Faculty Profiles - YASUDA Takashi

Papers - YASUDA Takashi

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Two-layered Microwell-array Device for Preparation of Single-neuron Culture Samples Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Journal of Micromechanics and Microengineering ( IOP Publishing ) 34 085011 2024.06

Back-to-back Layered Co-culture on a SiN Porous Membrane for Stable Measurement of Neuronal Extracellular Potentials Reviewed

Satoshi Yoshida, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines ( The Institute of Electrical Engineers of Japan ) 141 ( 7 ) 215 - 221 2021.07

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)

We developed a novel technique for measuring neuronal extracellular potentials in a neuron-astrocyte co-culture system using a SiN (silicon nitride) membrane having microelectrodes and microholes. An 8×8 microelectrode array was formed on one side of a 1 µm-thick SiN membrane, and multiple microholes of 5 µm in diameter were opened through the membrane. Then, neurons were cultured on the microelectrodes after astrocytes were cultured on the back side of the membrane. The neuronal spikes measured in this novel back-to-back co-culture method were compared with those in the conventional method in which both of neurons and astrocytes were co-cultured on the microelectrodes. In the conventional method, formation and enlargement of cellular aggregates made neurons out of touch with microelectrodes, and posed measurement of a small number of spikes. On the other hand, our novel method permitted the firm contact between neurons and microelectrodes and the cell-to-cell interaction through microholes, and therefore neuronal spikes increased drastically with culture time, which led to stable measurement of neuronal extracellular potentials.

DOI： 10.1541/ieejsmas.141.215

Scopus

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CiNii Research

Other Link： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85109212639&origin=inward

Preparation of Single Neuron Samples Using a Two-layered Microwell-array Device Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Proceedings of the 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2022) 456 - 457 2022.10

Astrocyte Potential Measurement Using a Microelectrode Array with Back-to-back Layered Co-culture Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences 576 - 577 2022.10

Capture and Release of Single Cells Using Microholes for High Throughput Analysis of Single Neurons Reviewed International journal

Shuntaro Iwai, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 263 - 264 2021.10

Microelectrode Array with Back-to-back Layered Co-culture of Neurons and Astrocytes Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 707 - 708 2021.10

Co-culture Device for Single Neuron Analysis Using a Microporous SiN Membrane Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 587 - 588 2021.10

Back-to-back co-culture of neurons/astrocytes on a microporous sin membrane and multichannel measurement of neuronal potential using a microelectrode array Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 783 - 784 2019.10

Evaluation of neuronal activity in a neuron-astrocyte co-culture system using a microporous sin membrane Reviewed International journal

Ayaka Nakama, Takashi Yasuda

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 304 - 305 2019.10

Production and Separation of Micrometer-size Extracellular Vesicles Using Microhole Arrays Reviewed

Kazuho Sakurada, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 139 ( 7 ) 195 - 200 2019.07

Particle Patterning Based on Positive Dielectrophoresis Using a Scanning Microelectrode Reviewed

Tomoyuki Yasukawa, Takuma Gotoh, Takashi Yasuda, Masato Suzuki, Fumio Mizutani

31 ( 1 ) 23 - 32 2019.01

Production and separation of micrometer-size vesicles from human lymphocytes using microhole arrays Reviewed International journal

Kazuho Sakurada, Takashi Yasuda

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 3 1421 - 1422 2018.11

Long-term Culture of a Single Neuron using a Microwell with a Porous SiN Membrane Reviewed

Zenmyo Yusuke, Morisako Isamu, Yasuda Takashi

IEEJ Transactions on Sensors and Micromachines 138 ( 7 ) 327 - 328 2018.07

Chemical Stimulation of a Cellular Tissue through a Microhole Array and Multichannel Potential Measurement Using a Microelectrode Array "jointly worked" Reviewed

Ryunosuke Omori, Isamu Morisako, Itsuki Kageyama, Kiyohisa Natsume, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 137 ( 2 ) 59 - 64 2017.02

Real-time Observation and Stimulation of a Single Motile Cell Using High-speed Microrobotic Platform "jointly worked" Reviewed

Belal Ahmad, Tomohiro Kawahara, Takashi Yasuda, Fumihito Arai

2016 International Symposium on Micro-NanoMechatronics and Human Science 2016.11

Specific Metallization of Double-Stranded DNA Using Reducing Group-Labeled Intercalator "jointly worked" Reviewed

Takahiro Himuro, Ryo Araki, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 136 ( 10 ) 425 - 431 2016.10

Formation and Electrical Evaluation of a Single Metallized DNA Nanowire in a Nanochannel "jointly worked" Reviewed

Takahiro Himuro, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Electroanalysis 28 ( 7 ) 1448 - 1454 2016.07

Detection of Deoxyribonuclease Using Site-specifically Metallized DNA "jointly worked" Reviewed

Takahiro Himuro, Tairi Murakami, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015) 1652 - 1654 2015.10

Automatic Droplet Transportation on a Plastic Microfluidic Device Having Wettability Gradient Surface Reviewed

Yuta Nakashima, Yoshitaka Nakanishi, Takashi Yasuda

Review of Scientific Instruments 86 015001 2015.01

Extracellular Potential Measuring Device with a Silicon Nitride Diaphragm Having a Multielectrode Array and Microholes Reviewed

Takashi Yasuda, Kyohei Yonekawa, Ryunosuke Omori, Itsuki Kageyama, Kiyohisa Natsume

Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2014) 983 - 985 2014.10

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Papers - YASUDA Takashi