加藤 珠樹 (カトウ タマキ)

KATO Tamaki

写真a

職名

准教授

研究室住所

福岡県北九州市若松区ひびきの2-4

研究分野・キーワード

生物有機科学、ペプチド、コンフォメーション

ホームページ

http://www.life.kyutech.ac.jp/~tmkato/

出身大学 【 表示 / 非表示

  • 1986年03月   九州大学   理学部   化学   卒業   日本国

出身大学院 【 表示 / 非表示

  • 1991年03月  九州大学  理学研究科  化学  博士課程・博士後期課程  中退  日本国

取得学位 【 表示 / 非表示

  • 九州大学 -  博士(理学)  1994年09月

学内職務経歴 【 表示 / 非表示

  • 2014年04月
    -
    継続中

    九州工業大学   大学院生命体工学研究科   生体機能応用工学専攻   准教授  

学外略歴 【 表示 / 非表示

  • 1996年04月
    -
    1998年03月

    カナダ国立研究機構バイオテクノロジー研究所   博士研究員   カナダ

  • 1991年04月
    -
    1995年03月

    東京農工大学工学部物質生物工学科   助手   日本国

専門分野(科研費分類) 【 表示 / 非表示

  • 生物分子化学

  • 生物分子化学

 

論文 【 表示 / 非表示

  • Dual emissive bispyrene peptide probes for highly sensitive measurements of trypsin activity and evaluation of trypsin inhibitors

    Sato D., Kondo T., Kato T.

    Bioorganic and Medicinal Chemistry    26 ( 12 ) 3468 - 3473   2018年07月  [査読有り]

     概要を見る

    © 2018 Elsevier Ltd Peptide substrates were double labeled with pyrenes to prepare fluorescent probes for highly sensitive detection of protease activity and evaluation of protease inhibitors using pyrene monomer/excimer signals. Two proximate pyrene moieties formed excited state dimers in the probes, and these pyrene excimer formations were dissociated by tryptic digestion. The specificity constant of the optimum bispyrene peptide probe was 2.7 times higher than that of the conventional peptide-4-methylcoumarin amide. Moreover, our probe had high sensitivity with an estimated detection limit for trypsin of 4.11 pM. The half maximal inhibitory concentration and dissociation constant of the Bowman–Birk inhibitor were successfully estimated.

    DOI Scopus

  • Photophysical Characterization and BSA Interaction of Direct Ring Carboxy Functionalized Symmetrical squaraine Dyes

    Saikiran M., Pandey S., Hayase S., Kato T.

    Journal of Physics: Conference Series    924 ( 1 )   2017年12月  [査読有り]

     概要を見る

    © Published under licence by IOP Publishing Ltd. A series of far-red sensitive symmetrical squaraine dyes bearing direct-COOH functionalized indole ring were synthesized, characterized and subjected to photophysical investigations. These symmetrical squaraine dyes were then subjected to investigate their interaction with bovine serum albumin (BSA) in Phosphate buffer solutions. All the squaraine dyes under investigation exhibit intense and sharp optical absorption mainly in the far-red wavelength region from 550 nm-700 nm having very high molar extinction coefficients from 1.3 × 10 5 dm 3 .mol -1 .cm -1 . A very small Stokes shift of 10-17 nm indicates the rigid conformational structure of squaraine chromophore. Interaction of these dyes with BSA leads to not only enhanced emission intensity but also bathochromically shifted absorption maximum due to formation of dye-BSA conjugate. These dyes bind strongly with BSA having about an order of magnitude higher binding constant as compared to the reported squaraine dyes. Amongst the symmetrical squaraine dyes investigated in this work one bearing substituents like trifluorobutyl as alkyl chain at N-position of indole ring and carboxylic acid on benzene ring at the terminal (SQ-26) exhibited highest association with the BSA having very high binding constant 8.01 × 10 6 M -1 .

    DOI Scopus

  • Efficient near infrared fluorescence detection of elastase enzyme using peptide-bound unsymmetrical squaraine dye

    Saikiran M., Sato D., Pandey S., Hayase S., Kato T.

    Bioorganic and Medicinal Chemistry Letters    27   4024 - 4029   2017年06月  [査読有り]

    DOI

  • Photophysical characterization and BSA interaction of the direct ring carboxy functionalized unsymmetrical NIR cyanine dyes

    Saikiran M., Sato D., Pandey S., Ohta T., Hayase S., Kato T.

    Dyes and Pigments    140   6 - 13   2017年01月  [査読有り]

    DOI

  • Novel fluorescent substrates for detection of trypsin activity and inhibitor screening by self-quenching

    Sato D., Kato T.

    Bioorganic and Medicinal Chemistry Letters    26 ( 23 ) 5736 - 5740   2016年12月  [査読有り]

     概要を見る

    © 2016 Elsevier Ltd A group of self-quenching-based substrates with two fluorescent peptides for detection of trypsin activity was designed and synthesized. The substrates could be easily synthesized using simple solid-phase peptide synthesis techniques. Two fluorescent peptide substrates for trypsin were conjugated to the amino groups of lysine as a branched unit. The fluorescence of these substrates was self-quenched owing to the highly assembled fluorophores on the substrates. The release of these concentrated fluorophores by proteases allows for fluorescence recovery. Self-quenching reduced the fluorescence of the substrates by 64.1%, and the fluorescence intensity was recovered by the release of the fluorophores from the substrate peptides via tryptic cleavage. The kinetic assay revealed that the k cat /K m values of the substrates were almost comparable to those of the standard fluorescent probe, peptide-MCA. The detection limit for trypsin was 111 pM, and the calculation of IC 50 and K i values for the Bowman–Birk inhibitor was achieved using these substrates. These easily synthesizable self-quenching-based substrates have the potential to be useful for the detection of other disease-related protease activities.

    DOI Scopus

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口頭発表・ポスター発表等 【 表示 / 非表示

  • 固定化した蛍光性ペプチドによる酵素活性の検出

    山本祥太郎 物質工学科応用化学コース

    日本化学会第91春季年会(2011)  (日本 横浜市)  2011年03月  -  2011年03月   

  • 蛍光性ペプチドとリシンデンドリマーを用いた酵素活性の検出

    服部司 生命体工学研究科生体機能専攻

    日本化学会第91春季年会(2011)  (日本 横浜市)  2011年03月  -  2011年03月   

  • 環状ペプチドナノチューブ形成の解析

    武哲 生命体工学研究科生体機能専攻

    日本化学会第91春季年会(2011)  (日本 横浜市)  2011年03月  -  2011年03月   

  • Synthesis and characterization of antimicrobial peptide surfactant with dendritic lysine groups

    Junko Kuwahara 福岡工業大学

    5th International Peptide Symposium  (日本 京都)  2010年12月  -  2010年12月   

  • Self-assembling Cyclic Hexapeptides for Peptide Nanotube Formation

    Asuka Kohchi 生命体工学研究科生体機能専攻

    5th International Peptide Symposium  (日本 京都)  2010年12月  -  2010年12月   

    CiNii

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担当授業科目 【 表示 / 非表示

  • 2019年度  生物機能構造

  • 2019年度  バイオインフォマティクス演習

  • 2019年度  生物有機化学

  • 2019年度  化学Ⅰ

  • 2018年度  生物機能構造

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