研究者詳細 - 安永　卓生

その他リンク： https://www.jstage.jst.go.jp/article/jcrsj/61/1/61_43/_pdf

Computational strategy for studying structural change of tritium-substituted macromolecules by a beta decay to helium-3 査読有り国際誌

Fujiwara Susumu, Nakamura Hiroaki, Li Haolun, Miyanishi Hisanori, Mizuguchi Tomoko, Yasunaga Takuo, Otsuka Takao, Hatano Yuji, Saito Shinji

日本シミュレーション学会英文誌 ( 一般社団法人日本シミュレーション学会 ) 6 ( 1 ) 94 - 99 2019年01月

記述言語：英語掲載種別：研究論文（学術雑誌）

We propose a computational strategy for investigating structural change of tritium-substituted macromolecules. Effects of radiation on macromolecules such as polymeric materials and DNA are classified into three categories: (1) direct action, (2) indirect action, and (3) decay effect. In this study, we focus on the decay effect exclusively. After a beta decay of substituted tritium in macromolecules to helium-3, the generated inert helium-3 is assumed to be deleted quickly. To get an insight into the decay effect to the damage of macromolecules, we perform molecular dynamics simulations of tritium-deleted macromolecules and analyze their structural change. Preliminary simulation results of decay effect on polymeric materials and DNA are presented.

DOI： 10.15748/jasse.6.94

その他リンク： https://www.jstage.jst.go.jp/article/jasse/6/1/6_94/_pdf

3次元クライオ電子顕微鏡法が解き明かす細胞の運動招待有り査読有り

安永卓生, 荒牧慎二, 肥後智也

顕微鏡 ( 公益社団法人日本顕微鏡学会 ) 53 ( 3 ) 118 - 122 2018年12月

担当区分：筆頭著者,　責任著者記述言語：日本語掲載種別：記事・総説・解説・論説等（学術雑誌）

クライオ電子顕微鏡法は，生理的条件に近い状態で分子構造を明らかにできる強力な手法の1つである．電子線トモグラフィー法と組み合わせることで，細胞や組織内での分子動態をナノレベルの分解能で明らかにすることができる．我々は，細胞運動や細胞センシングにとって重要な構造である糸状仮足のアーキテクチャを明らかにしてきた．力学的バランスから推定される本数のアクチン繊維束のセットが観測され，架橋構造とX線結晶解析法による原子モデルと統合し，束化因子ファシンのアクチンへの結合様式を示した．また，アクチン束周辺のファシンの協働的結合や短いアクチン等のブラウジングの結果として，アクチン細胞骨格の束化メカニズムを提唱した．さらに，糸状仮足先端でのアクチン繊維の振る舞いも明らかにしている．今後，クライオ電子線トモグラフィー法は，生物試料のみならず，燃料電池を始めとする水系の材料分野でも有効であろう．

DOI： 10.11410/kenbikyo.53.3_118

その他リンク： https://search.jamas.or.jp/link/ui/2019210169

Correction: Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport (Scientific Reports DOI: 10.1038/s41598-017-15575-3) 査読有り

Toba S., Jin M., Yamada M., Kumamoto K., Matsumoto S., Yasunaga T., Fukunaga Y., Miyazawa A., Fujita S., Itoh K., Fushiki S., Kojima H., Wanibuchi H., Arai Y., Nagai T., Hirotsune S.

Scientific Reports ( Scientific Reports ) 8 ( 1 ) 2018年12月

掲載種別：記事・総説・解説・論説等（大学・研究所紀要）

© 2018 The Author(s). In this Article, Figure 5 was inadvertently published as Figures 5 and 6, leading to the incorrect publication of Figure 6 as Figure 7 and the omission of the correct Figure 7. The correct Figures 5, 6, and 7 appear below as Figures 1, 2, and 3 respectively. The Figure legends are correct. (Figure Presented).

DOI： 10.1038/s41598-018-25979-4

その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047096548&origin=inward

高大接続改革と九州工大

安永卓生

明専会報 ( 明専会 ) 2018年07月

Effects of mixed ultrafine colloidal silica particles on chemical mechanical polishing of sapphire 査読有り

Bun-Athuek Natthaphon, Takazaki Hiroko, Yoshimoto Yutaka, Khajornrungruang Panart, Yasunaga Takuo, Suzuki Keisuke

Jpn. J. Appl. Phys. 57 ( 7 ) 2018年06月

記述言語：英語掲載種別：研究論文（学術雑誌）

The effects of ultrafine colloidal silica particles adsorbed on the surfaces of large silica particles in slurries used for the chemical mechanical polishing of sapphire were studied. Sapphire wafers were polished using hybrid silica particles that were composed of a mixture of ultrafine (4 nm) colloidal silica particles and large (20, 55, and 105 nm) silica particles. Dynamic light scattering results showed that the hybrid particles became larger after mixing. Transmission electron microscopy images revealed that the ultrafine particles adhered to the surfaces of the large particles and that the surface shape of the hybrid particles were changed by the mixing process. Atomic force microscopy and polishing results showed that the surface roughnesses and material removal rates of sapphire substrates were improved by using the hybrid particles. The coefficient of friction between the surface of the sapphire substrate and the hybrid particles was higher than that obtained using single-sized particles. These results confirm that the hybrid silica particles enhanced the performance of sapphire polishing.

DOI： 10.7567/JJAP.57.07MD03

その他リンク： https://ci.nii.ac.jp/naid/150000116077

生命を解き明かすクライオ電子顕微鏡法の新時代招待有り査読有り

安永卓生

顕微鏡 ( 公益社団法人日本顕微鏡学会 ) 53 ( 1 ) 2 2018年04月

クライオ電子顕微鏡法を支える画像解析と三次元像構築ーその原理と最近のソフトウェアの発展

安永卓生, 荒牧慎二

実験医学 ( 羊土社 ) 36 ( 8 ) 2018年04月

生命を解き明かすクライオ電子顕微鏡法の新時代

安永卓生

顕微鏡 ( 日本顕微鏡学会 ) 1 ( 53 ) 2018年04月

高大接続改革と九州工大 ―学生とのよりよいマッチングを求めて　類別入試と学部・学科の改組 ―

安永卓生

明専会報 ( 明専会 ) 2018年01月

難加工材料におけるハイブリッド研磨微粒子の最適構造に関する研究:化学的作用と機械的作用に関する考察査読有り

鈴木恵友, Bun-Athuek Natthaphon, 髙﨑寛子, 安永卓生, 吉本裕, パナートカチョーンルンルアン

精密工学会学術講演会講演論文集 ( 公益社団法人精密工学会 ) 2018 ( 0 ) 312 - 313 2018年01月

記述言語：日本語掲載種別：研究論文（学術雑誌）

本研究ではコア微粒子上に直径4nmのナノシリカ微粒子や水酸化フラーレンを吸着により生成したハイブリット研磨微粒子を難加工研磨技術への適用を試みた．ここではコアシリカ微粒子の粒子径やナノシリカ微粒子水酸化フラーレンの吸着状態によりサファイアCMPの材料除去レートが変化した．そのためハイブリット研磨微粒子の構造を最適化することにより，化学的作用や機械的作用を独立に制御する手法を試みたので報告する．

DOI： 10.11522/pscjspe.2018A.0_312

その他リンク： https://ci.nii.ac.jp/naid/130007602798

Alpha-synuclein facilitates to form short unconventional microtubules that have a unique function in the axonal transport 査読有り国際誌

Toba S., Jin M., Yamada M., Kumamoto K., Matsumoto S., Yasunaga T., Fukunaga Y., Miyazawa A., Fujita S., Itoh K., Fushiki S., Kojima H., Wanibuchi H., Arai Y., Nagai T., Hirotsune S.

Scientific Reports 7 ( 1 ) 2017年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

© 2017 The Author(s). Although α-synuclein (αSyn) has been linked to Parkinson's disease (PD), the mechanisms underlying the causative role in PD remain unclear. We previously proposed a model for a transportable microtubule (tMT), in which dynein is anchored to a short tMT by LIS1 followed by the kinesin-dependent anterograde transport; however the mechanisms that produce tMTs have not been determined. Our in vitro investigations of microtubule (MT) dynamics revealed that αSyn facilitates the formation of short MTs and preferentially binds to MTs carrying 14 protofilaments (pfs). Live-cell imaging showed that αSyn co-transported with dynein and mobile βIII-tubulin fragments in the anterograde transport. Furthermore, bi-directional axonal transports are severely affected in αSyn and γSyn depleted dorsal root ganglion neurons. SR-PALM analyses further revealed the fibrous co-localization of αSyn, dynein and βIII-tubulin in axons. More importantly, 14-pfs MTs have been found in rat femoral nerve tissue, and they increased approximately 19 fold the control in quantify upon nerve ligation, indicating the unconventional MTs are mobile. Our findings indicate that αSyn facilitates to form short, mobile tMTs that play an important role in the axonal transport. This unexpected and intriguing discovery related to axonal transport provides new insight on the pathogenesis of PD.

DOI： 10.1038/s41598-017-15575-3

その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85035338808&origin=inward

Katanin p80, NuMA and cytoplasmic dynein cooperate to control microtubule dynamics 査読有り

Jin M., Pomp O., Shinoda T., Toba S., Torisawa T., Furuta K., Oiwa K., Yasunaga T., Kitagawa D., Matsumura S., Miyata T., Tan T., Reversade B., Hirotsune S.

Scientific Reports 7 2017年01月

記述言語：英語掲載種別：研究論文（学術雑誌）

© The Author(s) 2017. Human mutations in KATNB1 (p80) cause severe congenital cortical malformations, which encompass the clinical features of both microcephaly and lissencephaly. Although p80 plays critical roles during brain development, the underlying mechanisms remain predominately unknown. Here, we demonstrate that p80 regulates microtubule (MT) remodeling in combination with NuMA (nuclear mitotic apparatus protein) and cytoplasmic dynein. We show that p80 shuttles between the nucleus and spindle pole in synchrony with the cell cycle. Interestingly, this striking feature is shared with NuMA. Importantly, p80 is essential for aster formation and maintenance in vitro. siRNA-mediated depletion of p80 and/or NuMA induced abnormal mitotic phenotypes in cultured mouse embryonic fibroblasts and aberrant neurogenesis and neuronal migration in the mouse embryonic brain. Importantly, these results were confirmed in p80-mutant harboring patient-derived induced pluripotent stem cells and brain organoids. Taken together, our findings provide valuable insights into the pathogenesis of severe microlissencephaly, in which p80 and NuMA delineate a common pathway for neurogenesis and neuronal migration via MT organization at the centrosome/spindle pole.

DOI： 10.1038/srep39902

その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85009442774&origin=inward

Lis1 restricts the conformational changes in cytoplasmic dynein on microtubules（共著）査読有り

S Toba, K Koyasako, T Yasunaga, S Hirotsune

Microscopy 64 ( 6 ) 419 - 427 2015年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Cytoplasmic dynein is a microtubule-based motor protein that transports intracellular cargo and performs various functions during cell division. We previously reported that Lis1 suppressed dynein motility on microtubules in an idling state. Recently, a model showed that Lis1 prevents the ATPase domain of dynein from transmitting a detachment signal to its micro- tubule-binding domain. However, conformational information on dynein is limited. We used electron microscopy to investigate the conformation of dynein and nucleotide-induced conformational changes on microtubules. The conformation of dynein differed depending on the presence or absence of a nucleotide. In the presence of the nucleotide ADP-vanadate, dynein displayed an extended form on microtubules (extended form), whereas in the absence of a nucleotide, dynein lay along microtubules (compact form). This conformational change reflects chemomechanical coupling in dynein walking on microtubules. We also found that Lis1 fixed the conformation of dynein in the compact form regardless of the nucleotide condition. Removal of the Lis1 dimerization motif abolished Lis1-dependent fixation of dynein in the compact form. This suggests that the idling state of dynein on microtubules induced by Lis1 occurs through the Lis1-dependent arrest of dynein chemomechanical coupling.

DOI： 10.1093/jmicro/dfv055

その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84952772401&origin=inward

問題を解決し続ける生物と工学への玄妙の関

安永卓生

生物物理 ( 生物物理学会 ) 2015年09月

GM130 is a parallel tetramer with a flexible rod-like structure and N-terminally open (Y-shaped) and closed (I-shaped) conformations 査読有り

Ryuichi Ishida, Akitsugu Yamamoto, Kazuhisa Nakayama, Miwa Sohda, Yoshio Misumi, Takuo Yasunaga, Nobuhiro Nakamura

FEBS Journal ( Wiley ) 282 ( 11 ) 2232 - 2244 2015年04月

問題を解決し続ける生物と工学への玄妙の関招待有り査読有り

安永卓生

生物物理 ( 一般社団法人日本生物物理学会 ) 55 ( 5 ) 231 - 231 2015年01月

Transformation from Multi- to Single-lamellar Vesicle by Addition of a Cationic Lipid to 1,2-Dilauroyl-sn-glycero-3-phosphocholine Explored with SAXS and TEM 査読有り国際誌

Kubo Genki, Sakamoto Shunsuke, Fujii Shota, Sanada Yusuke, Yasunaga Takuo, Takahara Atsushi, Sakurai Kazuo

Chemistry Letters ( 公益社団法人日本化学会 ) 43 ( 11 ) 1785 - 1787 2014年01月

記述言語：英語掲載種別：研究論文（学術雑誌）

1,2-Dilauroyl-sn-glycero-3-phosphocholine (DLPC) is one of the major lipids constituting cellular membranes. When DLPC itself is dispersed in aqueous solutions, multilamellar vesicles are formed, although the cellular membranes are made of single bilayers. We found that the addition of a calix[4]arene derivative (CaL[4]C3) bearing four amine groups as the headgroup to the DLPC multilamellar vesicle induced the structural transformation to a single-lamellar vesicle, by use of small-angle X-ray scattering and electron microscopy, indicating that the highly condensed ionic groups of CaL[4]C3 are useful to control membrane morphology.

DOI： 10.1246/cl.140668