研究者詳細 - 佐藤　しのぶ

論文 - 佐藤　しのぶ

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Cyclic anthraquinone derivatives, unique G-quadruplex binders, selectively induce cancer cell apoptosis and inhibit tumor growth 査読有り国際誌

Fukuda H., Zou T., Fujii S., Sato S., Wakahara D., Higashi S., Tseng T.Y., Chang T.C., Yada N., Matsuo K., Habu M., Tominaga K., Takeuchi H., Takenaka S.

PNAS Nexus 2 ( 7 ) 2023年07月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Cyclic anthraquinone derivatives (cAQs), which link two side chains of 1, 5-disubstituted anthraquinone as a threading DNA intercalator, have been developed as G-quartet (G4) DNA-specific ligands. Among the cAQs, cAQ-mBen linked through the 1, 3-position of benzene had the strongest affinity for G4 recognition and stabilization in vitro and was confirmed to bind to the G4 structure in vivo, selectively inhibiting cancer cell proliferation in correlation with telomerase expression levels and triggering cell apoptosis. RNA-sequencing analysis further indicated that differentially expressed genes regulated by cAQ-mBen were profiled with more potential quadruplex-forming sequences. In the treatment of the tumor-bearing mouse model, cAQ-mBen could effectively reduce tumor tissue and had less adverse effects on healthy tissue. These results suggest that cAQ-mBen can be a potential cancer therapeutic agent as a G4 binder.

DOI： 10.1093/pnasnexus/pgad211

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Naphthalene Diimides Carrying Two β-Cyclodextrins Prefer Telomere RNA G-Quadruplex Recognition 査読有り国際誌

Zou T., Sato Y., Kaneyoshi S., Mano K., Yasukawa R., Nakano Y., Fujii S., Sato S., Takenaka S.

Molecules 27 ( 13 ) 2022年07月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Newly synthesized naphthalene diimide carrying two β-cyclodextrins (NDI-β-CyDs) showed improved specificity for the parallel G-quadruplex structure alongside the hybrid G-quadruplex structure. Specifically, the highest binding affinity of NDI-β-CyDs for the telomere RNA G-quadruplex was observed. The binding simulation indicated that β-cyclodextrins might be available for loop nucleobase inclusion under its complex.

DOI： 10.3390/molecules27134053

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Cyclic ferrocenylnaphthalene diimides as a probe for electrochemical telomerase assay 査読有り国際誌

Kaneyoshi S., Eguchi N., Fujimoto K., Fujii S., Sato S., Takenaka S.

Journal of Inorganic Biochemistry 230 2022年05月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

Novel cyclic naphthalene diimides, 8 and 12, containing ferrocene in the cyclic linker were synthesized as G-quartet (G4) specific electrochemical ligands via the reaction of 1,1′-ferrocenedipropanoic acid and the terminal amine moieties of naphthalene diimides with varying linker lengths. The redox potentials of 8 and 12 were ca. 0.2 V (vs. Ag/AgCl), and the background current in an electrolyte was successfully suppressed. Both 8 and 12 bound to TA-core, representing human telomere G4, with K = 4.4 and 38 × 105 M−1, respectively. The current response of 12 to an electrode immobilized with G4 was the highest among the acyclic derivatives, suggesting its potential application in electrochemical telomerase assays.

DOI： 10.1016/j.jinorgbio.2022.111746

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Electrochemical telomerase assay with ferrocenylnaphthalene diimide as a tetraplex DNA-specific binder 査読有り国際誌

Sato S., Kondo H., Nojima T., Takenaka S.

Analytical Chemistry 77 ( 22 ) 7304 - 7309 2005年11月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Spectroscopic studies revealed that ferrocenylnaphthalene diimide (1) can bind to tetraplex DNA at high potassium ion concentration. The tetraplex DNA was stabilized by the binding of 1, and this effect was larger than that of any other tetraplex stabilizers, which are known as a telomerase inhibitor. Quantitative analysis with circular dichroism and a quartz crystal microbalance strongly suggested a 3:1 binding stoichiometry of 1 to the tetraplex DNA. The telomere sequence could be extended by telomerase with the telomerase substrate primer on the surface of an electrode as proven by an increased current signal of 1 bound to the tetraplex DNA formed on the electrode. This is the first example of electrochemical detection of telomerase activity without relying on PCR. © 2005 American Chemical Society.

DOI： 10.1021/ac0510235

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Electrochemically active DNA ligands for gene detection: present and future 招待有り査読有り国際誌

Takenaka S., Sato S.

Analytical Sciences 2025年01月

担当区分：筆頭著者記述言語：英語掲載種別：記事・総説・解説・論説等（学術雑誌）

Electrochemical gene sensing methods are gaining attention as diagnostic chips. Here, we review the electrochemically active DNA ligand-based sensing methods. Various DNA ligands have been reported in these studies, among which metal complexes, methylene blue, and ferrocenyl naphthalene diimide (FND) have been studied in detail. DNA probe immobilized electrodes have been created, hybridization reactions on the electrodes with target DNA fragments have been performed, and electrochemical gene detection has been possible using these DNA ligands. An example of the realization of this system is the successful and accurate cancer diagnosis using FND to examine abnormal methylation of the hTERT gene, providing reassurance about the system's reliability. In addition, electrochemical detection of PCR products has been realized using the current decrease due to the double-stranded DNA binding of methylene blue although it is a signal-off system. A naphthalene diimide derivative with ferrocene and β-CD, FNC, increased the current upon double-stranded DNA binding. Using these FNCs, the detection of PCR products in a homogeneous system was realized. Electrochemical qPCR was realized with these ligands. Since FNDs also bind strongly to tetraplex or G-quadruplex (G4) DNA, we succeeded in electrochemically detecting telomerase activity, which is known as a cancer marker, using FNDs to detect the amount of telomeric DNA elongation, which is its substrate, as the amount of G4 DNA. This technique has realized compassionate cancer diagnosis from oral swab fluid. It is known that G4 is also present in viral genome RNA, and a viral testing method using G4 is expected to be a potential alternative to PCR. The first example was the electrochemical detection of novel coronaviruses using incFND as an RNA G4 ligand.

DOI： 10.1007/s44211-025-00745-5

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Biotinylated cyclic naphthalene diimide as a searching tool for G4 sites on the genome 査読有り国際誌

Fujii S., Sato S., Hidaka R., Takenaka S.

Analytical Sciences 40 ( 5 ) 943 - 950 2024年05月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

A biotinyl cyclic naphthalene diimide (biotinyl cNDI) (1), in which biotin is introduced on the cyclic linker chain of cNDI with high G-quadruplex (G4) specificity, was synthesized. 1 was used for binding analysis to G4 DNAs such as c-myc, c-kit, CEGF, or TA-core. The results showed that 1 bind to G4 DNAs with high affinity and, especially, two molecules of 1 bind to c-myc DNA from top and bottom of G4 site at K = 3.9 × 10−6 M−1 without changing the G4 structure. As a pulldown assay, 1 and streptavidin magnetic beads could be used to recover a c-myc DNA or 120-mer DNA fragment having single c-myc sequence. The qPCR results for the 120-meric DNAs showed that more than 50% of genomic DNA fragments could be recovered by this pulldown assay. The results obtained here might allow the recovery of G4-containing DNA fragments from genomic DNA to analyze the true G4 present in the genome. Graphic abstract: (Figure presented.)

DOI： 10.1007/s44211-024-00551-5

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Novel analytical technologies contributing to clinical and pharmaceutical research fields 招待有り査読有り国際誌

Takenaka S., Sato S., Hamase K., Hayama T.

Analytical Sciences 40 ( 5 ) 2024年05月

Synthesis of cNDI-Peptide-Dimer as a G4 Cluster-Selective Binder and its Interaction with G4 Dimer 査読有り国際誌

Ono K., Sato S., Takenaka S.

ChemistrySelect 9 ( 16 ) 2024年04月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

The amino acid unit carrying cyclic naphthalene diimide (cNDI) was synthesized by linking the amino moiety of cNDI as a G4-specific ligand with the γ-carboxylic acid moiety of Fmoc-Glu. cNDI-peptide-dimer, 1 was synthesized by peptide synthesizer using this unit. 1 exhibited an extremely strong binding constant of the order of 108 M−1 with telomeric G4 dimer, probably due to cooperative stacking of the its two G4 planes. It also showed an IC50 at the nM level by the TRAP assay. The results obtained here are expected to enable the design of peptide ligands with multiple cNDI sites targeting the G4 cluster by using the amino acid units of cNDI.

DOI： 10.1002/slct.202401062

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Evaluation of the accuracy of hTERT gene aberrant methylation using electrochemical hybridization assay and liquid-based cytology in screening for oral squamous cell carcinoma 査読有り国際誌

Haraguchi K., Sato S., Habu M., Yada N., Hayakawa M., Sasaguri M., Yoshioka I., Tominaga K., Takenaka S.

Electroanalysis 35 ( 8 ) 2023年08月

記述言語：英語掲載種別：研究論文（学術雑誌）

In order to improve the survival rate of oral squamous cell carcinoma (OSCC) patients, a reliable diagnostic method for early OSCC detection is required that is minimally invasive, less burdensome to the patient, and has high sensitivity and specificity. Therefore, we performed the detection of abnormal methylation at three locations in the hTERT promoter region of oral exfoliated cells by employing the ferrocenylnaphthalene diimide (FND)-based electrochemical hybridization assay (FND-EHA) using three types of DNA probe-immobilized electrodes. We also performed liquid cytology using oral exfoliated cells and compared these obtained data to evaluate whether FND- EHA can be used as an OSCC screening system. The results showed a good correlation between this method and conventional OSCC screening, and cytology. In addition, FND-EHA was also able to determine samples that had been ambiguously determined by liquid cytology. This indicates that FND-EHA may be useful as an OSCC screening system.

DOI： 10.1002/elan.202200564

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ウイルス検査としてのPCRの原理と限界招待有り査読有り

佐藤しのぶ

化学と教育 ( 日本化学会 ) 71 ( 1 ) 8 - 11 2023年01月

Fluorescence Imaging of Extracellular Potassium Ion Using Potassium Sensing Oligonucleotide 招待有り査読有り国際誌

Sato S., Ohzawa S., Sota K., Sakamoto N., Udo A., Sueda S., Matsuda T., Nagai T., Takenaka S.

Frontiers in Chemistry 10 2022年07月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Potassium-sensing oligonucleotide, PSO, a conjugate of a quadruplex structure-forming oligonucleotide with a peptide incorporating a Förster Resonance Energy Transfer (FRET) chromophore pair, has been developed for fluorescent detection of potassium ion (K+) in aqueous medium. PSO 1 could be introduced into cells for real-time imaging of cytoplasmic K+ concentrations. To perform fluorescent imaging of K+ on the cell surface, we synthesized twelve PSO derivatives with different types of peptide types and lengths, and oligonucleotide sequences including thrombin-binding aptamer (TBA) sequences with FAM and TAMRA as a FRET chromophore pair, and evaluated their performance. 1 was shown to respond selectively to K+, not to most ions present in vivo, and to show reciprocal fluorescence changes in response to K+ concentration. For the peptide chains and oligonucleotide sequences examined in this study, the PSO derivatives had Kd values for K+ in the range of 5–30 mM. All PSO derivatives showed high K+ selectivity even in the presence of excess Na+. The PSO derivatives were successfully localized to the cell surface by biotinylated concanavalin A (ConA) or sulfo-NHS-biotin via streptavidin (StAv). Fluorescence imaging of extracellular K+ upon addition of apoptosis inducers was successfully achieved by 1 localized to the cell surface.

DOI： 10.3389/fchem.2022.922094

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Replication Control of Human Telomere G-Quadruplex DNA by G-Quadruplex Ligands Dependent on Solution Environment 査読有り国際誌

Takahashi S., Bhowmik S., Sato S., Takenaka S., Sugimoto N.

Life 12 ( 4 ) 2022年04月

記述言語：英語掲載種別：研究論文（学術雑誌）

The human telomere region is known to contain guanine-rich repeats and form a guaninequadruplex (G4) structure. As telomeres play a role in the regulation of cancer progression, ligands that specifically bind and stabilize G4 have potential therapeutic applications. However, as the human telomere sequence can form G4 with various topologies due to direct interaction by ligands and indirect interaction by the solution environment, it is of great interest to study the topologydependent control of replication by ligands. In the present study, a DNA replication assay of a template with a human telomere G4 sequence in the presence of various ligands was performed. Cyclic naphthalene diimides (cNDI1 and cNDI2) efficiently increased the replication stall of the template DNA at G4 with an anti-parallel topology. This inhibition was stability-dependent and topology-selective, as the replication of templates with hybrid or parallel G4 structures was not affected by the cNDI and cNDI2. Moreover, the G4 ligand fisetin repressed replication with selectivity for anti-parallel and hybrid G4 structures without stabilization. Finally, the method used, referred to as quantitative study of topology-dependent replication (QSTR), was adopted to evaluate the correlation between the replication kinetics and the stability of G4. Compared to previous results obtained using a modified human telomere sequence, the relationship between the stability of G4 and the effect on the topology-dependent replication varied. Our results suggest that native human telomere G4 is more flexible than the modified sequence for interacting with ligands. These findings indicate that the modification of the human telomeric sequence forces G4 to rigidly form a specific structure of G4, which can restrict the change in topology-dependent replication by some ligands.

DOI： 10.3390/life12040553

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Electrochemical Method for G-quartet RNA Detection of COVID-19 Based on Cyclic Ferrocenylnaphthalene Diimide 査読有り国際誌

Kaneyoshi S., Murakami S., Ikeda T., Fujimoto K., Fujii S., Sato S., Takenaka S.

Electroanalysis 35 ( 4 ) 2022年01月

担当区分：責任著者記述言語：英語掲載種別：研究論文（学術雑誌）

To develop an electrochemical detection method for COVID-19, we have investigated the interaction between RG-1, a characteristic parallel four-strand RNA component of the COVID-19 genome, and cyclic ferrocenylnaphthalene diimides carrying different linker lengths 1 and 2, which bind to RG-1 more strongly than cyclic naphthalene diimide 3 that lacks a ferrocene moiety. In particular, the binding affinity of 1 having short one showed the highest binding affinity to RG-1, and the redox current of 1 increased in the presence of RG-1 using a glassy carbon electrode. This increasing current of 1 in the presence of c-myc as a parallel G4 DNA or single-stranded RNA was lower than that in the presence of RG-1. Several RG-1 was electrochemically detected at the nanomolar level by 1.

DOI： 10.1002/elan.202200427

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Substituent effects of cyclic naphthalene diimide on G-quadruplex binding and the inhibition of cancer cell growth 査読有り

Fukuda H., Sato S., Zou T., Higashi S., Takahashi O., Habu M., Sasaguri M., Tominaga K., Takenaka S., Takeuchi H.

Bioorganic and Medicinal Chemistry Letters 50 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Interaction of cyclic naphthalene diimide derivatives (cNDIs), 1–4, with TA-core and c-myc as G-quartet (G4) DNA was studied under dilute or molecular crowding condition. Binding study for TA-core based on an isothermal titration calorimetry showed that 1–4 has 106 M−1 order of binding affinity with the following order: 1 > 4 > 2 > 3 under both conditions. Meting temperature (Tm) of TA-core obtained from the temperature dependence of circular dichroism spectra shows that TA-core was most stabilized by 4, which is in agreement with the result of PCR stop assay and the stabilization effect for 1–3 was correlated with their binding affinity under dilute condition. 3 showed specific growth inhibition of cancer cell line Ca9-22 at <0.03 μM of IC50, with no inhibitory effect against normal bone marrow cells. 3, which has highest value of ΔH/ΔG, shows the highest inhibition ability for Ca9-22, carrying a highest expression level of telomerase mRNA.

DOI： 10.1016/j.bmcl.2021.128323

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Chemical Modulation of DNA Replication along G-Quadruplex Based on Topology-Dependent Ligand Binding 査読有り

Takahashi S., Kotar A., Tateishi-Karimata H., Bhowmik S., Wang Z.F., Chang T.C., Sato S., Takenaka S., Plavec J., Sugimoto N.

Journal of the American Chemical Society 143 ( 40 ) 16458 - 16469 2021年10月

記述言語：英語掲載種別：研究論文（学術雑誌）

Ligands that bind to and stabilize guanine-quadruplex (G4) structures to regulate DNA replication have therapeutic potential for cancer and neurodegenerative diseases. Because there are several G4 topologies, ligands that bind to their specific types may have the ability to preferentially regulate the replication of only certain genes. Here, we demonstrated that binding ligands stalled the replication of template DNA at G4, depending on different topologies. For example, naphthalene diimide derivatives bound to the G-quartet of G4 with an additional interaction between the ligand and the loop region of a hybrid G4 type from human telomeres, which efficiently repressed the replication of the G4. Thus, these inhibitory effects were not only stability-dependent but also topology-selective based on the manner in which G4 structures interacted with G4 ligands. Our original method, referred to as a quantitative study of topology-dependent replication (QSTR), was developed to evaluate correlations between replication rate and G4 stability. QSTR enabled the systematic categorization of ligands based on topology-dependent binding. It also demonstrated accuracy in determining quantitatively how G4 ligands control the intermediate state of replication and the kinetics of G4 unwinding. Hence, the QSTR index would facilitate the design of new drugs capable of controlling the topology-dependent regulation of gene expression.

DOI： 10.1021/jacs.1c05468

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An electrochemical protease assay using ferrocenylpeptide for screening of periodontal disease 査読有り国際誌

Sato S., Nagata S., Shimamoto J., Okinaga T., Ariyoshi W., Usui M., Nakashima K., Nishihara T., Takenaka S.

Bunseki Kagaku ( 公益社団法人日本分析化学会 ) 70 ( 3 ) 199 - 206 2021年01月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Porphyromonas gingivalis, known as an anaerobic gram-negative bacteria, is associated with the progress of a periodontitis. Since gingipain (gp), a specific protease secreted from its bacteria, is its main pathogenicity, gp has been utilized as a marker of the periodontitis. Here, an electrochemical protease assay using a ferrocenylpeptide probes was applied to the detection of gp activity; the probe was cleaved with its protease activity and current peak decreased by the released ferrocene part to balk solution. A series of ferrocenylpeptides as specific substrates of Arg-gp (Rgp) and Lys-gp (Kgp) was synthesized: FRC and FKC having cysteine residue; FRSS and FKSS having lipoic acid; FRpra and FKpra having propargylglycine; FRDE and FRDK having cysteine and D-type amino acid. The electron transfer rate constant of all of ferrocenylpeptide immobilized on a gold electrode were estimated by Lavirons analysis. The obtained electron transfer rate constant were 400-3500 s-1, where fastest one of 3500 s-1 was obtained from FRC immobilized electrode. Peak currents of these electrodes were decreased after the treatment of a sample containing Porphyromonas gingivalis. The detection limits of Porphyromonas gingivalis were 1.0×107, 5.0×106, 3.3×106, 5.0×106, and 5.0×106 cells, for FRPra- FKPra-, FRC-, FRDE-, and FRDK-immobilized electrodes, respectively. FRDE- and FRDK-immobilized electrodes were applied for patients suffering from periodontitis. It was successfully classified between the patients and healthy peoples using the FRDK-immobilized electrode. These results suggest that gp activity is connected with a clinical condition of periodontitis, and it is expected to be applied to a periodontal disease screening.

DOI： 10.2116/bunsekikagaku.70.199

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Electrochemical sensory detection of: Sus scrofa mtDNA for food adulteration using hybrid ferrocenylnaphthalene diimide intercalator as a hybridization indicator 査読有り国際誌

Kusnin N., Yusof N.A., Abdullah J., Sabri S., Mohammad F., Mustafa S., Ab Mutalib N.A., Sato S., Takenaka S., Parmin N.A., Al-Lohedan H.A.

RSC Advances 10 ( 46 ) 27336 - 27345 2020年06月

記述言語：英語掲載種別：研究論文（学術雑誌）

© The Royal Society of Chemistry. In this study, an electrochemical DNA biosensor was developed based on the fabrication of silicon nanowires/platinum nanoparticles (SiNWs/PtNPs) on a screen-printed carbon electrode (SPCE) for the detection of Sus scrofa mitochondrial DNA (mtDNA) in food utilizing a new hybrid indicator, ferrocenylnaphthalene diimide (FND). The morphology and elemental composition of the SiNWs/PtNPs-modified SPCE was analyzed by field emission scanning electron microscopy (FESEM) combined with energy dispersive X-ray spectroscopy (EDX). Cyclic voltammetry (CV) was used to study the electrical contact between the PtNPs and the screen-printed working electrode through SiNWs, while electrochemical impedance spectroscopy (EIS) was used to measure the charge transfer resistance of the modified electrode. The results clearly showed that the SiNWs/PtNPs were successfully coated onto the electrode and the effective surface area for the SiNWs/PtNPs-modified SPCE was increased 16.8 times as compared with that of the bare SPCE. Differential pulse voltammetry used for the detection of porcine DNA with FND as an intercalator confirmed its specific binding to the double-stranded DNA (dsDNA) sequences. The developed biosensor showed a selective response towards complementary target DNA and was able to distinguish non-complementary and mismatched DNA oligonucleotides. The SiNWs/PtNPs-modified SPCE that was fortified with DNA hybridization demonstrated good linearity in the range of 3 × 10-9 M to 3 × 10-5 M (R2 = 0.96) with a detection limit of 2.4 × 10-9 M. A cross-reactivity study against various types of meat and processed food showed good reliability for porcine samples.

DOI： 10.1039/d0ra03585h

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The interaction of cyclic naphthalene diimide with G-quadruplex under molecular crowding condition 招待有り査読有り国際誌

Zou T., Sato S., Yasukawa R., Takeuchi R., Ozaki S., Fujii S., Takenaka S.

Molecules 25 ( 3 ) 2020年02月

記述言語：英語掲載種別：研究論文（学術雑誌）

© 2020 by the authors. G-quadruplex specific targeting molecules, also termed as G4 ligands, are attracting increasing attention for their ability to recognize and stabilize G-quadruplex and high potentiality for biological regulation. However, G4 ligands recognizing G-quadruplex were generally investigated within a dilute condition, which might be interfered with under a cellular crowding environment. Here, we designed and synthesized several new cyclic naphthalene diimide (cNDI) derivatives, and investigated their interaction with G-quadruplex under molecular crowding condition (40% v/v polyethylene glycol (PEG)200) to mimic the cellular condition. The results indicated that, under molecular crowding conditions, cNDI derivatives were still able to recognize and stabilize G-quadruplex structures based on circular dichroism measurement. The binding affinities were slightly decreased but still comparatively high upon determination by isothermal titration calorimetry and UV-vis absorbance spectroscopy. More interestingly, cNDI derivatives were observed with preference to induce a telomere sequence to form a hybrid G-quadruplex under cation-deficient molecular crowding conditions.

DOI： 10.3390/molecules25030668

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Cyclic Naphthalene Diimide with a Ferrocene Moiety as a Redox-Active Tetraplex-DNA Ligand 査読有り国際誌

Kaneyoshi S., Zou T., Ozaki S., Takeuchi R., Udou A., Nakahara T., Fujimoto K., Fujii S., Sato S., Takenaka S.

Chemistry - A European Journal 26 ( 1 ) 139 - 142 2020年01月

記述言語：英語掲載種別：研究論文（学術雑誌）

© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Cyclic naphthalene diimides (cNDIs), with a ferrocene moiety (cFNDs) and different linker lengths between the ferrocene and cNDI moieties, were designed and synthesized as redox-active, tetraplex-DNA ligands. Intramolecular stacking was observed between ferrocene and the NDI planes, which could affect the binding properties for G-quadruplexes. Interestingly, the circular dichroism spectrum of one of these compounds clearly shows new Cotton effects around 320–380 and 240 nm, which can be considered a direct evidence of intramolecular stacking of ferrocene and the NDI. Regarding recognition of hybrid G-quadruplexes, the less rigid structures (longer linkers) show higher binding affinity (106 m−1 order of magnitude). All new compounds show higher selectivity for G4 during electrochemical detection than noncyclic FND derivatives, which further identifies the redox-active potentiality of the cFNDs. Two of the three compounds tested even show preferential inhibition of cell growth in cancer cells over normal cells in a low concentration range, highlighting the potential for bioapplications of these cFNDs.

DOI： 10.1002/chem.201903883

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Naphthalene diimide carrying four ferrocenyl substitutes as an electrochemical indicator of tetraplex DNA aiming at cancer diagnosis 査読有り国際誌

Sato S., Kajima A., Hamanaka H., Takenaka S.

Journal of Organometallic Chemistry 897 107 - 113 2019年10月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

© 2019 Elsevier B.V. Naphthalene diimide carrying four ferrocenyl substituents, tFND1 and tFND2, were newly synthesized to expect the improved binding affinity for tetraplex DNA and specific electrochemical signal generation. tFND2 shows the 1:2 binding to tetraplex A-core DNA, which has a part of human telomere sequence based on circular dichroism spectra, and the current increase of tFND2 on the tetraplex DNA-immobilized electrode was 13.5-times higher than that on the single stranded DNA-immobilized electrode. When applied to an electrochemical telomerase assay (ECTA) for HeLa cell, tFND2 shows the quantitative change around 0.1–50 cells/μL and the detection limit of 0.1 cells/μL for HeLa cell.

DOI： 10.1016/j.jorganchem.2019.06.028

その他リンク： https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068254217&origin=inward

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