研究者詳細 - 佐藤　しのぶ

論文 - 佐藤　しのぶ

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Synthesis of naphthalenediimide having two beta-cyclodextrins at both of its substituent termini. 査読有り国際誌

Ohtsuka K., Sato S., Watanabe S., Komizo K., Takenaka S.

Nucleic acids symposium series (2004) ( 52 ) 717 - 718 2008年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Naphthalenediimide (1) carrying two beta-cyclodextrins (beta CDs) was successfully synthesized by click chemistry between pentynylnaphthalenediimide and azido beta CD. Absorption spectra of 1 showed hypochromic and red shifts upon addition of sonicated calf thymus DNA as a double stranded DNA. Kinetic studies showed its slow association with and dissociation from calf thymus DNA. These results suggested that 1 can bind to double stranded DNA by the threading mode, where one of the beta CD is required to go through adjacent base pairs of double stranded DNA. If this is proven, beta CD is one of the largest groups ever reported which can go through a DNA duplex for threading.

DOI： 10.1093/nass/nrn362

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Synthesis of a perylene diimide derivative having two ferrocene moieties as an electrochemical indicator for human telomeric DNA tetraplex. 査読有り国際誌

Fujikawa R., Watanabe S., Ohtsuka K., Sato S., Takenaka S.

Nucleic acids symposium series (2004) ( 52 ) 241 - 242 2008年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

A water-soluble perylene diimide derivative carrying two ferrocene moieties, FPD, was synthesized in two steps as an electrochemical indicator for human telomeric DNA tetraplex. Spectrophotometric binding studies of FPD with AG(3)(T(2)AG(3))(3) revealed its specific binding to tetraplex DNA in 10 mM MES and 1 mM EDTA (pH 6.25) containing 0.10 M KCl. Cyclic voltammograms of AAT CCG TCG AGC AGA G(T(2)AG(3))(4) in an electrolyte containing 20 mM FPN showed single oxidation and reduction peaks based on the ferrocene moieties. The peak current for the oxidation process increased linearly with the scan rate, suggesting the effective concentration of FPN on this electrode.

DOI： 10.1093/nass/nrn122

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Synthesis of a naphthalene diimide derivative having four ferrocene moieties as an electrochemical DNA hybridization indicator. 査読有り国際誌

Sato S., Tsueda M., Watanabe S., Ohtsuka K., Takenaka S.

Nucleic acids symposium series (2004) ( 52 ) 239 - 240 2008年12月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

A new naphthalene diimide derivative carrying four ferrocene moieties, F(4)ND, was synthesized by the reaction of trimethylammoniummethylferrocene with the terminal amino moieties of two imide substituents of naphthalene diimide. Spectrophotometric binding studies of F(4)ND with calf thymus DNA showed its high affinity of 2 x 10(5) M(-1) in 10 mM MES buffer and 1 mM EDTA (pH 6.25) containing 0.10 M NaCl. The Osteryoung square wave voltammetry (SWV) of a DNA probe-immobilized electrode before and after hybridization with target DNA gave a larger peak current in an electrolyte containing F(4)ND than that containing FND carrying two ferrocene moieties. The current peak difference between mismatched and fully matched DNA was also greater with F(4)ND than that with FND.

DOI： 10.1093/nass/nrn121

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Electrochemical assay for deoxyribonuclease I activity 査読有り国際誌

Sato S., Fujita K., Kanazawa M., Mukumoto K., Ohtsuka K., Waki M., Takenaka S.

Analytical Biochemistry 381 ( 2 ) 233 - 239 2008年10月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

A thiolated oligonucleotide having three ferrocenes was immobilized on a gold electrode through the sulfur-gold linkage. This electrode showed a current response based on the redox reaction of the ferrocene moieties and this response was decreased after treatment with deoxyribonuclease I (DNase I), suggesting the disappearance of the ferrocene moieties on the electrode by the DNase I digestion. A linear correlation between i0 and i, which are current peaks before and after DNase I treatment, respectively, was observed and this slope was decreased with increase in the amount of DNase I. No current decrease was observed in the presence of EDTA or RNase A instead of DNase I. These results suggested that the current decrease responded specifically to the amount of DNase I and this electrode could be used for an electrochemical DNase I assay. Under the optimum conditions of DNase I digestion at 37 °C for 30 min, a quantitative analysis could be achieved in the range of 10-4-10-2 units/μl of DNase I. © 2008 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.ab.2008.07.014

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Synthesis of naphthalenediimide having two β-cyclodextrins at both of its substituent termini 査読有り

Keiichi Ohtsuka,Shinobu Sato,Sadayoshi Watanabe,Kohei Komizo,Shigeori Takenaka

Nucleic Acids Symposium Series 52 717 - 718 2008年09月

Synthesis of a perylene diimide derivative having two ferrocene moieties as an electrochemical indicator for human telomeric DNA tetraplex 査読有り

Ryota Fujikawa,Sadayoshi Watanabe,Keiichi Ohtsuka,Shinobu Sato,Shigeori Takenaka

Nucleic Acids Symposium Series 52 241 - 242 2008年09月

Synthesis of a naphthalene diimide derivative having four ferrocene moieties as an electrochemical DNA hybridization indicator

Shinobu Sato,Masato Tsueda,Sadayoshi Watanabe,Keiichi Ohtsuka,Shigeori Takenaka

Nucleic Acids Symposium Series 52 239 - 240 2008年09月

Linker effect of ferrocenylnaphthalene diimide ligands in the interaction with double stranded DNA 査読有り

Shinobu Sato,Shigeori Takenaka

Journal of Organometallic Chemistry 693 ( 7 ) 1177 - 1185 2008年04月

Electrochemical Assay for deoxyribonuclease I Activity 査読有り

Shinobu Sato,Katsuya Fujita,Masanori Kanazawa,Kosuke Mukumoto,Keiichi Ohtsuka,Michinori Waki,Shigeori Takenaka

Analytical Biochemistry 381 233 - 239 2008年04月

Electrochemical RNase detection using ferrocenylnaphthalene diimide. 査読有り国際誌

Kanazawa M., Sato S., Ohtsuka K., Takenaka S.

Nucleic acids symposium series (2004) ( 51 ) 323 - 324 2007年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

To develop a conventional RNase detecting system, an RNA electrode was constructed by the immobilization of poly(A)+RNA from mouse kidney on the glassy carbon electrode. Electrochemical measurement using this RNA electrode in the electrolyte containing ferrocenylnaphthalene diimide (1) was carried out and showed the electrochemical signal depending on the amount of the immobilized RNA. After this electrode was treated with water, the differential pulse voltammetric (DPV) measurement was subsequently conducted in the electrolyte containing 1. When RNase is contained in the water, the electrochemical signal decreased with an increase of the amount of RNase. This is derived from the decreasing amount of RNA on the electrode by RNase. In DPV measurement, the concentration of RNaseA was linear in the range of 10(-10) - 10(-8) M and the amount of RNase in the tap water could be estimated.

DOI： 10.1093/nass/nrm162

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Searching study for the suitable ferrocenylnaphthalene diimide derivative in the electrochemical telomerase assay. 査読有り国際誌

Sato S., Ohtsuka K., Takenaka S.

Nucleic acids symposium series (2004) ( 51 ) 85 - 86 2007年12月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Interaction of ferrocenylnaphthalene diimide 1 with the tetraplex oligonucleotide, AGGG(TTAGGG)3, which is a part of human telomere sequence, was studied in 0.1 M AcONa-AcOH (pH 5.5) containing 0.1 M NaCl coupled with 22-meric single and double stranded oligonucleotides using spectrophotometric titration experiment to search for the more suitable tetraplex DNA-binding ligand to achieve the electrochemical telomerase assay. CD spectra and polyacrylamide gel electrophoresis revealed that this tetraplex oligonucleotide kept to the single conformational structure of basket type G-quadruplex under these conditions. Scatchard analysis showed that 1 can bind to the tetraplex oligonucleotide with the binding constant of 10(5) M(-1) order, which was the highest value among the other oligonucleotides. Binding number of 1 for these oligonucleotides were ca. 2, 11, and 3 for tetraplex, double stranded, and single stranded oligonucleotides, respectively. These values were reasonable when considering with the binding mode of 1 as a threading intercalator.

DOI： 10.1093/nass/nrm043

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Ferrocenylnaphthalene diimide-based electrochemical ribonuclease assay 査読有り

Kanazawa M., Kanazawa M., Sato S., Ohtsuka K., Takenaka S.

Analytical Sciences 23 ( 12 ) 1415 - 1419 2007年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

Messenger RNA (mRNA) poly(A)+RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3′-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2 - 10 pg of RNase A. 2007 © The Japan Society for Analytical Chemistry.

DOI： 10.2116/analsci.23.1415

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The ferrocenyl-naphthalene diimide-based electrochemical ribonuclease assay 査読有り

Masanori Kanazawa,Shinobu Sato,Keiichi Ohtsuka,Shigeori Takenaka

Analytical Science 23 ( 12 ) 1415 - 1419 2007年12月

Electorochemical RNaseA detection using ferrocenylnaphthalene diimide 査読有り

Masanori Kanazawa,Shinobu Sato,Keiichi Ohtsuka,Shigeori Takenaka

Nucleic Acids Symposium Series 51 323 - 324 2007年11月

Searching study for the suitable ferrocenylnaphthalene diimide derivative in the electrochemical telomerase assay 査読有り

Shinobu Sato,Keiichi Ohtsuka,Shigeori Takenaka

Nucleic Acids Symposium Series 51 85 - 86 2007年11月

Direct modification of mRNA by ferrocenyl carbodiimide and its application to electrochemical detection of mRNA 査読有り

Mukumoto K., Nojima T., Sato S., Waki M., Takenaka S.

Analytical Sciences 23 ( 1 ) 115 - 119 2007年01月

記述言語：英語掲載種別：研究論文（学術雑誌）

Ferrocenyl carbodiimide (1) could be used for the direct labeling of synthetic RNA and expressed mRNA in vitro with the electrochemically active ferrocene moieties. These RNAs modified by 1 could be detected electrochemically coupled with a DNA probe-immobilized electrode. After hybridization of 1.1 Kb mRNA modified by 1 with the DNA probeimmobilized electrode, the peak charge observed by an Osteryoung square wave voltammetry (SWV) measurement correlated well with the concentration of mRNA, having a detection limit at the sub nanogram level. 2007 © The Japan Society for Analytical Chemistry.

DOI： 10.2116/analsci.23.115

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Direct modification of mRNA by ferrocenylcarbodiimide and its application for electrochemical detection of mRNA 査読有り

Kosuke MUKUMOTO,Takahiko NOJIMA,Shinobu SATO,Michinori WAKI,Shigeori TAKENAKA

Analytical Sciences 23 ( 1 ) 115 - 119 2007年01月

Electrochemical detection of DNase I activity. 査読有り国際誌

Fujita K., Kanazawa M., Mukumoto K., Nojima T., Sato S., Kondo H., Waki M., Takenaka S.

Nucleic acids symposium series (2004) ( 50 ) 307 - 308 2006年12月

記述言語：英語掲載種別：研究論文（学術雑誌）

DNase I in one microl of the water could quantitate electrochemically with the detection limit of 0.01 units (ca. 20 pg) by using the ferrocenyl oligonucleotide-immobilized electrode prepared by thiolated oligonucleotide and ferrocenyl carbodiimide as a simple labeling reagent of redox unit.

DOI： 10.1093/nass/nrl153

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Linker chain effect of ferrocenylnaphthalene diimide derivatives on a tetraplex DNA binding. 査読有り国際誌

Sato S., Kondo H., Takenaka S.

Nucleic acids symposium series (2004) ( 50 ) 107 - 108 2006年12月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Spectrophotometric binding studies of a series of the naphthalene diimide derivatives, 1-7, carrying different chains with a human telomere oligonucleotide, d(TTAGG)(4) was carried out in 0.1 M AcOK-AcOH buffer (pH 5.6) and 0.1 M KCl. Under this condition, this DNA could exist as the mixture of two-type tetraplex structures and these derivatives could bind to this DNA with strong affinity of 10(6) M(-1). The effect of the linker chain is not so large in those binding affinity, but the ligand 5 having piperazine skeleton in the linker chain had relative higher affinity for this tetraplex DNA than other derivatives. Large hypochromic effect of these derivatives upon binding to the tetraplex DNA suggested that the binding mode of these derivatives might contribute the stacking interaction between the naphthalene diimide and guanine tetraplex planes.

DOI： 10.1093/nass/nrl053

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Electrochemical detection of DNase I activity 査読有り

Katsuya Fujita,Masanori Kanazawa,Kosuke Mukumoto,Takahiko Nojima,Shinobu Sato,Hiroki Kondo,Michinori Waki,Shigeori Takenaka

Nucleic Acids Symposium Series 50 307 - 308 2006年11月

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