IKENO Shinya

写真a

Title

Associate Professor

Laboratory

2-4 Hibikino, Wakamatsu-ku, Kitakyushu-shi, Fukuoka

Research Fields, Keywords

Bioprocess, Functional peptide, Nanoparticles

Degree 【 display / non-display

  • Kanazawa University -  Doctor of Engineering  2003.03

Biography in Kyutech 【 display / non-display

  • 2014.04
    -
    Now

    Kyushu Institute of TechnologyGraduate School of Life Science and Systems Engineering   Department of Biological Functions Engineering   Associate Professor  

Specialized Field (scientific research fund) 【 display / non-display

  • Biofunction/Bioprocess

 

Publications (Article) 【 display / non-display

  • A short peptide designed from late-embryogenesis abundant protein enhances acid tolerance in Escherichia coli

    Khaled Metwally, Shinya Ikeno

    Applied Biochemistry and Biotechnology      2020.02  [Refereed]

    DOI

  • Interaction study of peptide-PAMAM as potential bio-nanogate for detecting anti-hepatitis B surface antigen

    ( Elsevier )  185   2019.11  [Refereed]

    DOI

  • Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein

      503 ( 2 ) 910 - 914   2018.09  [Refereed]

     View Summary

    © 2018 Elsevier Inc. Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Recombinant Escherichia coli BL21 (DE3) expressing functional LEA short peptide in vivo were exposed to UVA and UVC radiation for 4, 6, and 8 h. E. coli transformants expressing the LEA peptide showed higher cell viability under both UVA and UVC treatment at all time points as compared with that of the control. Furthermore, the cells expressing LEA peptide showed a higher number of colony-forming units per dilution under UVA and UVC treatment. These results suggested that expression of the short peptide could be useful for the development of genetically modified organisms and in applications that require resilience of organisms to UV radiation.

    DOI Scopus

  • In vivo expression of a short peptide designed from late embryogenesis abundant protein for enhancing abiotic stress tolerance in Escherichia coli

      492 ( 3 ) 386 - 390   2017.10  [Refereed]

     View Summary

    © 2017 Elsevier Inc. In vivo functional analyses of a late embryogenesis abundant (LEA) short peptide expressed in recombinant Escherichia coli BL21 (DE3) were carried out under abiotic stress (salt, heat, and cold) conditions. Our LEA peptide was derived from the Polypedilum vanderplanki group 3 LEA protein based on distinctive conserved amino acid motif sequences. We focused on high-salt (5% and 7% NaCl) concentrations to evaluate the functional relevance of the peptide under abiotic salt stress. E. coli transformants expressing the LEA peptide showed higher cell viability than the control not expressing the peptide when transferred to a medium containing 5% and 7% NaCl; cells expressing LEA peptide showed a higher number of colony-forming units per dilution under the high salt stress condition. Moreover, expression of the LEA peptide resulted in greater cell survival under heat (48 °C) and cold (4 °C) stress. These results suggest that LEA short peptide co-expression could be useful for developing genetically modified organisms and in applications to prevent E. coli cell death under high salt, heat, and cold stress.

    DOI Scopus

  • Construction and characterization of mutated LEA peptides in Escherichia coli to develop an efficient protein expression system

        2017.08  [Refereed]

    DOI

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Conference Prsentations (Oral, Poster) 【 display / non-display

  • Characterization and optimization of garbage enzyme from sources of organic waste and its potential industrial application

    Nurul Ain Syukriyah Ahmad Muhamud, Rashidah Abdul Rahim, Shinya Ikeno

    12th Japan-Korea joint symposium on Bio-microsensing Technology  2019.12  -  2019.12 

  • High expression of Silicatein by using LEA peptide co-expression system

    Kouki Sato, Kazunori Nakashima, Shinya Ikeno

    12th Japan-Korea joint symposium on Bio-microsensing Technology  2019.12  -  2019.12 

  • Codon usage of LEA peptide is affect to efficient expression of GFP in Escherichia coli

    Junpei Shoji, Khaled Metwally, Shinya Ikeno

    12th Japan-Korea joint symposium on Bio-microsensing Technology  2019.12  -  2019.12 

  • Development of a peptide-integrated microfluidic system for the electrochemical detection of Newcastle disease virus

    Abd Muain, Amir Syahir Amir Hamzah, Lim Hong Ngee, Shinya Ikeno, Asilah Ahmad Tajudin

    12th Japan-Korea joint symposium on Bio-microsensing Technology  2019.12  -  2019.12 

  • Responses of Deinococcus radiodurans R1 upon exposure to ultraviolet (UV) radiation

    Khairunisa Amira Ahmad, Asilah Ahmad Tajudin, Shinya Ikeno and Amir Syahir Amir Hamzah

    12th Japan-Korea joint symposium on Bio-microsensing Technology  2019.12  -  2019.12 

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Lectures 【 display / non-display

  • Development of functional short peptide and its application

    Pure and Applied Chemistry International Conference 2020   2020.02.14 

  • Functional peptide: its full potential and application

    International Congress of The Malaysian Society For Microbiology 2019   2019.11.13 

  • c-face配向性を有するZnO球状粒子の微生物殺菌への応用

    平成24年度第1回 食品・バイオテクノロジー技術研究会講演会 ( リファレンス駅東ビル 3階会議室 )  2012.10.03  産業技術研究所 計測・診断システム研究協議会

  • バイオサーベイランスを目的とした人工酵素の分子設計・合成とそのセンサ応用

    金沢大学 第10回バイオサイエンスシンポジウム ( 金沢 )  2007.11.09