研究者詳細 - 佐藤　しのぶ

論文 - 佐藤　しのぶ

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Nuclease activity detection using ferrocenyloligonucleotide-immobilized electrode 査読有り

S. Sato, S. Ohtsuka, S. Takenaka

ICAS2011 2011年05月

cancer diagnosis using FND-based electrochemical telomerase assay 招待有り査読有り

Shinobu Sato

Asian International Symposium 2011年03月

Discrimination of phosphorylated double stranded DNA by naphthalene diimide having zinc(II) dipicolylamine complexes 査読有り国際誌

Sadayoshi Watanabe,Keiichi Ohtsuka,Shinobu Sato,Shigeori Takenaka

Bioorg. Med. Chem. 19 ( 3 ) 1361 - 1365 2011年02月

記述言語：英語掲載種別：研究論文（学術雑誌）

Discrimination of phosphomonoesters and phosphodiesters of DNA was attempted with naphthalene diimide carrying two zinc-dipicolylamine (Dpa) units (1). The binding constant of 1 for a self-complementary octanucleotide was 1.3 × 106 M-1, while the value for the phosphorylated counterpart was 4.8 × 106 M-1. This fourfold increase in the binding constant seems to stem from higher affinity of the terminal monophosphate over the phosphodiesters of DNA as the fourth ligand for the metal in 1. Likewise, the binding constant of 1 for DNase I-treated calf thymus DNA (average size 200 bp) was twice as large as that for untreated DNA (1 kb), possibly because the terminal phosphate groups are five times abundant in the former. These findings provide a clue to developing a system where phosphomonoesters generated upon DNA nicking are discriminated specifically from intact phosphodiesters. © 2010 Elsevier Ltd. All rights reserved.

DOI： 10.1016/j.bmc.2010.10.056

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Electrochemical detection of telomerase activity using ferrocenylnaphthalene diimide 査読有り

Shinobu Sato, Kumiko Mori, Yusuke Fukunaga, Ryota Fujikawa, Kazuhiro Tominaga, Tatsuji Nishihara, Shigeori Takenaka

PACIFICHEM 2010 2010年12月

FND-based electrochemical telomerase assay aiming at tongue cancer diagnosis

Shinobu Sato

第三回バイオセンシング技術に関する日韓シンポジウム(3rd JKBT) 2010年12月

DNA immobilization on the gold electrode using naphtalene diimide derivatives having dithiolane or cysteine moieties 査読有り

Kosuke Yamamura, Shinobu Sato, Shigeori Takenaka

PACIFICHEM2010 2010年12月

Electrochemical detection of double stranded DNA in homogenous solution using new naphthalene diimdie having ferroccenes located adjacent to triazole parts 査読有り

Sadayoshi Watanabe, Keiichi Ohtsuka, Shinobu Sato, Shigeori Takenaka

PACIFICHEM2010 2010年12月

Electrochemical detection of aberrant methylated genes by using ferrocenylnaphthalene diimide 査読有り

Shinobu Sato, Yusuke Kanezaki, Shigeori Takenaka

PACIFICHEM2010 2010年12月

Electrochemical detection of nuclease activity with ferrocenyloligonucleotide-immobilized electrodes 査読有り

Shinobu Sato, Katsuya Fujita, Shota Ohtsuka, Shigeori Takenaka

PACIFICHEM2010 2010年12月

Tris-intercalator based on the naphthalene diimide skeleton: Its synthesis by Click chemistry and DNA interaction 査読有り

Keiichi Ohtsuka, Yasuhiro Hadano, Shinobu Sato, Shigeori Takenaka

ISNAC2010 2010年11月

Electrochemical detection of aberrant methylated genes by using ferrocenylnaphthalene diimide 査読有り

Sadayoshi Watanabe, Shinobu Sato, Shigeori Takenaka

ISNAC2010 2010年11月

Tongue cancer diagnosis by FND-based electrochemical telomerase assay 査読有り

Yusuke Fukunaga, Shinobu Sato, Kumiko Mori, Hiroshi Endo, Kimio Morimoto, Kazuhiro Tominaga, Tatsuji Nishihara, Shigeori Takenaka

ISNAC2010 2010年11月

Electrochemical detection of aberrant methylated gene using naphthalene diimide derivative carrying four ferrocene moieties 査読有り国際誌

Shinobu Sato,Masato Tsueda,Shigeori Takenaka

J. Organomet. Chem. 695 ( 15-16 ) 1858 - 1862 2010年07月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Naphthalene diimide derivative, F4ND, carrying four ferrocene moieties was synthesized as a new hybridization indicator for electrochemical gene detection using DNA probe-immobilized electrode. F4ND could bind to double stranded DNA with threading intercalation mode despite the existence of such bulky substituents. As result, an improved binding affinity due to slower dissociation from double stranded DNA was observed. F4ND could be used for electrochemical discrimination between methylated and unmethylated PCR product of CDH4 gene coupled with methylation specific DNA probe-immobilized electrode under the optimum condition. Synthesized naphthalene diimide derivative, F4ND, carrying four ferrocene moieties could bind to double stranded DNA with threading intercalation mode and be used for electrochemical discrimination between methylated and unmethylated PCR product of the bisulfate reated CDH4 gene coupled with methylation specific DNA probe-immobilized electrode. © 2010 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jorganchem.2010.04.034

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Naphtalene diimide derivatives having dithiolane or cysteine moieties as double stranded DNA-immobilization reagent on a gold surface 査読有り

Shinobu Sato, Kousuke Yamamura, Shigeori Takenaka

Asian Chemical Biology Conference 2010(ACBC2010) 2010年06月

Reliable ferrocenyloligonucleotide-immobilized electrodes and their application to electrochemical DNase I assay 査読有り

Shinobu Sato,Katsuya Fujita,Masanori Kanazawa,Kosuke Mukumoto,Keiichi Ohtsuka,Shigeori Takenaka

Analytica Chimica Acta 645 30 - 35 2010年04月

Selective immobilization of double stranded DNA on a gold surface through threading intercalation of a naphthalene diimide having dithiolane moieties 査読有り国際誌

Shinobu Sato,Akinori Hirano,Shigeori Takenaka

Analytica Chimica Acta 665 ( 1 ) 91 - 97 2010年04月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Newly synthesized naphthalene diimide 1 having two dithiolane moieties at its substituted termini bound to double stranded DNA by threading intercalation and the resulting complex was immobilized on the gold surface through a dithiolane-gold linkage as revealed by quartz crystal microbalance (QCM) experiments. DNA with 20-meric double stranded and 24-meric single stranded regions was indirectly immobilized on the gold electrode using this characteristic of 1. Hybridization efficiency was 92%, a value higher than 50% for a thiolated oligonucleotide under identical conditions. When this electrode was subjected to hybridization with a 124-meric target DNA in the presence of ferrocenylnaphthalene diimide (FND) as an electrochemical hybridization indicator, a large current increase was observed deriving from FND bound in the double stranded region newly formed between the probe and target DNA. © 2010 Elsevier B.V.

DOI： 10.1016/j.aca.2010.03.008

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Immobilization of a naphthalene diimide-DNA complex on the gold through dithiolane moieties. 査読有り国際誌

Sato S., Ohtsuka K., Takenaka S.

Nucleic acids symposium series (2004) ( 53 ) 147 - 148 2009年12月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Naphthalene diimide 1 having two dithiolane moieties at its substituted termini was newly synthesized to immobilize double stranded DNA through dithiolane moieties of 1. Double stranded DNA could be immobilized on the gold surface of a quartz crystal microbalance (QCM) chip after binding 1. Once immobilized, the complex will serve as a hybridization marker based on its frequency decrease. QCM experiments showed that a DNA duplex with a 24-meric single stranded region also could be immobilized on the gold surface of a QCM chip and subsequent frequency decrease was observed after hybridization with a 24-meric complementary DNA, but not with non-complementary DNA, indicating specific hybridization. This result reveals that 1 provides a new immobilization method for intact DNA on gold and that the immobilized DNA can hybridize with target DNA.

DOI： 10.1093/nass/nrp074

Scopus

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Immobilization of a naphthalene diimide-DNA complex on the gold through dithiolane moieties 査読有り

Shinobu Sato,Keiichi Ohtsuka,Shigeori Takenaka

Nucleic Acids Symposium Series 53 147 - 148 2009年11月

Reliable ferrocenyloligonucleotide-immobilized electrodes and their application to electrochemical DNase I assay 査読有り

Sato S., Fujita K., Kanazawa M., Kanazawa M., Mukumoto K., Ohtsuka K., Takenaka S.

Analytica Chimica Acta 645 ( 1-2 ) 30 - 35 2009年07月

記述言語：英語掲載種別：研究論文（学術雑誌）

A ferrocenyloligonucleotide (FcODN) having contiguous cytosine bases was immobilized effectively and reproducibly on a gold electrode furnished with a self-assembled monolayer (SAM) having an N-hydroxysuccinimide-activated carboxylic acid. The resulting electrode was used as a sensor chip in DNase I assay. Thus, the current response of the modified electrode decreased upon addition of DNase I, demonstrating that the phosphodiester bonds of FcODN were cleaved. The DNase I activity assessed by Δi defined as (i0 - i)/i0, where i0 and i represent the current before and after DNase I treatment, respectively, was found to be reproducible with a standard deviation not greater than 9%. The DNase I can be quantitated in the range of 10-5 to 10-3 units μL-1 with a detection limit of 10-5 units μL-1 with this sensor chip. The current signal of the FcODN electrode was stable to storage in Biopak water up to 16 days with a 30% signal decrease over this period. DNase I activity in human sera was also determined successfully with this sensor chip. © 2009 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.aca.2009.04.047

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Electrochemical detection of deoxyribonuclease in microliquid 査読有り

Sakata Y., Hirokawa S., Sato S., Takenaka S., Yasuda T.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences 305 - 307 2009年01月

記述言語：英語掲載種別：研究論文（国際会議プロシーディングス）

This paper presents a novel microdevice which detects deoxyribonuclease (DNase) electrochemically. The present device can be applied to diagnoses of diseases such as acute myocardial infarction and monitoring of DNase-free water used in genetic research. The device consists of a PDMS reservoir for storing a sample liquid and several microelectrodes including working electrodes where ferrocenyloligonucleotides (DNA strands carrying ferrocene molecules) are immobilized. DNase detection is achieved by the current reduction between before and after ferrocenyloligonucleotide cleavage by DNase. Experimental results showed a definite correlation between DNase concentration and the current reduction rate in the range of 10-4 to 10-1 unit/μl of DNase I. © 2009 CBMS.

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