Faculty Profiles - YASUDA Takashi

Papers - YASUDA Takashi

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Two-layered Microwell-array Device for Preparation of Single-neuron Culture Samples Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Journal of Micromechanics and Microengineering ( IOP Publishing ) 34 085011 2024.06

Back-to-back Layered Co-culture on a SiN Porous Membrane for Stable Measurement of Neuronal Extracellular Potentials Reviewed

Satoshi Yoshida, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines ( The Institute of Electrical Engineers of Japan ) 141 ( 7 ) 215 - 221 2021.07

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)

We developed a novel technique for measuring neuronal extracellular potentials in a neuron-astrocyte co-culture system using a SiN (silicon nitride) membrane having microelectrodes and microholes. An 8×8 microelectrode array was formed on one side of a 1 µm-thick SiN membrane, and multiple microholes of 5 µm in diameter were opened through the membrane. Then, neurons were cultured on the microelectrodes after astrocytes were cultured on the back side of the membrane. The neuronal spikes measured in this novel back-to-back co-culture method were compared with those in the conventional method in which both of neurons and astrocytes were co-cultured on the microelectrodes. In the conventional method, formation and enlargement of cellular aggregates made neurons out of touch with microelectrodes, and posed measurement of a small number of spikes. On the other hand, our novel method permitted the firm contact between neurons and microelectrodes and the cell-to-cell interaction through microholes, and therefore neuronal spikes increased drastically with culture time, which led to stable measurement of neuronal extracellular potentials.

DOI： 10.1541/ieejsmas.141.215

Scopus

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Other Link： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85109212639&origin=inward

Preparation of Single Neuron Samples Using a Two-layered Microwell-array Device Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Proceedings of the 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2022) 456 - 457 2022.10

Astrocyte Potential Measurement Using a Microelectrode Array with Back-to-back Layered Co-culture Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences 576 - 577 2022.10

Capture and Release of Single Cells Using Microholes for High Throughput Analysis of Single Neurons Reviewed International journal

Shuntaro Iwai, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 263 - 264 2021.10

Microelectrode Array with Back-to-back Layered Co-culture of Neurons and Astrocytes Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 707 - 708 2021.10

Co-culture Device for Single Neuron Analysis Using a Microporous SiN Membrane Reviewed International journal

Ayaka Nakama, Takashi Yasuda

Proceedings of the 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2021) 587 - 588 2021.10

Back-to-back co-culture of neurons/astrocytes on a microporous sin membrane and multichannel measurement of neuronal potential using a microelectrode array Reviewed International journal

Satoshi Yoshida, Takashi Yasuda

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 783 - 784 2019.10

Evaluation of neuronal activity in a neuron-astrocyte co-culture system using a microporous sin membrane Reviewed International journal

Ayaka Nakama, Takashi Yasuda

23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 304 - 305 2019.10

Production and Separation of Micrometer-size Extracellular Vesicles Using Microhole Arrays Reviewed

Kazuho Sakurada, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 139 ( 7 ) 195 - 200 2019.07

Particle Patterning Based on Positive Dielectrophoresis Using a Scanning Microelectrode Reviewed

Tomoyuki Yasukawa, Takuma Gotoh, Takashi Yasuda, Masato Suzuki, Fumio Mizutani

31 ( 1 ) 23 - 32 2019.01

Production and separation of micrometer-size vesicles from human lymphocytes using microhole arrays Reviewed International journal

Kazuho Sakurada, Takashi Yasuda

22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 3 1421 - 1422 2018.11

Long-term Culture of a Single Neuron using a Microwell with a Porous SiN Membrane Reviewed

Zenmyo Yusuke, Morisako Isamu, Yasuda Takashi

IEEJ Transactions on Sensors and Micromachines 138 ( 7 ) 327 - 328 2018.07

Chemical Stimulation of a Cellular Tissue through a Microhole Array and Multichannel Potential Measurement Using a Microelectrode Array "jointly worked" Reviewed

Ryunosuke Omori, Isamu Morisako, Itsuki Kageyama, Kiyohisa Natsume, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 137 ( 2 ) 59 - 64 2017.02

Real-time Observation and Stimulation of a Single Motile Cell Using High-speed Microrobotic Platform "jointly worked" Reviewed

Belal Ahmad, Tomohiro Kawahara, Takashi Yasuda, Fumihito Arai

2016 International Symposium on Micro-NanoMechatronics and Human Science 2016.11

Specific Metallization of Double-Stranded DNA Using Reducing Group-Labeled Intercalator "jointly worked" Reviewed

Takahiro Himuro, Ryo Araki, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 136 ( 10 ) 425 - 431 2016.10

Formation and Electrical Evaluation of a Single Metallized DNA Nanowire in a Nanochannel "jointly worked" Reviewed

Takahiro Himuro, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Electroanalysis 28 ( 7 ) 1448 - 1454 2016.07

Detection of Deoxyribonuclease Using Site-specifically Metallized DNA "jointly worked" Reviewed

Takahiro Himuro, Tairi Murakami, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2015) 1652 - 1654 2015.10

Automatic Droplet Transportation on a Plastic Microfluidic Device Having Wettability Gradient Surface Reviewed

Yuta Nakashima, Yoshitaka Nakanishi, Takashi Yasuda

Review of Scientific Instruments 86 015001 2015.01

Extracellular Potential Measuring Device with a Silicon Nitride Diaphragm Having a Multielectrode Array and Microholes Reviewed

Takashi Yasuda, Kyohei Yonekawa, Ryunosuke Omori, Itsuki Kageyama, Kiyohisa Natsume

Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2014) 983 - 985 2014.10

Microrobotic Platform for Mechanical Stimulation of Swimming Microorganism on a Chip Reviewed

Belal Ahmad, Tomohiro Kawahara, Takashi Yasuda, Fumihito Arai

2014 IEEE/RSJ International Conference on Intelligent Rotots and Systems (IROS 2014) 4680 - 4685 2014.09

QCM Detection of GPCR-ligand Binding Using Cell-derived Liposomes "jointly worked" Reviewed

Makoto Yamanaka, Shinji Sueda, Takashi Yasuda

Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2013) 398 - 400 2013.10

Development of a Blood Testing Device Based on Localized Surface Plasmon Resonance "jointly worked" Reviewed

Hiroki Kanamori, Fumiya Takada, Yuhei Sasaki, Makoto Yamanaka, Takashi Yasuda

Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2013) 542 - 544 2013.10

Nanowire Formation Using Specific Metallization of Double-Stranded DNA "jointly worked" Reviewed

Takahiro Himuro, Ryo Araki, Hideyuki Ikedo, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Proceedings of the 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2013) 27 - 31 2013.10

QCM Sensor Using Membrane Proteins of Cell-derived Liposomes "jointly worked" Invited Reviewed

Makoto Yamanaka, Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 133 ( 5 ) 155 - 156 2013.05

Parallel Discrete Chemical Stimulations of Matrix-arrayed Neurospheres Using a Microhole Array Device Reviewed

Takashi Yasuda, Go Takase, Kwang Young Jung, Makoto Yamanaka, Tomoko Tamura, Kanji Yahiro

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012) 977 - 979 2012.10

Generation of a Microliquid Concentration Series Using Wettability Gradient and Electrowetting Reviewed

Takashi Yasuda, Jin Nakamura, Kenichi Nakayama, Makoto Yamanaka

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012) 1456 - 1458 2012.10

QCM Detection of Membrane Protein-ligand Interactions Using Cell-derived Liposomes Reviewed

Makoto Yamanaka, Takashi Yasuda

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012) 380 - 382 2012.10

Formation of a Single Metallized DNA Nanowire in a Nanochannel Reviewed

Takahiro Himuro, Hideyuki Ikedo, Shinobu Sato, Shigeori Takenaka, Takashi Yasuda

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2012) 1879 - 1881 2012.10

Nanowire Formation Using Metallization of Extended and Immobilized DNA, "jointly worked" Reviewed

Takahiro Himuro, Hideyuki Ikedo, Keiichi Ohtsuka, Shigeori Takenaka, Takashi Yasuda

Proceedings of the 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2011) 2011.10

Bidirectional Droplet Transportation Using EWOD-induced Wettability Gradient Reviewed

Takashi Yasuda,Keita Imamura

Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010) 393 - 395 2010.10

Microhole Device for Derivation and Separation of Liposomes from Human Lymphocytes with Synchronized Culture Reviewed

Makoto Yamanaka,Takashi Yasuda

Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010) 1562 - 1564 2010.10

Induction of Cell Size Vesicles from Human Lymphoma Cell Lines and their Application to Drug Carriers Reviewed

Makoto Yamanaka,Shigeki Nakamura,Aiko Inoue,Takashi Yasuda,Yuichi Inoue,Hiroharu Kawahara

Cytotechnology 62 ( 4 ) 287 - 291 2010.04

Blood Plasma Separation and Extraction from a Minute Amount of Blood Using Dielectrophoretic and Capillary Forces Reviewed

Yuta Nakashima,Sakiko Hata,Takashi Yasuda

Sensors and Actuators B: Chemical 145 561 - 569 2010.03

Droplet Transportation on a Wettability Gradient Surface Generated by Electrowetting-on-Dielectric Reviewed

Keita Imamura,Takashi Yasuda

IEEJ Transactions on Sensors and Micromachines 130 ( 1 ) 1 - 5 2010.01

Parallel Preparation of Microliquid Mixtures Using Wettability Gradient and Electrowetting Reviewed

Yuta Nakashima,Takashi Yasuda

Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) 725 - 727 2009.11

Liposome Derivation from Human Lymphocytes in a Microchannel Reviewed

Makoto Yamanaka,Hiroharu Kawahara,Takashi Yasuda

Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) 1213 - 1215 2009.11

Electrochemical Detection of Deoxyribonuclease in Microliquid Reviewed

Yoshikazu Sakata,Shotaro Hirokawa,Shinobu Sato,Shigeori Takenaka,Takashi Yasuda

Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) 305 - 307 2009.11

Chemical Stimulation of Neurospheres through Microholes Opened in Microwell Bottoms Reviewed

Yuta Nakashima,Naoki Mimaru,Tatsuhiko Tsujihashi,Tomoko Tamura,Kanji Yahiro,Takashi Yasuda

Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2009) 1174 - 1176 2009.11

Droplet Transportation Using EWOD-Induced Wettability Gradient Reviewed

Takashi Yasuda,Keita Imamura,Keisuke Hirase

Proceedings of the 15th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers 2009) 413 - 416 2009.06

Immobilization of a Ferrocenyloligonucleotide on the Electrode and its Application to DNase I Assay Reviewed

Shigeori Takenaka,Shinobu Sato,Masanori Kanazawa,Katsuya Fujita,Shota Ohtsuka,Keiichi Ohtsuka,Shotaro Hirokawa,Yoshikazu Sakata,Takashi Yasuda

The IUMRS International Conference in Asia 2008 (IUMRS-ICA 2008) 2008.12

Characterization of Cell-sized Vesicles Induced from Human Lymphoid Cell Lines Reviewed

Makoto Yamanaka,Shigeki Nakamura,Aiko Inoue,Takashi Yasuda,Yuichi Inoue,Hiroharu Kawahara

The 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2008) 2008.11

Microfluidic Device for Blood Plasma Extraction Using Dielectrophoretic Blood Cell Removal Reviewed

Yuta Nakashima,Takashi Yasuda

Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 1 805 - 807 2008.10

Tubeless Microfluidic Sampling/Mixing Device Using Wettability Gradient and Electrowetting Reviewed

Takashi Yasuda,Shouhei Harada

Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2 1396 - 1398 2008.10

A Superhydrophobic Microvalve for Manipulating Microliquids Containing Biological Molecules Reviewed

Takashi Yasuda,Katsutoshi Ishizuka,Mika Ezoe

IEEJ Transactions on Electrical and Electronic Engineering 3 290 - 296 2008.04

Microfluidic Dispensing Device Using Wettability Gradient and Electrowetting Reviewed

Takashi Yasuda,Shouhei Harada,Katsuya Daimon

IEEJ Transactions on Sensors and Micromachines 128 ( 3 ) 75 - 79 2008.03

Pressure-driven Microvalve Array for Controlling Chemical Release to Cultured Cells Reviewed

Takashi Yasuda,Tetsunari Yamami,Hidetsugu Yano

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007) 1 754 - 756 2007.10

Blood Plasma Extraction from a Minute Amount of Blood Using Dielectrophoresis Reviewed

Yuta Nakashima,Takashi Yasuda

Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2007) 1 706 - 708 2007.10

Cell Differentiation Guidance Using Chemical Stimulation Controlled by a Microfluidic Device Reviewed

Yuta Nakashima,Takashi Yasuda

Sensors and Actuators A 139 252 - 258 2007.04

Hepatocyte Spheroid Culture on a Polydimethylsiloxane Chip Having Microcavities Reviewed

Kohji Nakazawa,Yumiko Izumi,Junji Fukuda,Takashi Yasuda

Journal of Biomaterials Science, Polymer Edition 17 ( 8 ) 859 - 873 2006.04

Neurotransmitter Sensing Device with Molecule-Permeable Platinum Filled in Nano-holes Reviewed

Takashi Yasuda,Kazuyuki Shinohara,Tetsunori Hirata

10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2006) 1 747 - 749 2006.04

Cell Growth Control Using Chemical Stimulation Regulated by Microvalve Switching Reviewed

Yuta Nakashima,Takashi Yasuda

10th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS2006) 1 440 - 442 2006.04

Controllable Chemical Stimulation for Cell Differentiation Guidance Using a Microfluidic Device Reviewed

Yuta Nakashima,Takashi Yasuda

Asia-Pacific Conference of Transducers and Micro-Nano Technology (APCOT 2006) BMFS-A0504 (CD-ROM) 2006.04

Fabrication of a Microfluidic Device for Axonal Guidance Reviewed

Yuta Nakashima,Takashi Yasuda

Journal of Robotics and Mechatronics 17 ( 2 ) 158 - 163 2005.04

Microfluidic Device for Axonal Elongation Control Reviewed

Yuta Nakashima,Takashi Yasuda

Technical digest of the 13th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '05) 2 1741 - 1744 2005.04

Super-Hydrophobic Passive Microvalve for Manipulating Protein-Containing Liquids Reviewed

Takashi Yasuda,Mika Ezoe,Katsutoshi Ishizuka

Proceedings of the Ninth International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2005) 2 1102 - 1104 2005.04

6脚ロボットの方向転換歩容生成ネットワーク Reviewed

岩瀬英治,加藤慎一朗,安田隆,下山勲

日本ロボット学会誌 22 ( 4 ) 492 - 498 2004.04

Automatic Transportation of a Droplet on a Wettability Gradient Surface Reviewed

Takashi Yasuda,Koichi Suzuki,Isao Shimoyama

Proceedings of Seventh International Conference on Micro Total Analysis Systems (microTAS 2003) 1129 - 1132 2003.10

Fabrication of a Microfluidic Device for Axonal Growth Control Reviewed

Takashi Yasuda,Tomoyuki Higashi,Yuta Nakashima

Proceedings of the 12th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers '03) 2 1259 - 1262 2003.04

マイクロ熱電対プローブを用いた生体温度計測 Reviewed

安田隆,鈴鹿菜穂,栗原一貴,下山勲

電気学会論文誌 122-E ( 12 ) 567 - 572 2002.12

柔軟素材に埋め込み可能なマイクロプレーナコイル触覚センサ Reviewed

二井信行,安田隆,下山勲

電気学会論文誌 121-E ( 9 ) 513 - 518 2001.09

MEMS Meets Insects Reviewed

Isao Shimoyama,Kazunori Hoshino,Yoshihiko Ozaki,Satoshi Tamaki,Shoji Takeuchi,Takashi Yasuda

11th International Conference on Solid-State Sensors and Actuators (Transducers '01/Eurosensors XV) 1 6 - 9 2001.06

Micromachined Planar Coil Tactile Sensor Implantable in Soft Structure Reviewed

Nobuyuki Futai,Takashi Yasuda,Masayuki Inaba,Isao Shimoyama,Hirochika Inoue

ASME International Mechanical Engineering Congress and Exposition MEMS-Vol.2 51 - 56 2000.11

Walking Microrobot Mechanism with an Exoskeleton Reviewed

Tetsuji Dohi,Kohsuke Kishi,Takashi Yasuda,Isao Shimoyama

Journal of Robotics and Mechatronics 12 ( 2 ) 150 - 157 2000.04

Microrobot Actuated by a Vibration Energy Field Reviewed

Sensors & Actuators : A. Physical 43 ( 1-3 ) 366 - 370 1994.04

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Papers - YASUDA Takashi