FMP30 is required for the maintenance of a normal cardiolipin level and mitochondrial morphology in the absence of mitochondrial phosphatidylethanolamine synthesis. Reviewed

Kuroda T,Tani M,Moriguchi A,Tokunaga S,Higuchi T,Kitada S,Kuge O.

Mol. Microbiol.   80   2011.02

Mega Assemblages of Oligomeric Aerolysin-like Toxins Stabilized by Toxin-associating Membrane Proteins Reviewed

Shimada,H,Kitada S.

J. Biocem.   149   103 - 115   2011.01

Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells. Reviewed

Akiba T.,Abe Y.,Kitada S.,Kusaka Y.,Ito A.,Ichimatsu T.,Katayama H.,Akao T.,Higuchi K.,Mizuki E.,Ohba M.,Kanai R.,Harata K.

J. Mol. Biol   386   121 - 133   2009.04

Purification and characterization of human phosphatidylserine synthase 1 and 2. Reviewed

Tomohiro S.,Kawaguti A.,Kawabe Y.,Kitada S.,and Kuge O.

Biochem. J.   418   421 - 429   2009.04

Parasporin-2 requires GPI-anchored proteins for the efficient cytocidal action to human hepatoma cells. Reviewed

Kitada,S.,Yuichi Abe,Maeda,T.,and Shimada H.

Toxicology   264   80 - 88   2009.04

毒素が薬に食物に？　生命科学への応用について

北田　栄、嶋田擴靖

生物の科学 遺伝 ( エヌ・ティー・エス出版 )   2009.03

Raft-targeting and Oligomerization of Parasporin-2, a Bacillus thuringiensis Crystal Protein with Anti-tumor Activity Reviewed

Abe,Y.,Shimada,Y.,and Kitada,S.

J. Biochem.   143   269 - 275   2008.04

Spatial orientation of mitochondrial processing peptidase and a preprotein revealed by fluorescence resonance energy transfer Reviewed

Tomonori G. Nishino,T. G.,Katsuhiko. K.,Kojima,K.,Ogishima,T.,Ito,A.,and Kitada,S.

J. Biochem   141   889 - 895   2007.04

A Protein from a Parasitic Microorganism, Rickettsia prowazekii, Can Cleave the Signal Sequences of Proteins Targeting Mitochondria. Reviewed

Kitada,S.,Uchiyama,T.,Funatsu,T.,Kitada,Y.,Ogishima,T.,and Ito,A.

J Bacteriol.   189   844 - 850   2007.04

Cytocidal Actions of Parasporin-2, an Anti-tumor Crystal Toxin from Bacillus thuringiensis. Reviewed

Kitada,S.,Abe,Y.,Shimada,H.,Kusaka,K.,Matsuo,M.,Katayama,H.,Okumura,S.,Akao,T.,Mizuki,E.,Kuge,O.,Sasaguri,Y.,Ohba,M.,and Ito A.

J. Biol. Chem.   281   26350 - 26360   2006.04

Molecular identification and cytocidal action of parasporin, a protein group of novel crystal

2005.11

Bacillus thuringiensis から発見された新しい結晶性毒素タンパク質（パラスポリン２）

北田 栄、阿部雄一、嶋田擴靖、片山秀樹、赤尾哲之、水城英一、大庭道夫、久下 理、伊藤明夫

第５２回毒素シンポジウム ( 毒素シンポジウム )   2005.07

Recognition and processing of a nuclear-encoded polyprotein precursor by mitochondrial processing peptidase. Reviewed

Oshima,T.,Yamasaki,E.,Ogishima,T.,Kadowaki,K.,Ito A.,and Kitada S.

Biochem. J.   385   55 - 761   2005.04

Molecular identification and cytocidal action of parasporin, a protein group of novel crystal toxins targeting to human cells Reviewed

Sakae Kitada,Yuichi Abe,Akio Ito,Osamu Kuge,Testuyuki Akao,Eiichi Mizuki,and Michio Ohba

Proceedings of the 6th Pacific Rim Conference. In Biotechnology of Bacillus thuringiensis and Its Environmental Impact., Victria BC 23-27 (2005) review (Côtè, J.-C., Otvos, I.S., Schwartz, J.-L. and Vincent, C. eds.).   2005.04

Oligomerization of parasporin-2, a new crystal protein from non-insecticidal Bacillus thuringiensis, in lipid rafts. Reviewed

Abe,Y.,Kitada S.,Kuge,O.,Ohba M.,and Ito,A

Proceedings of the 6th Pacific Rim Conference. In Biotechnology of Bacillus thuringiensis and Its Environmental Impact., Victria BC(Côtè, J.-C., Otvos, I.S., Schwartz, J.-L. and Vincent, C. eds.).   2005.04

Crystallization of parasporin-2, a Bacillus thuringiensis crystal protein with selective cytocidal activity against human cells. Reviewed

Akiba,T.,Abe,Y.,Kitada,S.,Kusaka,Y.,Ito,A.,Ichimatsu T.,KatayamaH.,AkaoT.,Higuch,K.,Mizuki E.,Ohba M.,Kanai R.,and Harata K.

Acta Cryst. D   60   2355 - 2357   2004.04

A Bacillus thuringiensis Crystal Protein with Selective Cytocidal Action to Human Cells. Reviewed

Ito,A.,Sasaguri,Y.,Kitada,S.,Kusaka,Y.,Kuwano,K.,Masutomi,K.,Mizuki,E.,Akao,T.,Ohba,M.

J. Biol. Chem.   279   21282 - 21286.   2004.04

Molecular Recognition and Clea vage of Presequence by Mitochondrial Processing Peptidase

2003.12

Determination of the cleavage site of the presequence by mitochondrial processing peptidase on the substrate binding scaffold and the multiple-subsite inside a molecular cavity Reviewed

Kitada S.,Yamasaki E.,Kojima K.,Ito A.

J. Biol. Chem   vol278   pp1879 - 1885.   2003.04

Molecular Recognition and Cleavage of Presequence by Mitochondrial Processing Peptidase Reviewed

Seikagaku   vol. 75 pp1439-1444   2003.04

ＲＩ標識で観るミトコンドリアタンパク質の一生　～成熟編～

北田　栄

九州大学ＲＩセンターニュース ( 九州大学ＲＩセンター )   2003.03

A proposed common structure of substrates bound to mitochondrial processing peptidase. Reviewed

Kojima K.,Kitada S.,Ogishima T.,and Ito A.

J. Biol. Chem.   276   2115 - 2121   2001.04

Glu(191) and Asp(195) in rat mitochondrial processing peptidase beta subunit are involved in effective cleavage of precursor protein through interaction with the proximal arginine. Reviewed

Kitada S.,Kojima K.,Ito A.

Biochem. Biophys. Res. Commun.   .. 287   594 - 599   2001.04

Electrostatic recognition of matrix targeting signal by mitochondrial Processing Peptidase Reviewed

Journal of Biochemistry   129   155 - 161   2001.04

Mitochondrial Processing Peptidase Reveal the Mode for Specific Cleavage of Import Signal Sequence Reviewed

Taylor A. B.,Smith B. S.,Kitada S.,Kojima K.,Miyaura H.,Otwinowski Z.,Ito A.,Deisenhofer J.

Structure   9   615 - 625   2001.04

Characterization of rat TOM40, a central component of the preprotein translocase of the mitochondrial outer membrane. Reviewed

Suzuki H.,Okazawa Y.,Komiya T.,Saeki K.,Mekada E.,Kitada S.,Ito A.,Mihara K.

J. Biol. Chem.   275   37930 - 37936   2000.04

Glycine-rich Region of Mitochondrial Processing Peptidase α-Subunit is Essential for Binding and Cleavage of the Precursor Proteins Reviewed

Nagao Y.,Kitada S.,Kojima K.,Toh H.,Kuhara S.,Ogishima T.,Ito A.

The Journal of Biological Chemistry   275   34552 - 34556   2000.04

Cooperative Formation of Substrate-Binding Pocket by α-and β-subunit of Mitochondrial Processing Peptidase Reviewed

Kojima K.,Kitada S.,Shimokata K.,Ogishima T.,Ito A.

The Journal of Biological Chemistry   273   25158 - 15163   1998.04

Glutamate Residues Required for Substrate Binding and Cleavage Activity in Mitochondrial Processing Peptidase Reviewed

Kitada S.,Kojima K.,Shimokata K.,Ogishima T.,Ito A.

The Journal of Biological Chemistry   273   32547 - 32553   1998.04

Role of α-Subunit of Mitochondrial Processing Peptidase in Substrate Recogrition Reviewed

Shimokata K.,Kitada S.,Ogishima T.,Ito A.

The Journal of Biological Chemistry   273   25158 - 25163   1998.04

ミトコンドリアタンパク質のプロセシング　プロテオリシス「タンパク質分解の分子機構と

伊藤明夫、荻島　正、北田　栄

蛋白質 核酸 酵素 ( 共立出版 )   1997.05

Substrate recognition by mitochondrial processing peptidase toward the malate dehydrogenase precursor. Reviewed

Shimokata K.,Nishio T.,Song M.C.,Kitada S.,Ogishima T.,and Ito A.

122   2362 - 2367   1997.04

Recognition of the Precursor Proteins by the Mitochondrial Processing Peptidase Reviewed

Ito,A.,Ogishima,T.,Niidome,T.,Kitada,S.,Shimokata,K.

"Proteolysis and Cell Functions" (ed. by Hopsu-Havu et al.) IOS Press   332 - 339   1997.04

Role of basic amino acids in the cleavage of synthetic peptide substrates by mitochondrial processing peptidase. Reviewed

Song M.C.,Shimokata K.,Kitada S.,Ogishima T.,Ito A.

J. Biochem.   120   1163 - 1166   1996.04

Analysis of elements in the substrate required for processing by mitochondrial processing peptidase. Reviewed

Ogishima T.,Niidome T.,Shimokata K.,Kitada S.,Ito A.

J. Biol. Chem.   270   30322 - 30326   1995.04

A Putative Metal-Binding Site in the β Subunit of Rat Mitochondrial Processing Peptidase Is Essential for Its Catalytic Activity Reviewed

Kitada S.,Shimokata K.,Niidome T.,Ogishima T.,Ito A.

Journal of Biochemistry   117   1148 - 1150   1995.04

The Structure of Extension Peptide Recognized by Mitochondrial Processing Peptidase. Reviewed

Niidome,T.,Kitada,S.,Shimokata,K.,Ogishima T,Ito A.

Peptide Chemistry   65 - 68   1995.04

Rat Liver Mitochondrial Processing Peptidase: Primary Structure and Putative Active Site. Reviewed

Kitada S.,Shimokata K.,Song M.C.,Niidome T.,Ogishima T.,Ito A.

"Frontiers in Protein Chemistry and Biotechnology" (ed. by Wang Zongmu and Li Wei) CPH Press   193 - 197   1995.04

Structural requirement for recognition of the precursor proteins by the mitochondrial processing peptidase. Reviewed

Ou W.J.,Kumamoto T.,Mihara K.,Kitada S.,Niidome T.,Ito A.,Omura T.

J. Biol. Chem.   269   24673 - 24678   1994.04

Arginine residues in the extension peptide are required for cleavage of a precursor by mitochondrial processing peptidase. Demonstration using synthetic peptide as a substrate. Reviewed

Niidome T.,Kitada S.,Shimokata K.,Ogishima T.,Ito A.

J. Biol. Chem.   269   24719 - 24722   1994.04

Molecular cloning of the Smaller Subunit(P52)of Rat Liver Mitochondrial Processing Protease. Reviewed

Biochemical and Biophysical Research Communications   190 - 190   1993.04

抗がん性タンパク質パラスポリン2のマウスに対する毒性の解明

木村雄作、北田有美子、日下雅友、北田　栄

第5回パラスポリン研究会 

ヒトすい臓がん細胞に作用する新規パラスポリン産生Bt菌株のスクリーニング

狩野貴之、鮫島結香、斉藤浩之、水城英一、北田 栄

第5回パラスポリン研究会 

効率的なBt菌判別法の検討と新規のBt菌株の探索

前田健佑、鮫島結香、齊藤浩之、水城英一、北田　栄

第84回日本生化学会大会 

抗がん性タンパク質パラスポリン-2のマウスに対する毒性の解明

木村雄作、北田有美子、日下雅友、北田　栄

第84回日本生化学会大会 

がん移植マウスに対するparasporin-2の抗がん作用

麻崎一哉、北田有美子、日下雅友、北田　栄

第84回日本生化学大会  

抗がん性微生物毒素Parasporin-2のKLN206がん移植マウスにおける生体内挙動

未入力 

パラスポリン-2の結腸がん細胞移植マウスに対する抗腫瘍効果

第4回パラスポリン研究会 

Mega complex of anti-tumor cry toxin parasporin-2 in hepatoma cell membrane.

6TH Annual Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Enviromental Impact  

北田　栄,新奇細胞毒素を利用したBt菌バイオ研究新領域への総合展開

特定領域研究「統合がん」報告会 

膜孔形成オリゴマー化毒素は細胞膜で巨大な毒素複合体を形成する

第30回日本分子生物学会年会・第80回日本生化学会大会　合同大会 

脂質ラフトを標的とし多量体化する毒素parasporin-2は細胞膜中で超分子複合体を形成する

第3回パラスポリン研究会 

微生物毒素パラスポリン２のがん細胞破壊作用におけるGPIアンカー型タンパク質の関与

第30回日本分子生物学会年会、第80回日本生化学会大会、合同大会 

Mega assemblage of a mammalian cell-targeting and pore-forming toxin parasporin-2 from Bacillus thuringiensis

40th Annual Meeting of the SIP and 1st IFENSB 

Cell-binding and oligomerization of parasporin-2 are mediated by glycosylphosphatidylinositol -anchored proteins

40th Annual Meeting of the Society for Invertebrate Pathology 

Cytocidal actions of parasporin-2, a novel crystal toxin targeting human cancer cells from Bacillus thuringiensis

IX International Colloquium on Invertebrate Pathology 

Parasporin, an anti-tumor crystal toxin from Bacillus thuringiensis.

GEPROM conferences  

BT菌から発見された新しい結晶性毒素タンパク質「パラスポリン２の細胞認識と毒性」

微研セミナー 

Bacillus thuringiensis から発見された新しい結晶性毒素タンパク質（パラスポリン２）の細胞破壊機構

第52回毒素シンポジウム 

腫瘍結晶性毒素パラスポリン-2はGPIアンカータンパク質を認識し脂質ラフトでオリゴマーを形成する

第５回日本蛋白質科学会年会 

Molecular identification and cytocidal action of parasporin, a protein group of novel crystal toxins targeting to human cells

6TH Annual Pacific Rim Conference on the Biotechnology of Bacillus thuringiensis and its Enviromental Impact 

微生物毒素の生体膜侵入と複合体形成

Grant number：17053019  2005.04 - 2006.03   特定領域研究

微生物毒素による選択的細胞認識の分子機構

Grant number：16770077  2004.04 - 2006.03   若手研究(B)

ミトコンドリアからの情報発信(ミトコンドリアストレスに対する細胞応答)

Grant number：14658233  2002.04 - 2004.03   萌芽研究・萌芽的研究

構造生物学に基づいたミトコンドリア標的化シグナル認識の分子メカニズム

Grant number：12780455  2000.04 - 2002.03   奨励研究(A)

ミトコンドリアプロセシングペプチダ-ゼαサブユニットの機能と基質認識機構

Grant number：09780577  1997.04 - 1999.03   奨励研究(A)