄キャンパス  

開催期間： 2016年02月27日   記述言語：日本語   開催地：佐賀大学本庄キャンパス  

開催期間： 2016年02月27日   記述言語：日本語   開催地：佐賀大学本庄キャンパス  

開催期間： 2016年02月27日   記述言語：日本語   開催地：佐賀大学本庄キャンパス  

開催期間： 2015年10月26日 - 2015年10月28日   記述言語：日本語   開催地：城山観光ホテル  

開催期間： 2015年10月26日 - 2015年10月28日   記述言語：英語   開催地：城山観光ホテル  

開催期間： 2015年10月26日 - 2015年10月28日   記述言語：英語   開催地：城山観光ホテル  

開催期間： 2015年09月12日 - 2015年09月13日   記述言語：英語   開催地：アクトシティ浜松  

開催期間： 2015年06月29日 - 2015年06月30日   記述言語：日本語  

開催期間： 2015年06月29日 - 2015年06月30日   記述言語：日本語  

開催期間： 2015年06月11日 - 2015年06月12日   記述言語：英語   開催地：北九州国際会議場  

開催期間： 2015年06月11日 - 2015年06月12日   記述言語：英語   開催地：北九州国際会議場  

開催期間： 2015年03月26日 - 2015年03月28日   記述言語：日本語  

開催期間： 2015年03月16日 - 2015年03月18日   記述言語：日本語  

開催期間： 2015年03月16日 - 2015年03月18日   記述言語：日本語  

開催期間： 2015年03月16日 - 2015年03月18日   記述言語：日本語  

開催期間： 2015年02月28日   記述言語：日本語  

開催期間： 2015年02月28日   記述言語：日本語  

開催期間： 2015年02月28日   記述言語：日本語  

開催期間： 2014年12月06日   記述言語：日本語  

開催期間： 2014年12月06日   記述言語：日本語  

開催期間： 2014年12月06日   記述言語：日本語  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：英語   開催地：アクトシティ浜松  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：英語   開催地：アクトシティ浜松  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：英語   開催地：アクトシティ浜松  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：日本語   開催地：アクトシティ浜松  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：日本語   開催地：アクトシティ浜松  

開催期間： 2014年10月21日 - 2014年10月24日   記述言語：英語   開催地：アクトシティ浜松  

開催期間： 2014年03月17日 - 2014年03月19日   記述言語：日本語   開催地：東北大学川内北キャンパス  

開催期間： 2014年03月17日 - 2014年03月19日   記述言語：日本語   開催地：東北大学川内北キャンパス  

開催期間： 2014年03月17日 - 2014年03月19日   記述言語：日本語   開催地：東北大学川内北キャンパス  

開催期間： 2014年03月17日 - 2014年03月19日   記述言語：英語   開催地：東北大学川内北キャンパス  

開催期間： 2014年03月01日   記述言語：英語   開催地：独立行政法人国立高等専門学校機構鹿児島工業高等専門学校  

開催期間： 2014年03月01日   記述言語：日本語   開催地：独立行政法人国立高等専門学校機構鹿児島工業高等専門学校  

開催期間： 2013年11月07日 - 2013年11月09日   記述言語：英語  

開催期間： 2013年11月07日 - 2013年11月09日   記述言語：英語  

開催期間： 2013年11月07日 - 2013年11月09日   記述言語：英語  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月30日 - 2013年10月01日   記述言語：英語   開催地：UPM Serdang, Malaysia  

開催期間： 2013年09月18日 - 2013年09月20日   記述言語：日本語   開催地：広島国際会議場  

開催期間： 2013年09月18日 - 2013年09月20日   記述言語：日本語   開催地：広島国際会議場  

開催期間： 2013年09月18日 - 2013年09月20日   記述言語：英語   開催地：広島国際会議場  

開催期間： 2013年09月18日 - 2013年09月20日   記述言語：英語   開催地：広島国際会議場  

開催期間： 2013年09月18日 - 2013年09月20日   記述言語：日本語   開催地：広島国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：英語   開催地：北九州国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：英語   開催地：北九州国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：日本語   開催地：北九州国際会議場  

開催期間： 2013年05月30日 - 2013年06月01日   記述言語：英語   開催地：北九州国際会議場  

開催期間： 2013年03月18日 - 2013年03月20日   記述言語：日本語   開催地：幕張メッセ  

開催期間： 2013年03月11日 - 2013年03月13日   記述言語：日本語  

開催期間： 2013年03月11日 - 2013年03月13日   記述言語：日本語  

開催期間： 2013年02月16日   記述言語：日本語  

開催期間： 2013年02月16日   記述言語：英語  

開催期間： 2013年02月16日   記述言語：日本語  

開催期間： 2013年01月29日 - 2013年01月30日   記述言語：英語  

開催期間： 2012年10月23日 - 2012年10月26日   記述言語：英語  

開催期間： 2012年06月25日 - 2012年06月26日   記述言語：英語  

開催期間： 2012年06月25日 - 2012年06月26日   記述言語：日本語  

開催期間： 2012年06月25日 - 2012年06月26日   記述言語：英語  

開催期間： 2012年03月14日 - 2012年03月16日   記述言語：日本語   開催地：日本 東京都　東洋大学  

開催期間： 2012年03月14日 - 2012年03月16日   記述言語：日本語   開催地：日本 東京都　東洋大学  

開催期間： 2012年03月14日 - 2012年03月16日   記述言語：日本語   開催地：日本 東京都　東洋大学  

開催期間： 2011年10月28日   記述言語：英語   開催地：Kitayushu, Japan  

開催期間： 2011年10月28日   記述言語：英語   開催地：Kitayushu, Japan  

開催期間： 2011年06月20日 - 2011年06月21日   記述言語：日本語   開催地：日本 東京都　東京大学  

開催期間： 2011年06月20日 - 2011年06月21日   記述言語：日本語   開催地：日本 東京都　東京大学  

開催期間： 2011年06月20日 - 2011年06月21日   記述言語：日本語   開催地：日本 東京都　東京大学  

開催期間： 2010年11月07日 - 2010年11月12日   記述言語：英語   開催地： USA Salt Lake City  

開催期間： 2010年06月21日 - 2010年06月22日   記述言語：日本語   開催地：日本 仙台市  

開催期間： 2010年06月20日 - 2010年06月21日   記述言語：日本語   開催地：日本 仙台市  

開催期間： 2010年03月15日 - 2010年03月17日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2010年03月15日 - 2010年03月17日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2010年03月15日 - 2010年03月17日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2009年12月09日 - 2009年12月12日   記述言語：英語   開催地：日本 横浜  

開催期間： 2009年11月13日   記述言語：英語  

開催期間： 2009年10月15日   記述言語：英語   開催地：日本 北九州市  

開催期間： 2009年06月23日 - 2009年06月24日   記述言語：日本語   開催地：日本 東京  

開催期間： 2009年06月23日 - 2009年06月24日   記述言語：日本語   開催地：日本 東京  

開催期間： 2009年03月16日 - 2009年03月18日   記述言語：日本語   開催地：日本 山口市  

開催期間： 2009年01月16日   記述言語：日本語   開催地：日本 北九州市  

開催期間： 2009年01月16日   記述言語：日本語   開催地：日本 北九州市  

開催期間： 2008年12月09日 - 2008年12月12日   記述言語：英語   開催地：日本 神戸市  

開催期間： 2008年10月09日 - 2008年10月10日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2008年10月09日 - 2008年10月10日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2008年10月09日 - 2008年10月10日   記述言語：日本語   開催地：日本 福岡市  

開催期間： 2008年09月03日 - 2008年09月05日   記述言語：日本語   開催地：日本 名古屋市  

開催期間： 2008年08月27日 - 2008年08月29日   記述言語：日本語   開催地：日本 仙台市  

開催期間： 2008年06月25日 - 2008年06月27日   記述言語：日本語   開催地：日本 つくば  

開催期間： 2008年06月25日 - 2008年06月27日   記述言語：日本語   開催地：日本 つくば  

開催期間： 2008年06月25日 - 2008年06月27日   記述言語：日本語   開催地：日本 つくば  

開催期間： 2008年05月23日   記述言語：英語   開催地：日本 北九州市  

開催期間： 2008年03月19日 - 2008年03月21日   記述言語：日本語   開催地：日本 名古屋  

開催期間： 2007年10月11日 - 2007年10月12日   記述言語：日本語   開催地：日本 北九州市  

開催期間： 2007年10月11日 - 2007年10月12日   記述言語：日本語   開催地：日本 北九州市  

開催期間： 2007年10月11日 - 2007年10月12日   記述言語：日本語   開催地：日本 北九州市  

開催期間： 2007年06月26日 - 2007年06月27日   記述言語：日本語   開催地： 吹田市  

開催期間： 2007年06月26日 - 2007年06月27日   記述言語：日本語   開催地：日本 吹田市  

開催期間： 2007年06月20日 - 2007年06月22日   記述言語：日本語   開催地：日本 北九州市  

講演種別：招待講演   開催地：UNAM  

講演種別：招待講演   開催地：BEXCO  

講演種別：招待講演  

開催期間： 2011年10月28日   発表言語：英語   講演種別：特別講演   開催地：Kitayushu, Japan  

受賞国：日本国

受賞国：日本国

受賞国：日本国

受賞国：日本国

受賞国：日本国

受賞国：日本国

受賞国：日本国

受賞国：日本国

頭脳循環を活性化する若手研究者海外派遣プログラム
課題番号：R2217

平成21年度優秀若手研究者海外派遣事業
課題番号：21-7007