2022/09/21 更新

クメムラ モモコ
久米村 百子
KUMEMURA Momoko
Scopus 論文情報  
総論文数: 0  総Citation: 0  h-index: 8

Citation Countは当該年に発表した論文の被引用数

所属
大学院生命体工学研究科 生体機能応用工学専攻
職名
准教授
外部リンク

取得学位

  • 東京都立大学  -  博士(理学)   2005年03月

学内職務経歴

  • 2018年04月 - 現在   九州工業大学   大学院生命体工学研究科   生体機能応用工学専攻     准教授

論文

  • Single Cell Classification Using Statistical Learning on Mechanical Properties Measured by MEMS Tweezers(共著) 査読有り

    Ahmadian B., Mbujamba D., Gerbedoen J.C., Kumemura M., Fujita H., Collard D., Dabo-Niang S., Lagadec C., Tarhan M.C.

    IEEE Symposium on Mass Storage Systems and Technologies   2022-January   317 - 320   2022年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Cell population is heterogenous and so presents a wide range of properties as metastatic potential. But using rare cells for clinical applications requires precise classification of individual cells. Here, we propose a multi-parameter analysis of single cells to classify them using statistical learning techniques and to predict the sub-population of each cell, although they may have close characteristics. We used MEMS tweezers to analyze mechanical properties (stiffness, viscosity, and size) of single cells from two different breast cancer cell lines in a controlled environment and run supervised learning methods to predict the population they belong to. This label-free method is a significant step forward to distinguish rare cell sub-populations for clinical applications.

    DOI: 10.1109/MEMS51670.2022.9699466

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  • Fabricating silicon resonators for analysing biological samples(共著) 査読有り 国際誌

    Kumemura M., Pekin D., Menon V.A., Van Seuningen I., Collard D., Tarhan M.C.

    Micromachines   12 ( 12 )   2021年12月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:記事・総説・解説・論説等(学術雑誌)

    The adaptability of microscale devices allows microtechnologies to be used for a wide range of applications. Biology and medicine are among those fields that, in recent decades, have applied microtechnologies to achieve new and improved functionality. However, despite their ability to achieve assay sensitivities that rival or exceed conventional standards, silicon-based microelectromechanical systems remain underutilised for biological and biomedical applications. Although microelectromechanical resonators and actuators do not always exhibit optimal performance in liquid due to electrical double layer formation and high damping, these issues have been solved with some innovative fabrication processes or alternative experimental approaches. This paper focuses on several examples of silicon-based resonating devices with a brief look at their fundamental sensing elements and key fabrication steps, as well as current and potential biological/biomedical applications.

    DOI: 10.3390/mi12121546

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  • 細胞の力学特性と遺伝子発現情報の相関関係取得のためのMEMSピンセット

    久米村百子, 金田祥平, 藤田博之

    月刊 細胞 ( ニューサイエンス社 )   52 ( 13 )   42 - 43   2020年11月

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    担当区分:筆頭著者   記述言語:日本語   掲載種別:記事・総説・解説・論説等(商業誌、新聞、ウェブメディア)

  • Nanopin - A MEMS Based Sensor for the Analysis of Single Cell Mechanical Properties (共著) 査読有り 国際誌

    Kumemura M., Kudo L.C., Ma Z., Karsten S.L.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   2020-January   311 - 314   2020年01月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Canada   Vancouver   2020年01月18日  -  2020年01月22日

    © 2020 IEEE. We developed a MEMS based sensor, Nanopin, for mechanical characterization of individual adherent cells. Nanopin consists of sensing tip that forms a contact with a cell, a displacement sensor, and an actuator. The feasibility of sensing was evaluated using various concentrations of agarose gel, and then the stiffness measurements of human carcinoma cells were conducted on different surfaces. After the measurements of cells, we confirmed that cells grow normally in an incubator.

    DOI: 10.1109/MEMS46641.2020.9056113

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  • Real-time measurement of the physical properties of DNA-ligand complexes (共著) 査読有り 国際誌

    Pekin D., Perret G., Kumemura M., Jalabert L., Meignan S., Fujita H., Collard D., Tarhan M.C.

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   892 - 893   2019年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Switzerland   Basel   2019年10月27日  -  2019年10月31日

    © 2019 CBMS-0001. This paper reports an original approach to assess the effects of DNA binding agents on the physical properties of DNA in nucleus-like conditions. We are using Silicon Nanotweezers (SNT), to monitor the stiffness of phage-λ DNA through increasing concentrations of Doxorubicin, SybrGreen and Hoechst in real-time. We show that Doxorubicin and Hoechst lead to an increase in the stiffness of the DNA until a critical concentration where the hydrogen bonds between the complementary base pairs are perturbed, causing the denaturation of the molecule and reducing the stiffness of the structure, while the SybrGreen decreases the stiffness.

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  • Boyden chamber-based compartmentalized tumor spheroid culture system to implement localized anticancer drug treatment (共著) 査読有り 国際誌

    Shohei Kaneda , Jiro Kawada, Marie Shinohara, Momoko Kumemura, Ryohei Ueno, Tomoaki, Kawamoto, Kenji Suzuki, Beomjoon Kim , Yoshiho Ikeuchi , Yasuyuki Sakai, Dominique Collard, Hiroyuki Fujita, and Teruo Fujii

    Biomicrofluidics ( AIP Publishing )   13   054111   2019年10月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1063/1.5125650

  • Optimization of Surface Treatment of MEMS Probes for Single-cell Capture and Release(共著) 査読有り

    41. K. Hayashi,M. Kumemura, S. Kaneda, V. Menon, L. Jalabert, S. Tachikawa, M. C. Tarhan, T. Fujii, B.- J. Kim, H. Fujita

    Sensors and Materials ( Myu Scientific Publishing )   31 ( 9 )   2873 - 2881   2019年09月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

  • Direct measurement of the mechanical properties of a chromatin analog and the epigenetic effects of para-sulphonato-calix[4]arene (共著) 査読有り 国際誌

    Tauran Y., Tarhan M., Mollet L., Gerves J., Kumemura M., Jalabert L., Lafitte N., Byun I., Kim B., Fujita H., Collard D., Perret F., Desbrosses M., Leonard D., Goutaudier C., Coleman A.

    Scientific Reports   2019年04月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    © 2018 The Author(s). The couple Calix[4]arene-1,3-O-diphosphorous acid (C4diP) and zinc ions (Zn2+) acts as a synergistic DNA binder. Silicon NanoTweezer (SNT) measurements show an increase in the mechanical stiffness of DNA bundles by a factor of >150, at Zn2+ to C4diP ratios above 8, as compared to Zinc alone whereas C4diP alone decreases the stiffness of DNA. Electroanalytical measurements using 3D printed devices demonstrate a progression of events in the assembly of C4diP on DNA promoted by zinc ions. A mechanism at the molecular level can be deduced in which C4diP initially coordinates to DNA by phosphate-phosphate hydrogen bonds or in the presence of Zn2+ by Zn2+ bridging coordination of the phosphate groups. Then, at high ratios of Zn2+ to C4diP, interdigitated dimerization of C4diP is followed by cross coordination of DNA strands through Zn2+/C4diP inter-strand interaction. The sum of these interactions leads to strong stiffening of the DNA bundles and increased inter-strand binding.

    DOI: 10.1038/s41598-019-42267-x

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  • Supramolecular epigenetics: New vistas on anti-bacterial genotoxicity, crystal growth and polymorphism (共著) 査読有り

    Coleman A.W., Kumemura M., Jalabert L., Tauran Y., Perret F., Fujita H.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   4   2261 - 2264   2018年11月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Taiwan   Kaohsiung   2018年11月11日  -  2018年11月15日

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. The development of Silicon Nano-Tweezers (SNTs) along with associated micro-fluidics allows the study of how interactions between DNA and external modifiers change the mechanical properties of DNA. This innovative methodology allows direct observation of the effects of Supramolecular Epigenetics, in which bio-active systems interact non-covalently with DNA. Here Silver ions were found to strongly stiffen DNA bundles, microscope studies showed clumping along the DNA. From here micro-tweezers were prepared allowing crystal growth on DNA strands. Small crystals ca 10 μm formed; EDS and X-rays studies imply that these crystals may be a polymorph of the normal form of silver nitrate.

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  • Mechanical effects of a type 2 endonuclease on DNA trapped by silicon nano tweezers at high molecular density (共著) 査読有り

    Tauran Y., Perret G., Jalabert L., Kumemura M., Brioude A., Fujita H., Collard D.

    22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018   2   857 - 859   2018年11月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Taiwan   Kaohsiung   2018年11月11日  -  2018年11月15日

    Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. This paper reports the advantages to use a MEMS based device consisting of Silicon Nano Tweezers (SNTs) for trapping DNA at high molecular density and monitoring its mechanical effects under an enzymatic reaction. DNA digestion assays have been performed with a Type 2 endonuclease either in solution at low DNA density (≈1x105 bp/µm3) or trapped by SNTs at high DNA density (≈1x107 bp/µm3). Unexpectedly, when DNA is trapped between the tweezers tips, the nuclease behaves differently. The enzyme is not able to digest the DNA but increases its stiffness and viscous losses under the crowded environment provided by the SNTs.

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  • 三次元立体露光法を用いて作製したがん細胞浸潤過程観察用マイクロ流体デバイス (共著) 査読有り

    Tachikawa S., Kaneda S., Kumemura M., Sato R., Tsukamoto T., Fujii T., Suzuki T., Fujita H.

    電気学会論文誌E(センサ・マイクロマシン部門誌)   138 ( 9 )   435 - 440   2018年09月

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    記述言語:日本語   掲載種別:研究論文(研究会,シンポジウム資料等)

    © 2018 The Institute of Electrical Engineers of Japan. This paper describes a microfluidic device for observation of cancer cell invasion induced by chemotaxis. The developed device has two main-channels, the channel filled with cell suspension and the channel filled with solution containing chemotactic factor. These channels are connected via tapered orifice structures to capture multiple cancer cells in it to form cell clusters. Observation of cell invasions affected by cell adhesions is possible with this device. The devices were fabricated by using three-dimensional lithography and PDMS (polydimethylsiloxane) molding. Invasion cells leaving the cell clusters and moving to the channel containing chemotactic factors through the orifices were successfully observed on the device.

    DOI: 10.1541/ieejsmas.138.435

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  • Developing a MEMS device with built-in microfluidics for biophysical single cell characterization (共著) 査読有り 国際誌

    Y. Takayama, G. Perret, M. Kumemura, M. Ataka, S. Meignan, S. L. Karsten, H. Fujita, D. Collard, C. Lagadec, M. C. Tarhan

    Micromachines   9 ( 6 )   275   2018年06月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.3390/mi9060275

  • Simultaneous electrical and mechanical characterization of single cells by an integrated device (共著) 査読有り 国際誌

    Takayama Y., Perret G., Kumemura M., Lagadec C., Meignan S., Ataka M., Fujita H., Tarhan M., Collard D.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   2018-January   297 - 300   2018年04月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Northern Ireland   Belfast   2018年01月21日  -  2018年01月25日

    © 2018 IEEE. The mechanical properties of cells have been suggested as alternative biomarkers; however, there is no practical high-throughput and high information content measurement technique applicable for non-adherent cells. To address this problem, we propose a MEMS-based device with an embedded microfluidic channel. Cells flow inside the channel individually and position directly at a sensing area. Then, developed LabVIEW program applies compression cycles to the cell and detects the mechanical properties of the cell in real-time. At the same time, the electrical measurements are performed. Due to direct measurements, we can simplify the experimental setup and improve the throughput compared to the conventional high information content techniques, e.g. AFM. This technique leads to a promising alternative way to identify non-adherent rare cells, e.g. circulating tumor cells and cancer stem cells.

    DOI: 10.1109/MEMSYS.2018.8346544

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  • Elucidating the mechanism of the considerable mechanical stiffening of DNA induced by the couple Zn2+/Calix[4]arene-1,3-O-diphosphorous acid (共著) 査読有り 国際誌

    37. Y. Tauran, M. C. Tarhan, L. Mollet, J. B. Gerves, M. Kumemura, L. Jalabert, N. Lafitte, I. Byun, B. J. Kim, H. Fujita, D. Collard, Florent Perret, Mickael Desbrosses, Didier Leonard, Christelle Goutaudier, AnthonyW.Coleman

    Scientific reports   8   2018年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1038/s41598-018-19712-4

  • Mesure de la dégradation bio-mécanique d’une fibre d’ADN sous l’effet des rayons X thérapeutiques 査読有り

    G. Perret, T. Lacornerie, F. Manca, S. Giordano, M. Kumemura, N. Lafitte, L. Jalabert, M. C. Tarhan, E. Lartigau, F. Cleri, H. Fujita, and D. Collard

    medecine sciences   33   1026 - 1029   2017年12月

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    記述言語:フランス語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1051/medsci/20173312003

  • Mechanical characterization of single cells to distinguish different breast Cancer cells(共著) 査読有り

    G. Perret, Y. Takayama, M. Kumemura, S. Meignan, H. Fujita, C. Lagadec, D. Collard, and M. C. Tarhan

    The 21th International Conference on Miniaturized Systems for Chemistry and Life Sciences   826 - 827   2017年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    USA   Georgia   2017年10月22日  -  2017年10月26日

  • 体内インプラント医療器具を想定した流体振動型エナジー・ハーベスタ 査読有り

    井上聡史, 高橋巧也, 久米村百子, 石橋和徳, 藤田博之, 橋口 原, 年吉 洋

    電気学会論文誌E   137 ( 6 )   152 - 158   2017年06月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

  • A practical single cell analysis method for mechanical characterization of cancer cells 査読有り

    Baëtens T., Perret G., Takayama Y., Kumemura M., Jalabert L., Meignan S., Lagadec C., Hiroyuki F., Collard D., Tarhan M.C.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   608 - 611   2017年02月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    © 2017 IEEE. This study presents a practical method to perform mechanical characterization of cells using Silicon Nano Tweezers (SNT). Integrated with a microfluidic device, SNT tips were inserted in a channel via a side opening. Cells to be characterized were injected to an inlet of the microfluidic channel and withdrawn by a vacuum pump connected to an outlet. Mechanical properties of a cell, e.g. diameter, stiffness and viscous losses, were analyzed after capturing between the SNT tips (a sensing tip and a compressing tip). After characterization, the captured cell was released to capture another cell. Repeated capturing and characterization steps allow us to test several cells and distinguish fixed and live SUM-159-PT cancer cells based on their mechanical properties.

    DOI: 10.1109/MEMSYS.2017.7863481

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  • A fluidic vibrational energy harvester for implantable medical device applications 査読有り

    Inoue S., Takahashi T., Kumemura M., Ishibashi K., Fujita H., Hashiguchi G., Toshiyoshi H.

    IEEJ Transactions on Sensors and Micromachines   137 ( 6 )   152 - 158   2017年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    © 2017 The Institute of Electrical Engineers of Japan. In this paper, we report on a proof-of-concept level energy harvester device that could earn electric power of 0.093 μW/cm2 by the fluidic motion in a PDMS (polydimethylsiloxane) micro fluidic channel placed on a silicon substrate with built-in permanent electrical charges or so-called electrets. Targeting implantable medical devices such as respiratory pace-maker, the heart beat or aspiration is thought to be the source of motion as a final shape of the energy harvesting system.

    DOI: 10.1541/ieejsmas.137.152

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  • An implantable fluidic vibrational energy harvester 査読有り

    Inoue S., Takahashi T., Kumemura M., Fujita H., Toshiyoshi H.

    Journal of Physics: Conference Series   773 ( 1 )   2016年12月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    © Published under licence by IOP Publishing Ltd. Targeting implantable medical devices such as respiratory pace-maker, we have developed a proof-of-concept level energy harvester device that could earn electric power of 44 μW/cm2 by the fluidic motion in a PDMS microchannel placed on a silicon substrate with built-in permanent electrical charges or so-called electrets. The motion of the working fluid will be operated by the heart beat or breathing as a final shape of the energy harvesting system.

    DOI: 10.1088/1742-6596/773/1/012078

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  • DNA Mechanical Characterization inside Biological Buffer by the Double-Actuator Silicon Nano Tweezers, 査読有り

    Gregoire Perret, Mehmet Tarhan, Laurent Jalabert, Momoko Kumemura, Thomas Lacornerie, Eric Lartigau, Fabrizio Cleri, Hiroyuki Fujita, Dominique Collard

    Asia-Pacific Conference of Transducers and Micro-Nano Technology 2016   2016年06月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Japan   Kanazawa   2016年06月26日  -  2016年06月29日

  • Direct electrical and mechanical characterization of in situ generated DNA between the tips of silicon nanotweezers (SNT) 査読有り

    S. L. Karsten, M. Kumemura, L. Jalabert, N. Lafitte, L. C. Kudo, D. Collard and, H. Fujita

    Lab on a Chip   ( 11 )   2099 - 2107   2016年05月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1039/C6LC00309E

  • MANIPULATING DIFFERENT BIOLOGICAL SAMPLES BY SILICON NANOTWEEZERS 査読有り

    Mehmet C Tarhan, Grégoire Perret, Nicolas Lafitte, Momoko Kumemura, Laurent Jalabert, Yuki Takayama, Stanislav L. Karsten, Hiroyuki Fujita and Dominique Collard

    The 8th International Conference on Microtechnologies in Medicine and Biology   2016年04月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    Seoul   Korea   2016年04月20日  -  2016年04月22日

  • 3D humidity imager in micro environment based on DNA conductivity and rigidity measured by silicon nano tweezers 査読有り

    Perret G., Lafitte N., Tarhan M., Jalabert L., Kumemura M., Lacornerie T., Lartigau E., Cleri F., Fujita H., Collard D.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   2016-February   275 - 277   2016年02月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    © 2016 IEEE. This paper reports a novel approach to scan with a high resolution the relative humidity around water in microsized environment. The humidity sensor is composed of a MEMS device, the Silicon Nano Tweezers (SNT) and a DNA bundle. The principle of the measurement is based on the relation between the electro-mechanical properties (conductance and stiffness) of a DNA bundle and the relative humidity. The system is developed to obtain precisely, quickly, and automatically the middle of the aperture of a microfluidic cavity for self-alignment purpose.

    DOI: 10.1109/MEMSYS.2016.7421613

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  • Real-time mechanical characterization of DNA degradation under therapeutic X-rays and its theoretical modeling 査読有り

    Perret G., Lacornerie T., Manca F., Giordano S., Kumemura M., Lafitte N., Jalabert L., Tarhan M., Lartigau E., Cleri F., Fujita H., Collard D.

    Microsystems and Nanoengineering   2   2016年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    © The Author(s) 2016. The killing of tumor cells by ionizing radiation beams in cancer radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. By contrast, the mechanical behaviour of DNA encompassing sequence sensitivity and elastic transitions to plastic responses is much better understood. A novel approach is proposed here based on a micromechanical Silicon Nanotweezers device. This instrument allows the detailed biomechanical characterization of a DNA bundle exposed to an ionizing radiation beam delivered here by a therapeutic linear particle accelerator (LINAC). The micromechanical device endures the harsh environment of radiation beams and still retains molecular-level detection accuracy. In this study, the first real-time observation of DNA damage by ionizing radiation is demonstrated. The DNA bundle degradation is detected by the micromechanical device as a reduction of the bundle stiffness, and a theoretical model provides an interpretation of the results. These first real-time observations pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.

    DOI: 10.1038/micronano.2016.62

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  • Nano systems and devices for applications in biology and nanotechnology 査読有り

    G. Perret, P. Ginet, M.C. Tarhan, A. Baccouche, T. Lacornerie, M. Kumemura, L. Jalabert, F. Cleri, E.F. Lartigau, B.J. Kim, S.L. Karsten, H. Fujita, Y. Rondelez, T. Fujii, D. Collard

    Solid-State Electronics   115   66 - 73   2016年01月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1016/j.sse.2015.08.019

  • Real time mechanical characterization of DNA in liquid during a radiotherapy treatment and its theoretical analysis 査読有り

    Perret G., Lacornerie T., Manca F., Giordano S., Kumemura M., Lafitte N., Jalabert L., Lartigau E., Fujii T., Cleri F., Fujita H., Collard D.

    2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2015   74 - 76   2015年08月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    © 2015 IEEE. We report the first real-time biomechanical measurement of DNA bundle degradation in stable condition when exposed to a therapeutic radiation beam and a theoretical model to describe DNA damages. The Silicon Nano Tweezers and their new microfluidic system endure the harsh environment of radiation beams and still retain molecular-level accuracy. This result paves the way for both fundamental and clinical studies of DNA degradation under radiation for improved tumor treatment.

    DOI: 10.1109/TRANSDUCERS.2015.7180864

    Scopus

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  • Programmable LDEP technology to fabricate versatile master molds for PDMS continuous-flow microfluidic applications 査読有り

    Renaudot R., Fouillet Y., Jalabert L., Kumemura M., Collard D., Fujita H., Agache V.

    Microfluidics and Nanofluidics   16 ( 4 )   701 - 710   2014年04月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    This paper presents an innovative versatile method aiming at rapid fabrication of a master for polydimethylsiloxane (PDMS) molding. This technology is relying on liquid dielectrophoresis electromechanical microfluidic transduction for programmable ultraviolet (UV) glue manipulation. It enables formation of the master in a tailor-made approach, avoiding the need of micromachined structures unlike in conventional methods. The principle is simple: UV glue, while in liquid phase, is actuated onto an array of electrodes patterned on a Si substrate and cured afterward by UV exposure. The silicon chip and the glue microstructures defined atop of it then play the role of a master for PDMS molding. The glue microstructures' shape is hemispherical which is of high interest for many microfluidic applications. This concept is assessed and validated with two different PDMS chip replica designs, both of them illustrating representative applications in continuous microfluidic: a T-junction design for inflow droplet generation and a "Quake" type valve. Lastly, this protocol has shown to be recyclable since the UV glue microstructures once formed can be easily removed by immersion in an acetone bath, such as the chip is reset and can be reprogrammed afterward to build another glue channels geometry. © 2013 European Union.

    DOI: 10.1007/s10404-013-1256-z

    Scopus

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  • Electret-based low power resonator for robust pressure sensor 査読有り

    Mitsuya H., Ashizawa H., Sugiyama T., Kumemura M., Ataka M., Fujita H., Hashiguchi G.

    Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)   717 - 720   2014年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    We have developed a membrane-less pressure sensor based on squeeze-film damping in a 2-μm driving/sensing gap of a silicon ring-shaped resonator. Its sensing range is from sub-atmospheric to over 1MPa; very wide-range pressure measurement is possible with one sensor element. An electret film having the 200-V-bias voltage was incorporated to the resonator; this allows the excitation of the resonator at very low AC voltage. This membrane-less pressure sensor has robust and low power consumption (nW-range) characteristics. © 2014 IEEE.

    DOI: 10.1109/MEMSYS.2014.6765741

    Scopus

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  • Fabrication method of based-paraffin reconfigurable microfluidic chips using liquid dielectrophoresis and electrowetting transductions 査読有り

    Renaudot R., Agache V., Fouillet Y., Kumemura M., Jalabert L., Collard D., Fujita H.

    2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013   2193 - 2196   2013年12月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    A new and original concept aiming at rapid fabrication of continuous microfluidic chips with a channel-based reconfigurable and programmable geometry is reported in this paper. Interfaces between DI water and liquid paraffin-wax are precisely controlled using electromechanical forces onto an array of electrodes, similar to classical digital microfluidic platforms. As the liquid-liquid interfaces define the targeted geometry, the paraffin is solidified by decreasing the chip temperature using an integrated thermoelectric cooler. Then, the paraffin channel-based chip is used for in-flow continuous microfluidic experiments. First results describing the whole concept are shown with typical fluidic geometries. © 2013 IEEE.

    DOI: 10.1109/Transducers.2013.6627238

    Scopus

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  • Active control of silicon nanotweezers detects enzymatic reaction at the molecular level 査読有り

    Lafitte N., Haddab Y., Le Gorrec Y., Guillou H., Kumemura M., Jalabert L., Collard D., Fujita H.

    2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013   2357 - 2360   2013年12月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    This work has achieved the control of micromachined tweezers for the enhancement of the sensing of DNA molecules. The tool allows a routine trapping of the molecules and the real-time monitoring of the mechanical properties of the bundle of molecules. Monitoring the resonance response of the system, the bundle and the interactions of enzymes or proteins are characterized. Furthermore the design and the implementation of state feedback decrease the equivalent stiffness of the system and improve the sensitivity of the tool. © 2013 IEEE.

    DOI: 10.1109/Transducers.2013.6627279

    Scopus

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  • Characterization of Π-conjugated metallopolymer's mechanical stiffness by using silicon nanotweezers 査読有り

    Lee J., Yagi K., Kumemura M., Sato T., Jalabert L., Lafitte N., Collard D., Houjou H., Fujita H.

    2013 Transducers and Eurosensors XXVII: The 17th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS 2013   2684 - 2687   2013年12月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    In this research, we demonstrate for the first time the in-situ polymerization and the mechanical characterization of metallopolymer wires by MEMS Silicon Nanotweezers. Metallopolymers have interesting semiconductor characteristics that can be tuned by polymerization conditions. The reported method allows the experiment of various polymerization conditions with a high versatility such as the metallopolymer properties (electrical, mechanical, and piezoresistive) can be studied and optimized rapidly on demand. © 2013 IEEE.

    DOI: 10.1109/Transducers.2013.6627359

    Scopus

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  • Closed-loop control of silicon nanotweezers for improvement of sensitivity to mechanical stiffness measurement and bio-sensing on DNA molecules 査読有り

    Lafitte N., Haddab Y., Le Gorrec Y., Guillou H., Kumemura M., Jalabert L., Fujita H., Collard D.

    IEEE International Conference on Intelligent Robots and Systems   1022 - 1027   2013年12月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    In this work we show that implementation of closed-loop control to silicon nanotweezers improves the sensitivity of the tool for mechanical characterization of biological molecules. Micromachined tweezers have already been used for the characterization of mechanical properties of DNA molecules [1] as well as for the sensing of enzymatic reactions on DNA bundle [2], [3]. However the resolution of the experiments does not allow the sensing on single molecules. Hereafter we show theoretically and experimentally that, reducing the resonance frequency of the system by the implementation of a state feedback, the sensitivity to stiffness variation is enhanced. Such improvement leads to better resolution for detection of enzymatic reactions on DNA [4]. © 2013 IEEE.

    DOI: 10.1109/IROS.2013.6696476

    Scopus

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  • Silicon Nanotweezers with a microfluidic cavity for the real time characterization of DNA damage under therapeutic radiation beams 査読有り

    Perret G., Chiang P., Lacornerie T., Kumemura M., Lafitte N., Guillou H., Jalabert L., Lartigau E., Fujii T., Cleri F., Fujita H., Collard D.

    Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS   2013年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    We report the biomechanical characterization of λ-DNA bundle exposed to a therapeutic radiation beam by silicon Nanotweezers. The micromechanical device endures the harsh environment of radiation beams, and still retains molecular-level detection accuracy. The real-time DNA bundle degradation is observed in terms of biomechanical stiffness and viscosity reduction, both in air and in solution. These results pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment. © 2013 IEEE.

    DOI: 10.1109/EMBC.2013.6611123

    Scopus

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  • Integrated MEMS platform with silicon nanotweezers and open microfluidic device for real-time and routine biomechanical probing on molecules and cells 査読有り

    Lafitte N., Guillou H., Kumemura M., Jalabert L., Fujii T., Fujita H., Collard D.

    Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS   148 - 151   2013年10月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    This paper describes an integrated biomechanical platform for real-time molecular or cellular assays. This platform is composed of silicon nanotweezers to manipulate the biological samples and an open microfluidic to handle solution and reactive agents. The tweezers are fabricated by standard Silicon-On-Insulator based micromachining processes (2 masks +1 additional mask for special tips) and integrate actuator, trapping tips and sensor. The microfluidic device is produced from common polydimethylsiloxane (PDMS) micromolding and integrates active valves for controlling the biological medium. Combining both technologies, a versatile experimental setup, built up in an enclosed space (< 10 cm3), enables direct interrogation of molecules or cells in solution. The silicon nanotweezers sense slight biological modifications of the trapped molecules or cell by monitoring the mechanical resonance response, which keeps a high Q factor (over 20) in liquid. Biomolecular assays (molecule trapping and enzymatic reaction kinetics) as well as characterizations of cells are reported here. The system provide molecular level resolution and is sensitive enough to capture cell biomechano-transduction activities. Moreover as the system is handy, it may be an easy, fast and quantitative alternative to existing methods. © 2013 IEEE.

    DOI: 10.1109/EMBC.2013.6609459

    Scopus

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  • Alternative method for local surface functionalization using liquid dielectrophoresis: An application with polyethyleneimine polymer for bacteria trapping onto a surface 査読有り

    R. Renaudot, T. Nguyen, Y. Fouillet, D. Jary, M. Kumemura, L. Jalabert, D. Collard, H. Fujita, V. Agache

    RSC Advances   3   9214 - 9218   2013年04月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1039/C3RA40700D

  • Performances of a broad range of dielectric stacks for Liquid Dielectrophoresis transduction 査読有り

    R. Renaudot, V. Agache, Y. Fouillet, M. Kumemura, L. Jalabert, D. Collard, H. Fujita

    Microfluid Nanofluid   15   297 - 307   2013年03月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1007/s10404-013-1156-2

  • Four-terminal electrical measurement of a DNA molecular bundle captured by silicon nanotweezers 査読有り

    M. Usami, T. Ishida, M. Kumemura, L. Jalabert, D. Collard, H. Fujita

    IEEJ Transactions on Sensors and Micromachines   133 ( 3 )   98 - 99   2013年03月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1541/ieejsms.133.98

  • Nano bio research approach by micro technology 査読有り

    D. Collard, S. H. Kim, T. Osaki, M. Kumemura, B. Kim, D. Fourmy, T. Fujii, S. Takeuchi, S. L. Karsten, H. Fujita

    Drug discover today   18 ( 11-12 )   552 - 559   2013年02月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1016/j.drudis.2013.02.002

  • Real time bio mechanical characterization of DNA damage under therapeutic radiation beams 査読有り

    Perret G., Lacornerie T., Kumemura M., Lafitte N., Guillou H., Jalabert L., Lartigau E., Fujii T., Cleri F., Fujita H., Collard D.

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   2   1162 - 1163   2013年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    We report the first real-time biomechanical measurement of DNA bundle degradation in solution when exposed to a therapeutic radiation beam. The Silicon Nano Tweezers and their microfluidic endure the harsh environment of radiation beams and still retain molecular-level accuracy. This result paves the way for both fundamental and clinical studies of DNA degradation under radiation for improved tumor treatment.

    Scopus

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  • Isothermal amplification of DNA on tips of silicon nanotweezers and its electrical and mechanical characterization 査読有り

    Kumemura M., Karsten S., Lafitte N., Guillou H., Jalabert L., Fujita H., Collard D.

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   3   1992 - 1994   2013年01月

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    担当区分:筆頭著者   記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    We performed electrical and mechanical characterization of single stranded DNA (ssDNA) bundle generated on the tips of a micromachined silicon nanotweezers (SNT) via isothermal rolling circle amplification (RCA; Fig 1). Primers with 7 nm length (21 nts) were amplified and final length of amplified product became longer than 2.5 μm. Generated ssDNA bridge between tips which were formed by dielectrophoresis (DEP) was easily visualized and detected via conductivity and resonance frequency (Fres) measurements. These data provides a "proof-of-principle " for the feasibility of the on-chip label free DNA detection device that can be used for a variety of biomedical applications.

    Scopus

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  • Local surface modification at the microscale enabled by liquid dielectrophoresis 査読有り

    Renaudot R., Nguyen T., Fouillet Y., Jalabert L., Kumemura M., Collard D., Fujita H., Agache V.

    17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013   1   155 - 157   2013年01月

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    記述言語:英語   掲載種別:研究論文(国際会議プロシーディングス)

    This paper reports a new protocol on-chip for functionalization purpose enabled by liquid dielectrophoresis (LDEP) technique at the microscale, and its adaptation to the nanoscale for the MEMS/NEMS applications. In a previous study, circular spots (diameter: 25-100 μm), made of polymer, have been accurately positioned onto a surface using LDEP. In this study, doped polysilicon LDEP structures have been fabricated with submicronic electrodes widths, to displace weak amount of liquids and create femtoliter sessile droplets. The final goal consists in dispensing fL droplets localized at the submicronic electrodes tip, and then implementing such structures to address MEMS/NEMS cantilevers local functionalization. Copyright © (2013) by the Chemical and Biological Microsystems Society All rights reserved.

    Scopus

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  • Optimized micro devices for liquid-dielectrophoresis (LDEP) actuation of conductive solutions 査読有り

    R. Renaudot, B. Daunay, M. Kumemura, V. Agache, L. Jalabert, D. Collard, H. Fujita

    Sensors and Actuators B: Chemical   177   620 - 626   2012年11月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1016/j.snb.2012.11.049

  • Enzymatic Reaction in Droplets Manipulated with Liquid Dielectrophoresis 査読有り

    M. Kumemura, D.Collard, S. Yoshizawa, B. Wee, S. Takeuchi, H. Fujita

    ChemPhysChem   13   3308 - 3312   2012年07月

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    担当区分:責任著者   記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1002/cphc.201200354

  • Single DNA molecule trapping with silicon nanotweezers using pulsed dielectrophoresis 査読有り

    M. Kumemura, D. Collard, N. Sakaki, C. Yamahata, M. Hosogi, G. Hashiguchi, H. Fujita

    Journal of Micromechanics and Microengineering   21   054020   2011年12月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.1088/0960-1317/21/5/054020

  • Optimization of LDEP digital microfluidic transduction for biomedical applications 査読有り

    R. Renaudot, V. Agache, B. Daunay, P. Lambert, M. Kumemura, Y. Fouillet, D. Collard, H. Fujita

    Micromachines   2 ( 2 )   258 - 273   2011年06月

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    記述言語:英語   掲載種別:研究論文(学術雑誌)

    DOI: 10.3390/mi2020258

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著書

  • Silicon Nanotweezers for Molecules and Cells Manipulation and Characterization

    Collard D., Lafitte N., Guillou H., Kumemura M., Jalabert L., Fujita H.(共著)

    2015年11月  ( ISBN:9783527337842

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    記述言語:英語

    © 2015 Wiley-VCH Verlag GmbH & Co. KGaA. All rights reserved. In this chapter, Silicon Nanotweezer (SNT) is presented as a generic microdevice allowing bio-mechanical assays of filamentary molecules and cells. SNT operations and design are presented. The chapter explains the SNT microfabrication process; the operations and applications to molecules and cell characterization. Commonly integrated in microsystem designs, folded-beam springs are designed to minimize beams areas, decrease their mechanical stiffness, and enhance displacement ranges. In the current design, three sets of folded-beam suspensions support different parts of the system and provide the electrical connections for the actuation and sensing. In phenomenological models, the dynamic properties inform us on the stiffness and dissipation at the resonance frequency, whereas the static properties are related directly to the force. The monitoring of the resonance properties prevents any damage to the cell or to the SNT. To conclude, the silicon nanotweezers can be applied to handle various objects other than DNA molecules.

    Scopus

口頭発表・ポスター発表等

  • 接着細胞の機械特性計測プロトコルの構築

    浅倉悠斗, Karsten Stanislav L., Kudo Lili C., Ma Zhongcai, 久米村百子

    電気学会センサ・マイクロマシン部門総合研究会, 合同研究会  2022年06月 

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    開催期間: 2022年06月07日 - 2022年06月08日   記述言語:日本語   開催地:石川県金沢市  

  • Stiffness measurements of suspended/adherent cell with MEMS tweezers

    N. Inoue and M. Kumemura

    The 9 th International Symposium on Applied Engineering and Sciences (SAES2021)  2021年12月 

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    開催期間: 2021年12月05日 - 2021年12月08日   記述言語:英語  

  • 細胞の機械特性と細胞骨格タンパク質の遺伝子発現量の相関評価に向けた 単一細胞搬送から解析までの手法検討

    高村公平、久米村百子

    化学とマイクロ・ナノシステム学会第44回研究会  2021年11月 

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    開催期間: 2021年11月09日 - 2021年11月11日   記述言語:日本語  

  • Surface modification on polymethyl methacrylate for 3D printed microfluidic sensor,

    M. Matsuura, A. Coleman, M. Lloret, M. Kumemura

    The 30th anniversary word congress on biosensors  2021年07月 

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    開催期間: 2021年07月26日 - 2021年07月29日   記述言語:英語   開催地:online  

  • MEMSを用いた単一細胞の機械特性計測

    井上敦貴, 久米村百子

    化学とマイクロ・ナノシステム学会第40回研究会  化学とマイクロ・ナノシステム学会

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    開催期間: 2019年11月19日 - 2019年11月21日   記述言語:日本語   開催地:静岡県浜松市アクトシティ浜松  

工業所有権

  • DNA の検出方法

    藤田博之, カルステン スタニスラフ エル, コラール ドミニク, 久米村百子

     詳細を見る

    出願番号:特願2015-543920 (P2015-543920)  出願日:2014年10月24日

    登録番号:特許第6385356号 (P6385356)  登録日:2018年09月05日

担当授業科目(学内)

  • 2021年度   国際インターンシップ

  • 2021年度   半導体トピックセミナー

  • 2021年度   マイクロ分析システム

  • 2020年度   半導体トピックセミナー

  • 2020年度   マイクロ分析システム

  • 2019年度   マイクロ分析システム

▼全件表示

学会・委員会等活動

  • 電気学会   会計会議(部門)委員  

    2022年06月 - 2024年06月

  • 第39回センサ・マイクロマシンと応用システムシンポジウム, 分野7   論文委員  

    2022年02月 - 2022年12月

  • 第38回センサ・マイクロマシンと応用システムシンポジウム, 分野7   論文委員主査, 若手賞表彰委員  

    2021年02月 - 2021年12月