花田 耕介 (ハナダ コウスケ)

HANADA Kosuke

写真a

職名

教授

研究室住所

福岡県飯塚市川津680-4

研究分野・キーワード

ゲノム解析

取得学位 【 表示 / 非表示

  • 総合研究大学院大学 -  博士(理学)  2003年03月

学内職務経歴 【 表示 / 非表示

  • 2020年06月
    -
    継続中

    九州工業大学   大学院情報工学研究院   生命化学情報工学研究系   教授  

  • 2019年04月
    -
    2020年05月

    九州工業大学   大学院情報工学研究院   生命化学情報工学研究系   准教授  

  • 2017年04月
    -
    2019年03月

    九州工業大学   大学院情報工学研究院   生命情報工学研究系   准教授  

  • 2012年10月
    -
    2017年03月

    九州工業大学   若手研究者フロンティア研究アカデミー   准教授  

 

論文 【 表示 / 非表示

  • Contribution of Functional Divergence Through Copy Number Variations to the Inter-Species and Intra-Species Diversity in Specialized Metabolites

    Shirai K., Hanada K.

    Frontiers in Plant Science    10   2019年11月  [査読有り]  [招待有り]

     概要を見る

    © Copyright © 2019 Shirai and Hanada. There is considerable diversity in the specialized metabolites within a single plant species (intra-species) and among different plant species (inter-species). The functional divergence associated with gene duplications largely contributes to the inter-species diversity in the specialized metabolites, whereas the intra-species diversity is due to gene dosage changes via gene duplications [i.e., copy number variants (CNVs)] at the intra-species level of evolution. This is because CNVs are thought to undergo associated with less functional divergence at the intra-species level of evolution. However, functional divergence caused by CNVs may induce specialized metabolite diversity at the intra-species and inter-species levels of evolution. We herein discuss the functional divergence of CNVs in metabolic quantitative trait genes (mQTGs). We focused on 5,654 previously identified mQTGs in 270 Arabidopsis thaliana accessions. The ratio of nonsynonymous to synonymous variations tends to be higher for mQTGs with CNVs than for mQTGs without CNVs within A. thaliana accessions, suggesting that CNVs are responsible for the functional divergence among mQTGs at the intra-species level of evolution. To evaluate the contribution of CNVs to inter-species diversity, we calculated the ratio of nonsynonymous to synonymous substitutions in the Arabidopsis lineage. The ratio tends to be higher for the mQTGs with CNVs than for the mQTGs without CNVs. Additionally, we determined that mQTGs with CNVs are subject to positive selection in the Arabidopsis lineage. Our data suggest that CNVs are closely related to functional divergence contributing to adaptations via the production of diverse specialized metabolites at the intra-species and inter-species levels of evolution.

    DOI Scopus

  • Tyr82 amino acid mutation in pb1 polymerase induces an influenza virus mutator phenotype

    Naito T., Shirai K., Mori K., Muratsu H., Ushirogawa H., Ohniwa R., Hanada K., Saito M.

    Journal of Virology    93 ( 22 )   2019年11月  [査読有り]

     概要を見る

    © 2019 American Society for Microbiology. In various positive-sense single-stranded RNA viruses, a low-fidelity viral RNA-dependent RNA polymerase (RdRp) confers attenuated phenotypes by increasing the mutation frequency. We report a negative-sense single-stranded RNA virus RdRp mutant strain with a mutator phenotype. Based on structural data of RdRp, rational targeting of key residues, and screening of fidelity variants, we isolated a novel low-fidelity mutator strain of influenza virus that harbors a Tyr82-to-Cys (Y82C) singleamino- acid substitution in the PB1 polymerase subunit. The purified PB1-Y82C polymerase indeed showed an increased frequency of misincorporation compared with the wild-type PB1 in an in vitro biochemical assay. To further investigate the effects of position 82 on PB1 polymerase fidelity, we substituted various amino acids at this position. As a result, we isolated various novel mutators other than PB1-Y82C with higher mutation frequencies. The structural model of influenza virus polymerase complex suggested that the Tyr82 residue, which is located at the nucleoside triphosphate entrance tunnel, may influence a fidelity checkpoint. Interestingly, although the PB1- Y82C variant replicated with wild-type PB1-like kinetics in tissue culture, the 50% lethal dose of the PB1-Y82C mutant was 10 times lower than that of wild-type PB1 in embryonated chicken eggs. In conclusion, our data indicate that the Tyr82 residue of PB1 has a crucial role in regulating polymerase fidelity of influenza virus and is closely related to attenuated pathogenic phenotypes in vivo. IMPORTANCE Influenza A virus rapidly acquires antigenic changes and antiviral drug resistance, which limit the effectiveness of vaccines and drug treatments, primarily owing to its high rate of evolution. Virus populations formed by quasispecies can contain resistance mutations even before a selective pressure is applied. To study the effects of the viral mutation spectrum and quasispecies, high- and low-fidelity variants have been isolated for several RNA viruses. Here, we report the discovery of a low-fidelity RdRp variant of influenza A virus that contains a substitution at Tyr82 in PB1. Viruses containing the PB1-Y82C substitution showed growth kinetics and viral RNA synthesis levels similar to those of the wild-type virus in cell culture; however, they had significantly attenuated phenotypes in a chicken egg infection experiment. These data demonstrated that decreased RdRp fidelity attenuates influenza A virus in vivo, which is a desirable feature for the development of safer live attenuated vaccine candidates.

    DOI Scopus

  • Dephosphorylation of clustered phosphoserine residues in human Grb14 by protein phosphatase 1 and its effect on insulin receptor complex formation

    Taira J., Yoshida K., Takemoto M., Hanada K., Sakamoto H.

    Journal of Peptide Science    25 ( 10 )   2019年10月  [査読有り]

     概要を見る

    © 2019 European Peptide Society and John Wiley & Sons, Ltd. The physical interaction of the human growth factor receptor-bound protein 14 (hGrb14) and the insulin receptor (IR) represses insulin signaling. With respect to the recruiting mechanism of hGrb14 to IR respond to insulin stimulus, our previous reports have suggested that phosphorylation of Ser358, Ser362, and Ser366 in hGrb14 by glycogen synthase kinase-3 repressed hGrb14–IR complex formation. In this study, we investigated phosphatase-mediated dephosphorylation of the hGrb14 phosphoserine residues. An in vitro phosphatase assay with hGrb14-derived synthetic phosphopeptides suggested that protein phosphatase 1 (PP1) is involved in the dephosphorylation of Ser358 and Ser362. Furthermore, coimmunoprecipitation experiments suggested that insulin-induced hGrb14–IR complex formation was repressed by the substitution of Ser358 or Ser362 with glutamic acid. These findings suggested that phosphate groups on Ser358 and Ser362 in hGrb14 are dephosphorylated by PP1, and the dephosphorylation facilitates hGrb14–IR complex formation.

    DOI Scopus

  • Hormone-like peptides and small coding genes in plant stress signaling and development

    Takahashi F., Hanada K., Kondo T., Shinozaki K.

    Current Opinion in Plant Biology    51   88 - 95   2019年10月  [査読有り]  [招待有り]

     概要を見る

    © 2019 Elsevier Ltd Recent works have shed light on the long-distance interorgan signaling by which hormone-like peptides precisely regulate physiological effects in a manner similar to phytohormones. Many such peptides have already been identified in the primary model plant, Arabidopsis thaliana. In addition, Arabidopsis genome reanalysis revealed over 7000 novel candidate small coding genes, some of which are likely to be associated with hormone-like peptides. Hormone-like peptides have also been reported to play critical roles in interorgan communications during morphogenesis and stress responses. In this review, we focus on the functional roles of hormone-like peptides and small coding genes in cell-to-cell and/or long-distance communications during plant stress signaling and development and discuss the evolutionary conservation of these peptides among plants.

    DOI Scopus

  • Transcriptome analysis and identification of a transcriptional regulatory network in the response to H<inf>2</inf>O<inf>2</inf>

    Hieno A., Naznin H., Inaba-Hasegawa K., Yokogawa T., Hayami N., Nomoto M., Tada Y., Yokogawa T., Higuchi-Takeuchi M., Hanada K., Matsui M., Ikeda Y., Hojo Y., Hirayama T., Kusunoki K., Koyama H., Mitsuda N., Yamamoto Y.

    Plant Physiology    180 ( 3 ) 1629 - 1646   2019年07月  [査読有り]

     概要を見る

    © 2019 American Society of Plant Biologists. Hydrogen peroxide (H2O2) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H2O2 in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings. Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses in terms of environmental stresses and between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling. Quantitative analysis revealed that ABA accumulation is induced by H2O2 but SA is not, suggesting that the implied crosstalk with ABA is achieved through ABA accumulation while the crosstalk with SA is different. We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes based on the time-course transcriptome analysis for the H2O2 response, in vivo regulation of the regulated TF by the regulator TF identified by expression analysis of mutants and overexpressors, and in vitro binding of the regulator TF protein to the target TF promoter. These analyses enabled the establishment of part of the transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported. This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis.

    DOI Scopus

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学術関係受賞 【 表示 / 非表示

  • 研究奨励賞 「網羅的なゲノム情報を利用した進化ゲノミクス研究」

    2014年06月   日本進化学会   日本国

    受賞者:  花田耕介

  • 九州工業大学職員 SS評価に基づく表彰

    2014年03月06日   九州工業大学   日本国

    受賞者:  花田耕介

  • 研究奨励賞 「Breakthrough in evolutionary mechanism of duplicate genes based on bioinformatics」

    2012年03月   理化学研究所   日本国

    受賞者:  花田 耕介

科研費獲得実績 【 表示 / 非表示

  • シロイヌナズナ生態株間の二次代謝産物量をコントロールするncRNA遺伝子の役割

    若手研究(A)

    研究期間:  2013年04月  -  2016年03月

    研究課題番号:  25710017

  • アルタナリア病原菌の植物寄生性を決定するCD染色体の進化的起源と成立機構

    基盤研究(A)

    研究期間:  2011年04月  -  2015年03月

    研究課題番号:  23248007

 

担当授業科目 【 表示 / 非表示

  • 2019年度  生物学Ⅰ

  • 2019年度  専門概要

  • 2019年度  ゲノム生物学特論

  • 2018年度  生物学Ⅰ

  • 2018年度  専門概要

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